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Year 2018, Volume: 8 Issue: 2, 17 - 24, 01.12.2018

Abstract

References

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  • Blanca, J., Canizares, J., Roig, C., Ziarsolo, P., Nuez, F. ve Pico, B., 2011, Transcriptome characterization and high throughput SSRs and SNPs discovery in Cucurbita pepo (Cucurbitaceae), Blanca et al. BMC Genomics, 12:104.
  • Can, H., Türkmen, Ö., Işık, R., Paksoy, M., Seymen, M., Fİdan, S. ve Hakkı, E. E., 2014, Çerezlik Kabak Hatlarının Saflıklarının Dominant Markör Sistemleriyle Belirlenmesi, Uluslararası katılımlı 5. Tohumculuk Kongresi Diyarbakır.
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  • (Brassica rapa L. ssp. pekinensis), Hindawi Publishing Corporation International Journal of Genomics, 2015.
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  • Gong, L., Paris, H. S., Stift, G., Lelley, T., Nee, M. H., Pachner, M. ve Vollmann, J., 2012, Genetic relationships and evolution in Cucurbita pepo (pumpkin, squash, gourd) as revealed by simple sequence repeat polymorphisms, Theoretical and Applied Genetics, 124, 875-891.
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  • Rahim, B., PeyvastGholamali, Ali, A. M., Babak, R., Akbar, E. A. ve Ali, B., 2013, Biochemical systematic, population structure and genetic variability studies among Iranian Cucurbita (Cucurbita pepo L.) accessions, using genomic SSRs and implications for their breeding potential, Biochemical Systematics and Ecology, 50, 187-198.
  • Robinson RW, Munger HM, Whitaker TW ve GW., B., 1976, Genes of the cucurbitaceae, Hortscience, 11, 554-568.
  • Seymen, M., Türkmen, Ö. ve Paksoy, M., 2012, Selection of edible pumpkin seeds (Cucurbita pepo L.) genotypes, Journal of Selçuk University Natural and Applied Science, 2(4), 29-39.
  • Seymen, M., Yavuz, D., Yavuz, N. ve Türkmen, Ö., 2016, Effect on Yield and Yield Components of Different Irrigation Levels in Edible Seed Pumpkin Growing, world academy of science engineering and technology, 10, 214-219.
  • Solmaz, İ., 2010 Karakterizasyonu Ve Fusarıum Solgunluğu (Fusarium Oxysporum F.Sp. Niveum)’na Dayanımlarının Klasik Ve Moleküler Yöntemlerle Araştırılması, Çukurova Üniversitesi, Adana.

Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu

Year 2018, Volume: 8 Issue: 2, 17 - 24, 01.12.2018

Abstract

This study was carried out to determine the molecular characterization of 83 well-established lines in the prospective S4 stage in the edible pumpkins (Cucurbita pepo L.). The molecular diversity among the pumpkins genotypes was determined by SSR markers. Thirty one EST- SSR markers yielded a total of 122 amplified DNA fragments, of which 107 (87.70%) were polymorphic, were used to measure the degree of genetic diversity. The DARwin software was used to generate a Dice coefficient dissimilarity matrix that was then used to construct an unweighted neighbor-joining dendrogram of the accessions. Neighbor-joining dendrogram demonstrated that the germplasm collection is divided into three main groups. The highest PIC value was calculated for the marker CUTC006209 as 0.42. According to the Mantel test results, correlation of the dissimilarity matrix and the dendrogram was 0.99 which is a very high score that indicates a very strong degree of correlation. Genetic relationships calculated based on molecular genetic data demonstrated the degree of diversity within the population along with the pattern of clustering among accessions which will provide valuable information to breeders for future breeding programs.

References

  • TÜİK, 2014. 26.03.2017. https://biruni.tuik.gov.tr/bitkiselapp/bitkisel.zul.
  • Blanca, J., Canizares, J., Roig, C., Ziarsolo, P., Nuez, F. ve Pico, B., 2011, Transcriptome characterization and high throughput SSRs and SNPs discovery in Cucurbita pepo (Cucurbitaceae), Blanca et al. BMC Genomics, 12:104.
  • Can, H., Türkmen, Ö., Işık, R., Paksoy, M., Seymen, M., Fİdan, S. ve Hakkı, E. E., 2014, Çerezlik Kabak Hatlarının Saflıklarının Dominant Markör Sistemleriyle Belirlenmesi, Uluslararası katılımlı 5. Tohumculuk Kongresi Diyarbakır.
  • Devran, Z., 2003, Moleküler İşaretleyicilerin (Markörlerin) Dayanıklılık Islahında Kullanılması, 20, 1-6.
  • Ding, Q., Li, J., Wang, F., Zhang, Y., Li, H., Zhang, J. ve Gao Jianwei., 2015, Characterization and Development of EST-SSRs by
  • (Brassica rapa L. ssp. pekinensis), Hindawi Publishing Corporation International Journal of Genomics, 2015.
  • Doyle, J. J. ve Doyle, J. L., 1987, A rapid DNA isolation procedure for small quantities of fresh leaf tissue, Phytochemical Bulletin, 19, 11-15.
  • Gong, L., Paris, H. S., Stift, G., Lelley, T., Nee, M. H., Pachner, M. ve Vollmann, J., 2012, Genetic relationships and evolution in Cucurbita pepo (pumpkin, squash, gourd) as revealed by simple sequence repeat polymorphisms, Theoretical and Applied Genetics, 124, 875-891.
  • OH, F., 1972, The Significance, Utilization and Conservation of Crop Genetic Resources, FAO, Rome.
  • Rahim, B., PeyvastGholamali, Ali, A. M., Babak, R., Akbar, E. A. ve Ali, B., 2013, Biochemical systematic, population structure and genetic variability studies among Iranian Cucurbita (Cucurbita pepo L.) accessions, using genomic SSRs and implications for their breeding potential, Biochemical Systematics and Ecology, 50, 187-198.
  • Robinson RW, Munger HM, Whitaker TW ve GW., B., 1976, Genes of the cucurbitaceae, Hortscience, 11, 554-568.
  • Seymen, M., Türkmen, Ö. ve Paksoy, M., 2012, Selection of edible pumpkin seeds (Cucurbita pepo L.) genotypes, Journal of Selçuk University Natural and Applied Science, 2(4), 29-39.
  • Seymen, M., Yavuz, D., Yavuz, N. ve Türkmen, Ö., 2016, Effect on Yield and Yield Components of Different Irrigation Levels in Edible Seed Pumpkin Growing, world academy of science engineering and technology, 10, 214-219.
  • Solmaz, İ., 2010 Karakterizasyonu Ve Fusarıum Solgunluğu (Fusarium Oxysporum F.Sp. Niveum)’na Dayanımlarının Klasik Ve Moleküler Yöntemlerle Araştırılması, Çukurova Üniversitesi, Adana.
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Details

Journal Section Articles
Authors

Necibe Kayak This is me

Önder Türkmen This is me

Ali Tevfik Uncu This is me

Yeşim Dal This is me

Publication Date December 1, 2018
Published in Issue Year 2018 Volume: 8 Issue: 2

Cite

APA Kayak, N., Türkmen, Ö., Uncu, A. T., Dal, Y. (2018). Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu. Manas Journal of Agriculture Veterinary and Life Sciences, 8(2), 17-24.