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Comparison of Some Hematological and Biochemical Analysis, ANAE Profile and Nasal Exfoliation Before and After the Race in jumping Horses in Bishkek Region in Kyrgyzstan

Year 2017, Volume: 7 Issue: 1, 12 - 20, 01.06.2017

Abstract

Çalışma Bişkek bölgesi konkur at yarışlarında yarışan spor atlarının yarış öncesi ve sonrası bazı hematolojik (WBC, RBC, HGB, HCT, MCV, MCH, MCHC, RDW-CV, RDW-SD, PLT, MPV, PDW, PCT) bazı biyokimyasal değerler (kan glukoz, ALT, ALP, AST, LDH, üre, GGT, CK, kreatinin ile ANAE pozitif lenfosit oranlarının belirlenmesi ve yarış öncesi ve sonrası nazal eksfolyasyonun değerlendirilmesi amacıyla yapılmıştır.Bu amaçla yarış hayvanlarından yarışma öncesi ve hemen sonrasında kan örnekleri EDTA’lı ve normal tüplere alınarak belirlenen yöntemlerle hematolojik ve biyokimyasal değerlendirmeler yapıldı. Yine usulüne uygun olarak ANAE pozitif lenfosit oranları belirlendi ve nazal eksfolyasyonu değerlendirmek amacıyla burun svapları alındı. Tüm veriler istatistiksel olarak karşılaştırıldı.Çalışma sonunda hematolojik verilerden HCT ve MPV değerlerinde, biyokimyasal analizlerde ise serum glukoz düzeyinde farklılık bulundu. ANAE pozitif lenfosit oranların da ANAE profilinin yarış peformansından etkilendiği görüldü. Biyokimyasal veriler değerlendirildiğinde ise serum glukoz seviyesinin değiştiği ve diğer biyokimyasal değerlerin korunduğu, nazal eksfolyasyonda da bir farklılık görülmediği belirlendi

References

  • Fazio, F., Messina, V., Casella, S., Giannetto, C., Marafioti, S., Piccione, G. (2012). Effect of a simulate show jumping competition on the blood gas profile of horses trained for show jumping. Turk J Vet Anim Sci, 36, 259–265.
  • Barrey, E., Valette, J.P. (1993). Exercise-related parameters of horses competing in show jumping events ranging from a regional to an international level. Ann zootech, 42, 89-98.
  • Dominik, B., Nicholas, S., Oriol, J., Brian, E., Lea, G., Alba, B., Alessandra, B. (2015). Comparative analysis of meteorological performance of coupled chemistry-meteorology models in the context of AQMEII phase 2. Atmospheric Environ, 115, 470–498.
  • Güzelbekteş, H., Ok, M., Şen, I., Coşkun, A. (2006). Atlarda uzun süreli fiziksel egzersizin hematolojik ve bazi biyokimyasal parametreler üzerine etkisi. Vet Bil Derg, 22, 27-30.
  • Keadle, T.L., Pourciau, S.S., Melrose, P.A., Kammerling, S.G., Horohov, D.W. (1999). Acute exercises stress modulates immune function in unfit horses. JVSE, 13, 226-231.
  • Robson, P.J., Alston, T.D., Myburgh, K.H. (2003). Prolonged suppression of the innate immune system in the horse following an 80 km endurance race. Equine Vet J, 35, 133-7.
  • Sur, E., Celik, I., Oznurlu, Y., Aydin, M.F., Sen, I., Ozparlak, H. (2003). Enzyme histochemistry and AgNOR numbers in the peripheral blood leukocytes of 6 month-old Kangal bred Anatolian shepherd dogs. Rev Med Vet, 154, 591 – 598.
  • Ergun, L., Ozen, A., Ergun, E., Asti, R.N. (2004). Alpha naphthyl acetate esterase activity in the peripheral blood leukocytes of turkeys. Indian Vet J, 81, 431-434.
  • Ozaydın, T., Çelik, İ., Sur, E., Öznurlu, Y., Uluışık, D. (2013). Cytochemistry of peripheral blood leukocytes in thorougbred foals. Biotech Histochem, 88, 295-301.
  • Hodgson, D.R., Rose, R.J. (1994). Training regimens: physiologic adaptations to training. Eds, Hodgson, D.R., Rose, R.J. In the athletic horse: Principles and practice of equine sports medicine, 379–385, WB Saunders: Philadelphia, USA.
  • Goodship, A.E., Birch, H. (2001). Exercise effects on the skeletal tissues. Eds, Back, W., Clayton, H.M. In: Equine Locomotion, 227–250, WB Saunders: London, England.
  • Sighieri, C., Masini, A.P., Baragli, P., Pasquini, A., Gavazza, A., Tedeschi, D., Martelli, F. (1999). Neutrophil-lymphocyte ratio in horses performing standardized incremental treadmill exercise. Ann Fac Med Vet Univ Pisa, 52, 77–83.
  • Nesse, L.L., Johansen, G.I., Blom, A.K. (2002). Effects of racing on lymphocyte proliferation in horses. Am J Vet Res, 63, 528-530.
  • Satué, K., Hernández, A., Muñoz, A. (2012). Physiological Factors in the Interpretation of Equine Hematological Profile. Eds, Lawrie, C. Hematology - Science and Practice, ISBN: 978-953-51-0174-1.
  • Allaam, M., Elseady, Y., Nayel, M., Elsify, A., Salama, A., Hassan, H., Kamar, A. (2014). Physiological and hemato-chemical evaluation of thoroughbred race horse after exercise. IJAVMS, 8, 81-93.
  • Piccione, G., Giannetto, C., Fazio, F., Di, Mauro, S., Caola, G. (2007). Haematological response to different workload in jumper horses. Bulg J Vet Med, 10, 21-28.
  • Mcgowan, T., Golland, C.M., Evans, L.C., Hodgson, D.L., Rose, R.J. (1999). Haematological and biochemical responses to training and overtraining. Equine Vet J, 31, 621-625.
  • Burlikowska, K., Bogusławska-Tryk, M., Szymeczko, R., Piotrowska, A. (2015). Haematological and biochemical blood parameters in horses used for sport and recreation. JCEA, 16, 370-382.
  • Revington, M. (1983). Haematology of the racing Thoroughbred in Australia 1: reference values and the effect of excitement. Equine Vet J, 15, 141-144.
  • Snow, D.H., Ricketts, S.W., Mason, D.K. (1983). Haematological response to racing and training exercise in Thoroughbred horses, with particular reference to the leucocyte response. Equine Vet J, 15, 149-154.
  • Uchakin, P.N., Gotovtseva, E.P., Stray-Gundersen, J. (2003). Immune and neuroendocrine alterations in marathon runners. J Appl Res, 3, 483–494.
  • Menge, C., Dean-Nystrom, E.A. (2008). Dexamethasone depletes γδT cells and alters the activation state and responsiveness of bovine peripheral blood lymphocyte subpopulations. J Dairy Sci, 91, 2284–2298.
  • Malinowski, K., Kearns, C.F., Guirnalda, P.D., Roegner, V., McKeever, K.H. (2004). Effect of chronic clenbuterol administration and exercise training on immune function in horses. J Anim Sci, 82, 3500–3507.
  • Catowsky, D. (1991). Practical Hematology, Eds, Livingstone, C., 125-155, Edinburgh, UK.
  • Tygstrup, N. (1990). Assessment of Liver Function: Principles and Practice. J Gastroenterol Hepatol, 5, 468–82.
  • Dufour, D.R., Loft, J.A., Nolte, F.S., Gretch, D.R., Koff, R.S., Seeff, L.B. (2000). Diagnosis and monitoring of hepatic ınjury. II. recommendations for use of laboratory tests in screening, diagnosis, and monitoring. Clinical Chemist, 46, 2050–68.
  • McIntyre, N. (2004). Clinical biochemistry of the liver. Principles of Medical Biology 15, 291–316.
  • Giannini, E.G., Testa, R., Savarino, V. (2005). Liver enzyme alteration: a guide for clinicians. CMAJ, 172, 367–79.
  • Lee, D.H., Blomhoff, R., Jacobs, D.R. (2004). Is serum gamma glutamyltransferase a marker of oxidative stress? Free Rad Res, 38, 535–39.
  • Arslan, M., Ozcan, M., Tosun, C., Cotelioglu, U., Matur, E. (2002). The effects of physical exercise on some plasma enzymes and Ca and P levels in race horses. İst Univ Vet Fak Derg, 28, 91-97.
  • Silva, J.F., Pannall, P.R. (1987). Tanı ve Tedavide Klinik Biyokimya. Çev: Tuncay Özgünen. Ikinci baskı.
  • Wallach, J. (2000). Interpretation of Diagnostic Tests. Seventh edition, PA: Lippincott Williams & Wilkins, Philadelphia, USA.
  • Weidner, N. (1982). Laboratory diagnosis of acute myocardial infarct: usefulness of determination of lactate dehydrogenase (LDH)-1 level and of ratio of LDH-1 to total LDH. Arch Pathol Lab Med,106, 375-7.
  • Graafroel-Fsema, E.D., Ginneken, M.E., Breda, E.V., Wijnberg, I.D., Keizer, H.A., Kolk, J. (2006). The effect of long term exercise on glucose metabolism and peripheral insulin sensitivity in Standardbred horses. Equine Vet J, 38, 221-225.

Kırgızıstan Bişkek Yöresi Konkur (Engel) Atlarında Yarış Öncesi ve Sonrası Bazı Hematolojik, Biyokimyasal Analizler ile ANAE Profili ve Nazal Eksfolyasyonun Karşılaştırılması

Year 2017, Volume: 7 Issue: 1, 12 - 20, 01.06.2017

Abstract

Çalışma  Bişkek  bölgesi  konkur  at  yarışlarında  yarışan  spor  atlarının,  yarış  öncesi  ve  sonrası  bazı hematolojik  (WBC,  RBC,  HGB,  HCT,  MCV,  MCH,  MCHC,  RDW‐CV,  RDW‐SD,  PLT,  MPV,  PDW,  PCT)  ve biyokimyasal değerleri (Kan glukoz, ALT, ALP, AST, LDH, Üre, GGT, CK, Kreatinin) ile periferik kan ANAE pozitif  lenfosit  oranının  belirlenmesi,  ayrıca  yarış  öncesi  ve  sonrası  nazal  eksfolyasyonun değerlendirilmesi  amacıyla  yapılmıştır.  Bu  amaçla  konkur  atlarından  yarışma  öncesi  ve  hemen sonrasında kan örnekleri alınarak hematolojik ve biyokimyasal ölçümler yapıldı. Bununla birlikte periferik kan  ANAE  pozitif  lenfosit  oranları  belirlendi  ve  istatistiksel  olarak  karşılaştırıldı.  Nazal  eksfolyasyonu değerlendirmek  amacıyla  burun  svapları  alındı.  Çalışma  sonunda  yarışma  öncesi  ile  sonrası karşılaştırıldığında;  HCT,  MPV,  serum  glukoz  ve  periferik  kan  ANAE  pozitif  lenfosit  oranda  farklılık saptandı. Yarış öncesi ve sonrasında alınan nazal eksfolyasyon örneklerinde farklılık görülmedi. Anahtar Kelimeler: ANAE, hematoloji, konkur atı, nazal eksfolyasyon, serum biyokimya      Comparison of Some Hematological and Biochemical Analysis, ANAE Profile and Nasal Exfoliation Before and After the Race in jumping Horses in Bishkek Region in Kyrgyzstan

References

  • Fazio, F., Messina, V., Casella, S., Giannetto, C., Marafioti, S., Piccione, G. (2012). Effect of a simulate show jumping competition on the blood gas profile of horses trained for show jumping. Turk J Vet Anim Sci, 36, 259–265.
  • Barrey, E., Valette, J.P. (1993). Exercise-related parameters of horses competing in show jumping events ranging from a regional to an international level. Ann zootech, 42, 89-98.
  • Dominik, B., Nicholas, S., Oriol, J., Brian, E., Lea, G., Alba, B., Alessandra, B. (2015). Comparative analysis of meteorological performance of coupled chemistry-meteorology models in the context of AQMEII phase 2. Atmospheric Environ, 115, 470–498.
  • Güzelbekteş, H., Ok, M., Şen, I., Coşkun, A. (2006). Atlarda uzun süreli fiziksel egzersizin hematolojik ve bazi biyokimyasal parametreler üzerine etkisi. Vet Bil Derg, 22, 27-30.
  • Keadle, T.L., Pourciau, S.S., Melrose, P.A., Kammerling, S.G., Horohov, D.W. (1999). Acute exercises stress modulates immune function in unfit horses. JVSE, 13, 226-231.
  • Robson, P.J., Alston, T.D., Myburgh, K.H. (2003). Prolonged suppression of the innate immune system in the horse following an 80 km endurance race. Equine Vet J, 35, 133-7.
  • Sur, E., Celik, I., Oznurlu, Y., Aydin, M.F., Sen, I., Ozparlak, H. (2003). Enzyme histochemistry and AgNOR numbers in the peripheral blood leukocytes of 6 month-old Kangal bred Anatolian shepherd dogs. Rev Med Vet, 154, 591 – 598.
  • Ergun, L., Ozen, A., Ergun, E., Asti, R.N. (2004). Alpha naphthyl acetate esterase activity in the peripheral blood leukocytes of turkeys. Indian Vet J, 81, 431-434.
  • Ozaydın, T., Çelik, İ., Sur, E., Öznurlu, Y., Uluışık, D. (2013). Cytochemistry of peripheral blood leukocytes in thorougbred foals. Biotech Histochem, 88, 295-301.
  • Hodgson, D.R., Rose, R.J. (1994). Training regimens: physiologic adaptations to training. Eds, Hodgson, D.R., Rose, R.J. In the athletic horse: Principles and practice of equine sports medicine, 379–385, WB Saunders: Philadelphia, USA.
  • Goodship, A.E., Birch, H. (2001). Exercise effects on the skeletal tissues. Eds, Back, W., Clayton, H.M. In: Equine Locomotion, 227–250, WB Saunders: London, England.
  • Sighieri, C., Masini, A.P., Baragli, P., Pasquini, A., Gavazza, A., Tedeschi, D., Martelli, F. (1999). Neutrophil-lymphocyte ratio in horses performing standardized incremental treadmill exercise. Ann Fac Med Vet Univ Pisa, 52, 77–83.
  • Nesse, L.L., Johansen, G.I., Blom, A.K. (2002). Effects of racing on lymphocyte proliferation in horses. Am J Vet Res, 63, 528-530.
  • Satué, K., Hernández, A., Muñoz, A. (2012). Physiological Factors in the Interpretation of Equine Hematological Profile. Eds, Lawrie, C. Hematology - Science and Practice, ISBN: 978-953-51-0174-1.
  • Allaam, M., Elseady, Y., Nayel, M., Elsify, A., Salama, A., Hassan, H., Kamar, A. (2014). Physiological and hemato-chemical evaluation of thoroughbred race horse after exercise. IJAVMS, 8, 81-93.
  • Piccione, G., Giannetto, C., Fazio, F., Di, Mauro, S., Caola, G. (2007). Haematological response to different workload in jumper horses. Bulg J Vet Med, 10, 21-28.
  • Mcgowan, T., Golland, C.M., Evans, L.C., Hodgson, D.L., Rose, R.J. (1999). Haematological and biochemical responses to training and overtraining. Equine Vet J, 31, 621-625.
  • Burlikowska, K., Bogusławska-Tryk, M., Szymeczko, R., Piotrowska, A. (2015). Haematological and biochemical blood parameters in horses used for sport and recreation. JCEA, 16, 370-382.
  • Revington, M. (1983). Haematology of the racing Thoroughbred in Australia 1: reference values and the effect of excitement. Equine Vet J, 15, 141-144.
  • Snow, D.H., Ricketts, S.W., Mason, D.K. (1983). Haematological response to racing and training exercise in Thoroughbred horses, with particular reference to the leucocyte response. Equine Vet J, 15, 149-154.
  • Uchakin, P.N., Gotovtseva, E.P., Stray-Gundersen, J. (2003). Immune and neuroendocrine alterations in marathon runners. J Appl Res, 3, 483–494.
  • Menge, C., Dean-Nystrom, E.A. (2008). Dexamethasone depletes γδT cells and alters the activation state and responsiveness of bovine peripheral blood lymphocyte subpopulations. J Dairy Sci, 91, 2284–2298.
  • Malinowski, K., Kearns, C.F., Guirnalda, P.D., Roegner, V., McKeever, K.H. (2004). Effect of chronic clenbuterol administration and exercise training on immune function in horses. J Anim Sci, 82, 3500–3507.
  • Catowsky, D. (1991). Practical Hematology, Eds, Livingstone, C., 125-155, Edinburgh, UK.
  • Tygstrup, N. (1990). Assessment of Liver Function: Principles and Practice. J Gastroenterol Hepatol, 5, 468–82.
  • Dufour, D.R., Loft, J.A., Nolte, F.S., Gretch, D.R., Koff, R.S., Seeff, L.B. (2000). Diagnosis and monitoring of hepatic ınjury. II. recommendations for use of laboratory tests in screening, diagnosis, and monitoring. Clinical Chemist, 46, 2050–68.
  • McIntyre, N. (2004). Clinical biochemistry of the liver. Principles of Medical Biology 15, 291–316.
  • Giannini, E.G., Testa, R., Savarino, V. (2005). Liver enzyme alteration: a guide for clinicians. CMAJ, 172, 367–79.
  • Lee, D.H., Blomhoff, R., Jacobs, D.R. (2004). Is serum gamma glutamyltransferase a marker of oxidative stress? Free Rad Res, 38, 535–39.
  • Arslan, M., Ozcan, M., Tosun, C., Cotelioglu, U., Matur, E. (2002). The effects of physical exercise on some plasma enzymes and Ca and P levels in race horses. İst Univ Vet Fak Derg, 28, 91-97.
  • Silva, J.F., Pannall, P.R. (1987). Tanı ve Tedavide Klinik Biyokimya. Çev: Tuncay Özgünen. Ikinci baskı.
  • Wallach, J. (2000). Interpretation of Diagnostic Tests. Seventh edition, PA: Lippincott Williams & Wilkins, Philadelphia, USA.
  • Weidner, N. (1982). Laboratory diagnosis of acute myocardial infarct: usefulness of determination of lactate dehydrogenase (LDH)-1 level and of ratio of LDH-1 to total LDH. Arch Pathol Lab Med,106, 375-7.
  • Graafroel-Fsema, E.D., Ginneken, M.E., Breda, E.V., Wijnberg, I.D., Keizer, H.A., Kolk, J. (2006). The effect of long term exercise on glucose metabolism and peripheral insulin sensitivity in Standardbred horses. Equine Vet J, 38, 221-225.
There are 34 citations in total.

Details

Other ID JA98UT78KC
Journal Section Research Article
Authors

Ertan Oruç This is me

İhsan Kısadere This is me

Nariste Kadıralıeva This is me

Emrah Sur This is me

Publication Date June 1, 2017
Submission Date June 1, 2017
Published in Issue Year 2017 Volume: 7 Issue: 1

Cite

APA Oruç, E., Kısadere, İ., Kadıralıeva, N., Sur, E. (2017). Comparison of Some Hematological and Biochemical Analysis, ANAE Profile and Nasal Exfoliation Before and After the Race in jumping Horses in Bishkek Region in Kyrgyzstan. Manas Journal of Agriculture Veterinary and Life Sciences, 7(1), 12-20.