Melatonin Improves Postoperative Cognitive Dysfunction in Aged Rats: Relevance of Oxidative Stress, PSD95 and Ca2+/Calmoduline Dependent Protein Kinase

Year 2023, , 225 - 233, 31.08.2023

İnci Turan , Veysel Haktan Özaçmak , Hale Sayan Özaçmak

https://doi.org/10.29058/mjwbs.1342979

Abstract

Aim: Postoperative cognitive dysfunction (POCD) is usually seen in the aged patients in postoperative
period. In the present study, we aimed to investigate the effects of melatonin on cognitive and synaptic
dysfunction and oxidative stress in POCD model constructed by abdominal surgery in aged rats.
Material and Methods: Twenty months old male Wistar rats were randomly allocated into the control
group, melatonin treated-control group, surgery group and melatonin treated-surgery group. The novel
object recognition test (NORT) was used to assess the postoperative cognitive ability of rats at the
end of the experiments. The contents of malondialdehyde (MDA) and reduced glutathione (GSH) were
evaluated in the synaptosomes of hippocampus by spectrophotometric methods. The protein levels of
calcium-calmodulin dependent protein kinase II (CaMKII) and postsynaptic density protein 95 (PSD95)
were measured by using ELISA. .
Results: Animals in surgery group showed significant an impairment of novel object recognition memory
in NORT. Surgery group also exhibited increased content of MDA (21.05±3.96) and decreased levels
of GSH (5.86±0.26) and PSD95 (156.04±2.56) in the hippocampus (respectively p=0.012, p<0.001,
p=0.004) . The levels of PSD95 (193.08±4.14) and CaMKII (2.82±0.25) (respectively p=0.027, p=0.041)
and novel object recognition memory was improved by melatonin treatment.
Conclusion: Melatonin may be a potential therapeutic agent which can protect against abdominal
surgery- induced cognitive dysfunction in elderly patients.

Keywords

Postoperative cognitive dysfunction, melatonin, oxidative stress, PSD95, CaMKII

Project Number

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Melatonin Yaşlı Sıçanlarda Postoperatif Kognitif Disfonksiyonu İyileştirir: Oksidatif Stres, PSD95 ve Ca2+/Kalmodulin Bağımlı Protein Kinazın Önemi

Abstract

Amaç: Postoperatif kognitif disfonksiyon (POCD) genellikle postoperatif dönemde yaşlı hastalarda
görülmektedir. Bu çalışmada, yaşlı sıçanlarda abdominal cerrahi ile oluşturulan POCD modelinde
melatoninin kognitif ve sinaptik disfonksiyon ve oksidatif stres üzerindeki etkilerini araştırmayı amaçladık.
Gereç ve Yöntemler: Yirmi aylık erkek Wistar sıçanlar rastgele olarak kontrol grubu, melatonin
uygulanan-kontrol grubu, cerrahi grubu ve melatonin uygulanan-cerrahi grubuna ayrıldı. Deneylerin
sonunda sıçanların ameliyat sonrası bilişsel yeteneklerini değerlendirmek için yeni obje tanıma testi
(NORT) kullanıldı. Hipokampüs sinaptozomlarında malondialdehit (MDA) ve indirgenmiş glutatyon
(GSH) içerikleri spektrofotometrik yöntemlerle ölçüldü. kalsiyum-kalmodulin bağımlı protein kinaz II
(CaMKII) ve postsinaptik dansite protein-95 (PSD95) protein seviyeleri ELISA kullanılarak ölçüldü.
Bulgular: Cerrahi grubundaki hayvanlar NORT’ta yeni obje tanıma belleğinde önemli bir bozulma
göstermiştir. Ayrıca cerrahi grubunda hipokampüste MDA içeriğinde (21,05±3,96) artış ve GSH (5,86±0,26) ve PSD95 (156,04±2,56) seviyelerinde azalma görülmüştür (sırasıyla p=0,012, p<0,001, p=0,004). PSD95 (193,08±4,14) ve
CaMKII (2,82±0,25) düzeyleri (sırasıyla p=0,027, p=0,041) ve yeni obje tanıma belleği melatonin uygulaması ile iyileşmiştir.
Sonuç: Melatonin, yaşlı hastalarda abdominal cerrahinin neden olduğu bilişsel işlev bozukluğuna karşı koruma sağlayabilecek potansiyel
bir terapötik ajan olabilir.

Keywords

Postoperatif kognitif disfonksiyon, melatonin, oksidatif stres, PSD95, CaMKII

Supporting Institution

Yok

Project Number

Yok

Thanks

Yok

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