Effect of Microbial Fertilizer on The Development of Pepper (Capsicum annuum L.) Seedlings Exposed to Salt Stress

Year 2023, Volume: 13 Issue: 1, 17 - 23, 01.07.2023

Halil İbrahim Öztürk , Atilla Dursun

https://doi.org/10.53518/mjavl.1237413

Cited By: 1

Abstract

Salinity is an important abiotic stress factor that negatively affects plant growth and yield. The study, the effect of microbial fertilizer containing Bacillus subtilis bacteria, commercially named Subtima, on salt stress in pepper was observed. Two different saline solutions (100 mM and 200 mM) were prepared to create salt stress in the plant growing medium. The fertilizer solution was applied to the plants in 4 different concentrations (300, 400, 500 and 600 ppm) from the leaves in the form of a spray. Morphological parameters such as plant height, stem length, leaf length, leaf width, and petiole length were measured. It was found that salt stress resulted in a decrease in plant morphological characteristics compared to the control group (0 mM salt + 0 ppm fertilizer). It was found that the most effective fertilizer dose to increase plant height (25.23 cm) at 100 mM salt stress was 400 ppm. At 200 mM salt stress, it was found that the most effective fertilizer dose for increasing plant height (22.67 cm) was an application of 300 ppm application. In general, fertilizer applications were found to be effective in reducing the negative effects of salt on the morphological traits studied.

Keywords

Abiotic stress, Bacillus subtilis, pepper, plant growing

References

• Abd El-Azeem, SA., Elwan, MW., Sung, JK., & Ok, YS. (2012). Alleviation of salt stress in eggplant (Solanum melongena L.) by plant-growth-promoting rhizobacteria. Commun. Soil Sci. Plant Anal. 43(9): 1303-1315.
• Altunlu, H. (2020). Tuz stresi altındaki biberde (Capsicum annuum L.) mikoriza ve rizobakteri uygulamasının bitki gelişimi ve bazı fizyolojik parametreler üzerine etkisi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 57(4): 501- 510.
• Amjad, M., Akhtar, J., Anwar-ul-Haq, M., Riaz, MA., Saqib, ZA., Murtaza, B., & Naeem, MA. (2016). Effectiveness of potassium in mitigating the salt-induced oxidative stress in contrasting tomato genotypes. J. Plant Nutr. 39(13): 1926-1935.
• Aroca, R., Porcel, R., & Ruiz-Lozano, JM. (2012). Regulation of root water uptake under abiotic stress conditions. Journal of experimental botany, 63(1): 43-57.
• Ayaz, M., Ali, Q., Jiang, Q., Wang, R., Wang, Z., Mu, G., Khan, AR., Manghwar, H., Wu, H., Gao, X., & Gu, Q. (2022). Salt tolerant bacillus strains improve plant growth traits and regulation of phytohormones in wheat under salinity stress. Plants, 11(20), 2769.
• Aydin, A., Çetin, AN., Başak, H., & Başpinar, A. 2022. Tuz stresi altındaki domates bitkilerine yapılan mikrobiyal (Glomus iranicum var. tenuihypharum ve Trichoderma Harzianum T78) uygulamaların morfolojik ve fizyolojik özellikler üzerine etkileri. Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi, 2(1): 32-42.
• Colla, G., Rouphael, Y., Leonardi, C., & Bie, Z. 2010. Role of grafting in vegetable crops grown under saline conditions. Scientia Horticulturae, 127(2): 147-155.
• Çirka, M., Tunçtürk, R., Kulaz, H., Tunçtürk, M., Eryiğit, T., & Baran, İ. (2022). Kuraklık stresi altında yetiştirilen bakla (Vicia faba L.) bitkisinde rizobakteri ve alg uygulamalarının bitki gelişimi üzerindeki etkilerinin incelenmesi. JIST. 12(2): 1124-1133.
• Del Amor, FM., Martinez, V., & Cerda, A. 2001. Salt tolerance of tomato plants as affected by stage of plant development. HortSci. 36(7): 1260-1263.
• El-Katatny, MH., Idres, MM. (2014). Effects of single and combined inoculations with Azospirillum brasilense and Trichoderma harzianum on seedling growth or yield parameters of wheat (Triticum vulgaris L., Giza 168) and corn (Zea mays L., hybrid 310). J. Plant Nutr. 37(12): 1913-1936.
• Grover, M., Ali, SZ., Sandhya, V., Rasul, A., & Venkateswarlu, B. (2011). Role of microorganisms in adaptation of agriculture crops to abiotic stresses. World J. Microbiol. Biotechnol. 27(5): 1231-1240.
• Hahm, MS., Son, JS., Hwang, YJ., Kwon, DK., Ghim, SY. (2017). Alleviation of salt stress in pepper (Capsicum annum L.) plants by plant growth-promoting rhizobacteria. J. Microbiol. Biotechnol. 27(10): 1790-1797.
• Hashem, A., Tabassum, B., & Abd_Allah, EF. (2019). Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi J. Biol. Sci. 26(6): 1291-1297.
• Huang, Z., He, CX., He, ZQ., Zou, ZR., & Zhang, ZB. (2010). The effects of arbuscular mycorrhizal fungi on reactive oxyradical scavenging system of tomato under salt tolerance. Agricultural Sciences in China, 9(8): 1150-1159.
• Li, C., Wang, P., Wei, Z., Liang, D., Liu, C., Yin, L., Jia, D., Fu, M., & Ma, F. (2012). The mitigation effects of exogenous melatonin on salinity‐induced stress in Malus hupehensis. J. Pineal Res. 53(3): 298-306.
• Loudari, A., Mayane, A., Zeroual, Y., Colinet, G., & Oukarroum, A. (2022). Photosynthetic performance and nutrient uptake under salt stress: Differential responses of wheat plants to contrasting phosphorus forms and rates. Front. Plant Sci. 13.
• Nizam, I. (2011). Effects of salinity stress on water uptake, germination and early seedling growth of perennial ryegrass. Afr. J. Biotechnol. 10(51): 10418-10424.
• Tuna, AL., & Eroğlu, B. (2017). Tuz stresi altindaki biber (Capsicum annuum L.) bitkisinde bazi organik ve inorganik bileşiklerin antioksidatif sisteme etkileri. Anadolu Tarım Bilimleri Dergisi, 32(1): 121-131.
• Wu, L., Huo, W., Yao, D., & Li, M. (2019). Effects of solid matrix priming (SMP) and salt stress on broccoli and cauliflower seed germination and early seedling growth. Sci. Hortic. 255, 161-168.
• Vivas, A., Marulanda, A., Ruiz-Lozano, JM., Barea, JM., & Azcón, R. (2003). Influence of a Bacillus sp. on physiological activities of two arbuscular mycorrhizal fungi and on plant responses to PEG-induced drought stress. Mycorrhiza, 13: 249-256.
• Yakupoğlu, G. (2020). Biberde tuz stresine karşı melatonin uygulamasının bazı fide özellikleri üzerine etkisi. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi, 36(1): 76-81.
• Yaman, M., Pınar, H., Seday, U., Altınöz, D., Uzun, A., & Çabuk, NS. (2020). In vitro screening for salt tolerance of some citrus rootstocks. Turkish Journal of Agriculture-Food Science and Technology, 8(5): 1117-1121.
• Yildiz, E., Yaman, M., Ercisli, S., Sumbul, A., Sonmez, O., Gunes, A., & Kviklys, D. (2022). Effects of rhizobacteria application on leaf and fruit nutrient content of different apple scion–rootstock combinations. Horticulturae, 8(6): 550.
• Zhang, H., Kim, MS., Sun, Y., Dowd, SE., Shi, H., & Paré, PW. (2008). Soil bacteria confer plant salt tolerance by tissue-specific regulation of the sodium transporter HKT1. Mol. Plant Microbe Interact. 21(6): 737-744.
• Zhang, T., Shi, Z., Zhang, X., Zheng, S., Wang, J., & Mo, J. (2020). Alleviating effects of exogenous melatonin on salt stress in cucumber. Sci. Hortic. 262, 109070.