Çevresel Zenginleştirme Bilateral Karotid Arter Oklüzyonu ile Oluşan Retinal Oksidatif Stresi Azaltır

Abstract

Amaç: Çevresel zenginleştirmenin iskemiye veya travmaya bağlı gelişen patolojilerde hasarı azalttığı çeşitli çalışmalar tarafından gösterilmiştir. Bilateral karotid arter oklüzyonu (2VO) kapiller damar hasarına, özellikle optik sinir ve retinada dejenerasyona neden olmaktadır. Bu çalışmanın amacı çevresel zenginleştirmenin 2VO yapılan sıçanlarda retinal oksidatif stres ve C vitamini üzerine etkisini incelemektir.

Gereç ve Yöntemler:  Çalışmada 24 adet Wistar albino erkek sıçan (250-300 g)  kullanıldı. Denekler rasgele olmak üzere kontrol, 2VO+standart çevre (N), ve 2VO+zenginleştirilmiş çevre (EE) olarak 3 gruba ayrıldı. 2VO kalıcı bilateral karotid arter oklüzyonu ile gerçekleştirildi ve EE 4 hafta boyunca uygulandı. Deney sonunda göz dokularında malondialdehid (MDA), indirgenmiş glutatyon (GSH) ve askorbik asit düzeyleri ölçüldü.

Bulgular: Çevresel zenginleştirme standart çevre grubu ile karşılaştırıldığında retinal MDA seviyelerini azalttı. 2VO göz dokusunda, kontrol grubu ile karşılaştırıldığında GSH düzeylerini ve C vitamini miktarını düşürdü. (p<0.05). EE standart çevre grubuna kıyasla GSH seviyelerini düzeltti. Fakat EE’nin retinal askorbik asit düzeylerini etkilemediği gözlendi (p>0.05).

Sonuç: Bu çalışmanın sonuçları çevresel zenginleştirmenin 2VO ile retinada oluşan oksidatif stresi azaltmada etkili olabileceğini göstermektedir.

Keywords

• Çevresel zenginleştirme,Karotid arter oklüzyonu,Oksidatif stres,Retina

Environmental Enrichment Attenuate Retinal Oxidative Stress Induced by Bilateral Carotid Artery Occlusion

Abstract

 Aim: Various studies have shown that environmental enrichment reduces the tissue damage in ischemia or trauma-related pathologies. Bilateral carotid artery occlusion (2VO) leads to capillary vessel damage, especially degeneration of the optic nerve and retina. The aim of this study was to investigate the effect of environmental enrichment on retinal oxidative stress and vitamin C in 2VO rats. 

Material and Methods: 24 Wistar Albino male rats (250-300 g) were used in the study. The rats were randomly separated into 3 groups as the control, 2VO+ standart environment (SE), 2VO+ environmental enrichment (EE). 2VO was induced by permanent bilateral carotid artery occlusion and EE was performed for 4 weeks. At the end of this period malondialdehyde (MDA), reduced glutathione (GSH) and ascorbic acid levels were measured in eye tissues.  

Results: Environmental enrichment reduced retinal MDA levels compared with the standard environmental group. 2VO decreased GSH levels and vitamin C compared with control group in eye tissues. EE ameliorated GSH levels compared to standard environmental group. However, EE did not affect retinal ascorbic acid levels. 

Conclusion: The results of this study show that environmental enrichment may be effective in reducing 2VO induced oxidative stress in the retina. 

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