Histological Scoring Systems for the Assessment of the Degree of Lung Injury in Rats

Abstract

Acute respiratory distress syndrome (ARDS) is a serious pulmonary reaction with well-defined clinical parameters in humans triggered by many causes besides bacterial and viral pneumonia. However, there is no definitive definition of ARDS parameters in the experimental animal model. With its 2010 workshop report, the American Thoracic Society defined the main histopathological features that determine the presence of ARDS in laboratory animals, such as changes in parenchymal tissue, altered integrity of the alveolar capillary barrier, inflammation, and abnormal lung function. Understanding these parameters, scoring tissue lesions is used to convert observational pathological data into semi-quantitative or quantitative data for statistical analysis and improved precision.

Keywords

• Rat
• ARDS
• ALI
• Histopathological grading
• lung

Sıçanlarda Akciğer Hasarının Değerlendirilmesi için Kullanılan Histolojik Derecelendirme Sistemleri

Abstract

Akut solunum sıkıntısı sendromu (ARDS), bakteriyel ve viral pnömoninin yanı sıra birçok nedenin tetiklediği, insanlarda klinik parametrelerin çok iyi tanımlanmış ciddi bir akciğer reaksiyonudur. Ancak deneysel hayvan modelinde ARDS parametrelerine ilişkin kesin bir tanımlama mevcut değildir. Amerikan Toraks Derneği 2010 çalıştay raporuyla laboratuvar hayvanlarında ARDS varlığını belirleyen parankimal dokuda değişiklikler, alveoler-kapiller bariyerin bütünlüğünün değişmesi, iltihaplanma ve anormal akciğer fonksiyonu gibi histopatolojik ana özellikler tanımlamıştır. Bu parametreleri anlamak ve gözlemsel patolojik verileri istatistiksel analiz ve gelişmiş kesinlik için yarı niceliksel veya niceliksel verilere dönüştürmek için doku lezyonlarının skorlanması yöntemi kullanılmaktadır.

Keywords

• Sıçan
• ARDS
• ALI
• Histopatolojik derecelendirme
• Akciğer

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