Stereological Examination of Parietal and Temporal Regions of the Brain in Rats Induced with Epilepsy with Pentylenetetrazole

Year 2024, Volume: 14 Issue: 2, 187 - 200, 25.12.2024

Veysel Akyol , Gamze Çakmak

https://doi.org/10.53518/mjavl.1520971

Abstract

The aim of this study was to investigate the parietal and temporal regions of the brain stereologically after epilepsy induced by pentylenetetrazole (PTZ). 20 healthy male Wistar Albino rats, 3 months old, weighing approximately 250 gr, were chosen for the study. The rats were divided into PTZ and control groups, 10 in each group. 80 mg/kg PTZ was mixed into 0.5 ml of 0.9% physiological saline and administered intraperitoneally as a single dose injection to the PTZ group. Behavioral changes specified in six stages were determined by following the Racine scale within a 30-minute observation period. After perfusion, the rats’ brains were removed. After tissue tracking, an average of 10 sections were obtained for each animal, with a thickness of 1/75 of 5µm. Sections were stained with Hematoxylin-Eosin. The preparations were photographed with a 1.25 objective. For each brain tissue, the total lobus parietalis, lobus temporalis, the grey matter volume and the total number of neurons in the lobus parietalis and lobus temporalis were calculated. Mann-Whitney U test was used for statistical analysis (IBM SPSS for Windows, ver.25) No statistically significant difference could be detected in the total lobus parietalis, lobus temporalis and the grey matter volume, volume values of the brain and the number of neurons determined between the groups (p>0.05). However, when evaluated according to groups, a significant difference was obtained in the volume ratio values, the grey matter volume/total lobus parietalis and the grey matter volume/total lobus temporalis ratio (p<0.05).

Keywords

Epilepsy, Parietal lobe, Pentylenetetrazole (PTZ), Stereology, Temporal lobe.

Ethical Statement

This study was conducted at Experimental Medicine Application and Research Center, in accordance with the decision of Van Yuzuncu Yil University Animal Experiments Local Ethics Committee dated 28.11.2019 and numbered 2019/11. There was no tampering with the data collected and this study was not sent to any other academic publication environment for evaluation.

Supporting Institution

Van Yuzuncu Yil University Scientific Research Projects Directorate

Project Number

TDK-2020-8840

Thanks

We would thank to Van Yuzuncu Yil University Scientific Research Projects Directorate

Pentilentetrazol ile Epilepsi Oluşturulan Sıçanlarda Beynin Parietal ve Temporal Bölgelerinin Stereolojik İncelenmesi

Abstract

Bu çalışmanın amacı, pentilentetrazol (PTZ) ile oluşturulan epilepsi sonrası beynin pariyetal ve temporal bölgelerini stereolojik olarak incelemektir. Çalışma için yaklaşık 250 gr ağırlığında, 3 aylık, 20 adet sağlıklı erkek Wistar Albino sıçan seçildi. Sıçanlar her grupta 10 adet olacak şekilde PTZ ve kontrol gruplarına ayrıldı. 80 mg/kg PTZ, 0.5 ml %0.9 fizyolojik salin içerisine karıştırıldı ve PTZ grubuna tek doz enjeksiyon halinde intraperitoneal olarak uygulandı. Altı aşamada belirlenen davranış değişiklikleri, 30 dakikalık gözlem süresi içerisinde Racine skalası takip edilerek belirlendi. Perfüzyonun ardından sıçanların beyinleri çıkarıldı. Doku takibi sonrasında her hayvan için 1/75 5 µm kalınlığında ortalama 10 adet kesit elde edildi. Kesitler Hematoksilen-Eozin ile boyanarak 1.25 objektifle fotoğraflandı. Her beyin dokusu için lobus parietalis, lobus temporalis, gri madde hacmi ve lobus parietalis ve lobus temporalis'teki toplam nöron sayısı hesaplandı. İstatistiksel analizde Mann-Whitney U testi kullanıldı (IBM SPSS for Windows, ver.25).Lobus parietalis, lobus temporalis ve beyindeki gri madde hacmi, hacim değerleri ve gruplar arasında nöron sayısı değerlerinde istatistiksel olarak anlamlı bir fark saptanmadı. (p>0.05). Ancak gruplara göre değerlendirildiğinde hacim oranı değerlerinde yani gri madde hacmi/toplam lobus parietalis ve gri madde hacmi/toplam lobus temporalis oranında anlamlı farklılık elde edildi (p<0.05).

Keywords

Epilepsi, Parietal lob, Pentylenetetrazol (PTZ), Stereoloji, Temporal lob.

Project Number

TDK-2020-8840

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