The potential of various Kribbella, Williamsia, and Tsukamurella species for azo dye decolorization

Mürüvvet Beyza Kömez; Zeynep Ulusoy; Nisa Gül Kaya; Ali Osman Adıgüzel

The potential of various Kribbella, Williamsia, and Tsukamurella species for azo dye decolorization

Abstract

The extensive use of synthetic dyes in the textile industry has resulted in the continuous discharge of toxic effluents into aquatic ecosystems. Among them, azo dyes represent the largest class of synthetic colorants and are known for their high stability and resistance to conventional physicochemical treatments. In this study, the dye-degrading potential of actinobacterial isolates maintained in the culture collection of the Ondokuz Mayıs University was evaluated using both solid and liquid media containing different azo dyes. During preliminary screening on dye-containing agar, all isolates exhibited good growth in the presence of dyes prepared at a concentration of 100 mg/L. Among these, when tested with MR, Kribbella hippodromi, Williamsia limnetica, Kribbella aluminosa, K. albertanoniae, Tsukamurella tyrosinosolvens, T. pulmonis, and T. strandjordii produced slight decolourisation zones. Distinct clearing zones on CSB-A agar were observed around T. spumae, T. tyrosinosolvens, T. inchonensis, and T. pulmonis. In the presence of FB, some strains exhibited strong decolourisation activity; however, FB partially inhibited the growth of certain isolates. Quantitative assays in liquid culture confirmed that W. limnetica achieved more than 90% removal of MR within 15 days, followed by K. aluminosa and T. tyrosinosolvens (87–92%). In contrast, the removal of CSB-A, SSBR, and CG-NP remained below 20%. Overall, MR and FB were the most susceptible dyes, and W. limnetica, T. tyrosinosolvens, and K. aluminosa emerged as the most efficient degraders. These findings highlight the broad-spectrum decolourisation capacity of actinobacteria and their promising potential for the bioremediation of dye-contaminated wastewater.

Keywords

Project Number

TUBITAK (2209), 1919B012431453

Ethical Statement

In the study, the authors declare that there is no violation of research and publication ethics and that the study does not require ethics committee approval.

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Details

Primary Language

English

Subjects

Industrial Microbiology

Journal Section

Research Article

Authors

Mürüvvet Beyza Kömez
0009-0000-1675-2395
Türkiye

Zeynep Ulusoy
0009-0001-4263-7611
Türkiye

Nisa Gül Kaya
0000-0003-2911-9876
Türkiye

Ali Osman Adıgüzel ^*
0000-0002-5602-5886
Türkiye

Early Pub Date

December 26, 2025

Publication Date

December 29, 2025

Submission Date

October 15, 2025

Acceptance Date

December 5, 2025

Published in Issue

Year 2025 Volume: 1 Number: 1

IZ

https://izlik.org/JA98SE87BM

Cite

RIS / Bibtex

APA

Kömez, M. B., Ulusoy, Z., Kaya, N. G., & Adıgüzel, A. O. (2025). The potential of various Kribbella, Williamsia, and Tsukamurella species for azo dye decolorization. Mediterranean Journal of Engineering and Scientific Research, 1(1), 21-30. https://izlik.org/JA98SE87BM