Investigation of the CAST Gene Polymorphism in Karayaka Sheep

Year 2021, Volume: 11 Issue: 1, 89 - 93, 01.06.2021

Koray Kırıkçı , Mehmet Cam , Levent Mercan

Abstract

 The study aimed to investigate the CAST gene's genetic polymorphism, known as a candidate gene for meat quality and quantities, using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method in Karayaka sheep. The blood samples were collected from 105 animals belong to four subpopulations (Samsun, Ordu, Giresun, and Tokat) in Turkey’s Black Sea region provinces. The genomic DNA was isolated by using an extraction kit. A fragment of 622 bp on CAST gene was amplified by PCR and then genotypes of the CAST gene for all individuals were determined with the restriction endonuclease MspI. At the end of the work, MM and MN’s genotypic frequencies were found to be 84 and 16% for the Karayaka breed. While the MN genotype was not observed only in the Ordu subpopulation, the NN genotype was not observed in any subpopulations studied. As a result, the obtained findings provided information about the CAST gene polymorphism for Karayaka breed at the population level. This result could be considered for genomic selection works to improve the meat quality traits of Karayaka in the future.

Keywords

Karayaka sheep, calpastatin gene, genetic variability, PCR-RFLP, MspI

Project Number

yok

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