Investigations on the Light and Scanning Electron Microscopic Structure of Tongue Papillae of Morkaraman Sheep: Taste and Mechanical

Year 2024, Volume: 14 Issue: 2, 167 - 175, 25.12.2024

Muhammed Demircioğlu , Muhammet Bahaeddin Dörtbudak , Funda Aksünger Karaavcı , Barış Can Güzel , İsmail Demircioğlu

https://doi.org/10.53518/mjavl.1501442

Abstract

In this study, the structure of the papillae in the tongue of Morkaraman sheep was investigated by light (LM) and scanning electron microscopy (SEM). The aim of this study was to investigate the morphology and topographic distribution of the lingual papillae of the tongues of Morkaraman sheep breed. Adult male Morkaraman sheep tongues were used in the study. Tongue samples for light and scanning electron microscopy were taken from the apex, corpus, radix and torus lingua regions of the upper surface of the tongue and the lower surface of the tongue tip. Two types of taste papillae, papillae fungiformis and vallata, were observed on the tongue. Papillae fungiformis were found under the tip of the tongue, on the upper surface and sides of the tip and body of the tongue and on torus lingua. Papillae vallata were observed on both posterior sides of the torus lingua of the tongue. SEM examination showed that the surface of the papillae was smooth and there were no taste pores opening to the surface. On LM examination, filiform papillae at the lingual apex were common in materials. These papillae had secondary keratin extensions on the main body. The light and scanning electron microscopic structures of the taste papillae on the tongue of Morkaraman sheep were examined and their similarities and differences with other ruminant species were determined.

Keywords

Morkaraman sheep, Papilla lingua, Scanning electron microscope, Tongue

Ethical Statement

The relevant article of law in our country states “Procedures with dead animals or tissues, slaughterhouse materials, waste fetuses are not subject to the permission of the Local Ethics Committee for Animal Experiments” in accordance with Article 8, paragraph eight (k) of the Regulation No. 28914 on the Working Procedures and Principles of Animal Experimentation Ethics Committees, the study materials are not subject to permission because they are taken from the slaughterhouse.

Supporting Institution

There is no institution funding the research

References

• Agungpriyono, S., Yamada, J., Kitamura, N., Nisa, C., Sigit, K., & Yamamoto, Y. (1995). Morphology of the dorsal lingual papillae in the lesser mouse deer, Tragulus javanicus. Journal of Anatomy, 187, 635.
• Akçapınar, H. (2000). Koyun yetiştiriciliği. İsmat Matbaacılık, Ankara.
• Baygeldi, S. B., Güzel, B.C., İlgün, R., & Özkan, E. (2023). Macroanatomy of cecum in german mast geese (Anser Anser) and investigation with scanning electron microscope (Sem). Brazilian Journal of Poultry Science, 25.
• Can, M., & Atalgın, Ş. H. (2015). Scanning electron microscopic study of the lingual papillae in the Anatolian water Buffalo. International Journal of Morphology, 33(3), 855–859.
• Can, M., Atalgın, Ş. H., Ateş, S., & Takçı, L. (2016). Scanning electron microscopic study on the structure of the lingual papillae of the Karacabey merino sheep. Eurasian Journal of Veterinary Sciences, 32(3), 130–135.
• Chamorro, C. A., De Paz, P., Sandoval, J., & Fernandez, J. G. (1986). Comparative scanning electron- microscopic study of the lingual papillae in two species of domestic mammals (Equus caballus and Bos taurus) 1. Gustatory papillae. Cells Tissues Organs, 125(2), 83-87.
• Emura, S., Tamada, A., Hayakawa, D., Chen, H., Yano, R., & Shoumura, S. (1999). Morphology of the dorsal lingual papillae in the blackbuck, Antilope cervicapra. Okajimas Folia Anatomica Japonica, 76(5), 247-253.
• Emura, S., Okumura, T., & Chen, H. (2011). Morphology of the lingual papillae in the roan antelope. Okajimas Folia Anatomica Japonica, 88(3): 127–131.
• El Sharaby, A. A., Alsafy, M. A., El‐Gendy, S. A., & Wakisaka, S. (2012). Morphological Characteristics of the Vallate Papillae of the One‐Humped C amel (C amelus dromedarius). Anatomia, Histologia, Embryologia, 41(6), 402-409.
• El Sharaby, A. A., El-Gendy, S. A., Alsafy, M. A., Nomir, A. G., & Wakisaka, S. (2014). Morphological variations of the vallate papillae in some mammalian species. Anatomical science international, 89, 161-170.
• Erdoğan, S., Villar Arias, S., & Pérez, W. (2016). Morphofunctional structure of the lingual papillae in three species of South American Camelids: alpaca, guanaco, and llama. Microscopy research and technique, 79(2), 61-71.
• Erdoğan, S., & Sağsöz, H. (2018). Papillary architecture and functional characterization of mucosubstances in the sheep tongue. The Anatomical Record, 301(8), 1320-1335.
• Goodarzi, N., & Hoseini, T. S. (2015). Fine structure of lingual papillae in the Markhoz goat (Iranian Angora): a scanning electron microscopic study. International Journal of Zoological Research, 11(4), 160-168.
• Goździewska‐Harłajczuk, K., Klećkowska‐Nawrot, J., Janeczek, M., & Zawadzki, M. (2015). Morphology of the lingual and buccal papillae in alpaca (Vicugna pacos)–Light and scanning electron microscopy. Anatomia, histologia, embryologia, 44(5), 345-360.
• Harem, İ. Ş., Sarı, E.K., & Harem, M.K. (2009). Akkaraman Koyununda Dorsal Dil Papillalarının Işık ve Taramalı Elektron Mikroskopik Yapısı. Kocatepe Veterinary Journal, 2(2), 8-14.
• Harem, M. K., Harem, I. S., Sari, E. K., & Aydin, M. F. (2011). Light and scanning electron microscopic study of the dorsal lingual papillae of the Goitered gazelle (Gazelle subgutturosa). Journal of Animal and Veterinary Advances, 10(15), 1906-1913.
• Kokubun, H. S., Zanatta Esper, G. V., Rezende Franciolli, A. L., de Oliveira e Silva, F. M., Rici, R .E. G., & Miglino, M. A. (2012). Estudo histológico e comparativo das papilas linguais dos cervídeos Mazama Americana e Mazama gouzoubira por microscopia de luz e eletrônica de varredura. Pesq Vet Bras, 32(10), 1061–1066.
• Kumar, P., Kumar, S., & Singh, Y. (1998). Tongue papillae in goat: a scanning electron‐microscopic study. Anatomia, Histologia, Embryologia, 27(6), 355-357.
• Kurtul, I., & Atalgın, S. H. (2008). Scanning electron microscopic study on the structure of the lingual papillae of the Saanen goat. Small Ruminant Research, 80(1-3), 52-56.
• Sari, E. K., Harem, M. K., & Harem, I. S. (2010). Characteristics of dorsal lingual papillae of Zavot cattle. Journal of animal and veterinary advances, 9(1), 123-130.
• Shao B, Long R, Ding Y, Wang J, Ding L, & Wang H. 2010. Morphological adaptations of yak (Bos grunniens) tongue to the foraging environment of the Qinghai-Tibetan plateau. J Anim Sci, 88, 2594–2603.
• Tabata, S., Wada, A., Kobayashi, T., Nishimura, S., Muguruma, M., & Iwamoto, H. (2003). Bovine circumvallate taste buds: Taste cell structure and immunoreactivity to α‐gustducin. The Anatomical Record, 271(1), 217- 224.
• Türkyılmaz, D., (2014). The effect of growth hormone (GH) gene polymophisms on some production and placental properties in Morkaraman sheep. PHD thesis, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Zootekni Anabilim Dalı, Erzurum.
• Toprak, B., Candan, S., & Koçakoğlu, N. Ö. (2020). Ankara Keçisinde (Capra hircus) Dil Papillalarının Işık ve Taramalı Elektron Mikroskobik Yapısı Üzerine İncelemeler: I. Tat Papillaları. Etlik Veteriner Mikrobiyoloji Dergisi, 31(2), 158-166.
• Roth G, & Wake D. 1989. Conservatism and innovation in the evolution of feeding in vertebrates. In: DB Wake, G Roth, editors. Complex organismal functions: integration and evolution in vertebrates. New York: John Wiley & Sons. p 7–21.
• Yoshimura K, Hama N, Shindo J, Kobayaski K, & Kageyama I. (2009). Light and scanning electron microscopic study on the tongue and lingual papillae of the hippopotamus, Hippopotamus amphibious. Anatomical Record, 292, 921-934.