İskemi Sonrası Verilen Kannabidiol Reperfüzyon ile Uyarılan Aritmilere Karşı Koruyucu Etkilidir

Year 2022, Volume: 6 Issue: 1, 16 - 23, 28.04.2022

Salih Erdem Ersöz Gonca Günselin Başoğlu Elifnur Aydemir

https://doi.org/10.29058/mjwbs.1016783

Abstract

Amaç: Kannabidiol (KBD) psikolojik etkileri bulunmayan bir bitkisel kannabinoiddir. Klinik uygulamada
Huntington hastalığı ve ultipl skleroz tedavisinde kullanılmaktadır. Sıçanlarda koroner arter ligasyonu
öncesi verilen KBD’nin reperfüzyon ile uyarılan ventriküler aritmilere karşı koruyucu etkili olduğu
bildirilmiştir. Ancak reperfüzyon aritmilerinin klinik tedavisinde kullanılabilmesi için miyokardiyal iskemi
sonrasında verildiğinde de etkili olduğu gösterilmelidir. Bu çalışmanın amacı sıçanlarda miyokardiyal
iskemi ve reperfüzyon periyotları boyunca infüzyon yolu ile uygulanan KBD tedavisinin reperfüzyon
aritmilerine olan etkilerini araştırmaktır.
Gereç ve Yöntemler: Miyokardiyal iskemi ve reperfüzyon, anestezi uygulanmış erkek sıçanlarda
sırasıyla sol ana koroner arterin 6 dakika ligasyonu ve damar üzerindeki ligatürün 6 dakika açılmasıyla
reperfüzyon gerçekleştirildi. Çalışma üç ayrı deney grubunda yürütüldü; (1) Kontrol (n= 6), (2) Lig-KBD
(n=6), (3) Rep-KBD (n=6). KBD infüzyon ile sırasıyla Lig-KBD grubunda ligasyon ve Rep-KBD grubunda
reperfüzyon periyodu boyunca 9 μg/90 μL/dk/kg dozda intravenöz yolla verildi.
Bulgular: Ligasyon boyunca KBD tedavisi ventriküler prematüre kasılma (VPK) süresini kontrol grubuna
göre anlamlı azalttı (p=0,024). Reperfüzyonda KBD tedavisi, aritmi skoru, ventriküler taşikardi (VT),
ventriküler prematüre kasılma (VPK) ve toplam aritmi sürelerini kontrol grubuna göre azalttı (Toplam
aritmi süresi: kontrol; 38,19(6,29-72,44) sn, Lig-KBD; 8,69(0-34,9) sn, Rep-KBD; 1,49(0,14-4,87)sn,
p=0,007).
Sonuç: Bu çalışmanın sonuçları reperfüzyon periyodu boyunca verilen KBD’nin reperfüzyon ile uyarılan
aritmilere karşı güçlü antiaritmik etkiye sahip olduğunu göstermektedir. KBD reperfüzyon aritmilerinin
klinik tedavisinde kullanılabilme potansiyeline sahiptir.

Keywords

Kannabidiol, Reperfüzyon aritmileri, Sıçan, İnfüzyon

Supporting Institution

Zonguldak Bülent Ecevit Üniversitesi

Project Number

2016-84906727-04

Cannabidiol Given Following the Ischemia Confers Protection Against Reperfusion-Induced Arrhythmia

Abstract

Aim: Cannabidiol (CBD) is a non-psychoactive plant cannabinoid. In clinical practice, it has been used
in the treatment of Huntington’s disease and multiple sclerosis. CBD given before the coronary ligation
has been reported to be protective against reperfusion-induced arrhythmia in rats. However, it should
also be demonstrated that is effective against ventricular arrhythmias during ischemia and reperfusion
in order to be adapted to the clinical treatment of reperfusion arrhythmias. The aim of this study is to
investigate the effects of CBD applied by infusion during both ischemia and reperfusion periods on
reperfusion arrhythmias in rats.
Material and Methods: Myocardial ischemia and reperfusion were accomplished by the ligation of the
left main coronary artery for 6 min and release of the tie on the vessel for 6 min in male anesthetized grurats,
respectively. CBD was administered by infusion pump at a dose of 9 μg/90 μL/min/kg during the ligation in Lig-CBD group in Rep-CBD
group reperfusion, respectively. Experimental groups of the study were: (1) Control (n = 6), (2) Lig-CBD (n = 6), and (3) Rep-CBD (n = 6).
Results: CBD treatment during the ligation significantly decreased the duration of ventricular premature contraction (VPC) compared to
the control group (p=0.024). CBD that was given during the reperfusion period significantly decreased the ventricular premature contraction
(VPC), ventricular tachycardia (VT) and total length of arrhythmias compared to the control group (total length of arrhythmias: control:
38.19(6.29-72.44) sec, Lig-CBD; 8.69(0-34.9) sec, Rep-CBD; 1.49(0.14-4.87) sec, p=0.007).
Conclusion: The results of the study indicate that CBD given during the reperfusion period has a strong antiarrhythmic effect on reperfusioninduced
arrhythmia. These results strengthen the potential use of CBD in the clinical treatment of reperfusion arrhythmias.

Keywords

Cannabidiol, Reperfusion arrhythmias, Rat, Infusion

Project Number

2016-84906727-04

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