Effect of Thyroid Hormone Pre-conditioning on Intestinal and Lung Damage in The Intestinal Ischemia/Reperfusion Model

Abstract

Pre-conditioning of 1-3,3 ', 5-triiodothronine (T3) or 1-tyroxine (T4) in various experimental studies has been shown to be protective against hepatic, brain, cardiac and renal ischemia reperfusion (I/R) damage. Intestinal I/R injury, a critical condition usually caused by many clinical scenarios such as shock, acute mesenteric ischemia, sepsis, mesenteric thrombosis, or bowel transplantation. The purpose of this study is to examine the possible protective effect of preconditioning with T3 against damage to the small intestine and lung tissue caused by intestinal I / R. Male Wistar Albino rats were subjected to mesenteric ischemia (30 min) followed by reperfusion (3h). Four groups were designed, each of contain 8 animals: sham control; I/R control; I/R +50µg/kg T3 administration; and I/R+100µg/kg T3 administration. Intestinal and lung tissue damage was examined histopathologically. Our results indicate that 100 µg/kg T3 preconditioning attenuates the I/R-induced histopathological changes in intestinal and lung tissues. Histopathological damage assessment in both lung and intestinal tissue was found to be high in the I/R group. In the 100µg T3 treated group, intestinal mucosa and lung injury were found to be lower than I / R and 50µg T3 treated groups. In conclusion, it was observed that T3-induced preconditioning before intestinal I/R injury was histopathologically protective effect on small intestine and lung tissue caused by intesinal ischemia.

Keywords

• Thyroid Hormone,Intesinal Ischemia,Lung Injury,Reperfusion

İntestinal İskemi/Reperfüzyon Modelinde Tiroid Hormon Ön Koşullanmasının İnce Bağırsak ve Akciğer Hasarı Üzerine Etkisi

Abstract

Çeşitli deneysel çalışmalarda l-3,3′,5- triiodotronin (T3) veya ltiroksin (T4) önkoşullanmasının karaciğer, beyin, kalp ve böbrek iskemi reperfüzyon (İ/R) hasarına karşı koruyucu etkili olduğu gösterilmiştir. İntestinal I/R hasarı, genellikle şok, akut mezenterik iskemi, sepsis, mezenterik tromboz veya bağırsak nakli gibi birçok klinik durumun neden olduğu kritik bir tablodur. Bu çalışmanın amacı T3 ile oluşturulan önkoşullanmanın intestinal İ/R ile oluşan ince bağırsak ve akciğer dokusundaki hasara karşı olası koruyucu etkisini incelemektir. Erkek Wistar Albino cinsi sıçanlara 30 dakika mezenterik iskemi ve sonrasında 3 saat reperfüzyon uygulandı. Her grupta 8 hayvan olmak üzere 4 gruba ayrıldılar: sham kontrol, İ/R kontrol, İ/R +50µg/kg T3 uygulanan ve İ/R+100µg/kg T3 uygulanan grup. İntestinal ve akciğer dokusundaki hasar histopatolojik olarak incelenmiştir. Sonuçlarımız ince bağırsak ve akciğer dokusunda 100 µg/kg dozunda T3 önkoşullanmasının İ/R ile oluşan patolojik değişikleri azalttığını göstermektedir. İ/R grubunda hem akciğer hem de intestinal dokuda histopatolojik hasar değerlendirmesinin yüksek seviyesinde olduğu bulunmuştur. 100 µg İ/R +T3 uygulanan grupta intestinal mukoza ve akciğer hasarının İ/R ve İ/R +50µg T3 uygulanan gruba göre düşük olduğu saptanmıştır. Sonuç olarak, intestinal I/R hasarından önce T3 ile oluşturulan önkoşullanmanın bağırsak iskemisinin neden olduğu ince bağırsak ve akciğer dokusu üzerinde histopatolojik olarak koruyucu etkili olduğu gözlemlendi.

Keywords

• Tiroid Hormon,İntestinal İskemi,Akciğer Hasarı,Reperfüzyon

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