Research Article
BibTex RIS Cite

In vitro koşullarda farklı dozlarda bor uygulamalarının mersin (Myrtus communis L.) bitkisinin biyokimyasal özellikleri üzerine etkileri

Year 2023, , 46 - 58, 07.04.2023
https://doi.org/10.37908/mkutbd.1153620

Abstract

Araştırmada siyah meyveli bir mahalli çeşit olan ‘tatlı mersin’ genotipinin sürgün uçları materyal olarak kullanılmıştır. Bu çalışma ile ortama eklenen bor konsantrasyonlarından toksisite problemi yaratabilecek bor (B) uygulamaları ile olası B stresi altında mersin bitkisinin morfolojik ve biyokimyasal özelliklerdeki değişimleri incelenmiştir. Bu amaçla 6 farklı borik asit (H3BO3) dozu (12.4 mg L-1, 18.6 mg L-1, 24.8 mg L-1, 31 mg L-1, 37.2 mg L-1, 43.4 mg L-1) Murashige ve Skoog (MS) ortamına eklenmiş ve kontrol grubu ile birlikte bitkilerin gelişimleri takip edilmiştir. Araştırmada kontrol ortamındaki bitkilere yalnızca MS ortam içeriğindeki standart B miktarı (6.2 mg L-1) ilave edilmiştir. Araştırmada, en yüksek prolin miktarı 43.4 mg L-1 uygulamasında 11.6 µg ml-1 olarak elde edilirken, en düşük prolin miktarı kontrol grubunda 3.3 µg ml-1 olarak belirlenmiştir. Askorbat peroksidaz (APX), süperoksit dismutaz (SOD), peroksidaz (POD) enzim aktiviteleri uygulama dozları arttıkça yükselmiş, en düşük değer kontrol grubundaki bitkilerde belirlenmiştir. Sonuç olarak; yetiştirme ortamına eklenen H3BO3 miktarı arttıkça özellikle askorbat peroksidaz, katalaz, süperoksit dismutaz ve peroksidaz gibi stres mekanizmasında rol alan enzimatik antioksidan aktivitelerinde önemli derecede artış gözlenmiştir.

References

  • Acar YS, İşkil R, Erden Y (2018) Bor stresi altında Arabidopsis thaliana (L.) Heynh’da süperoksit dismutaz genlerinin ekspresyon profillerinin belirlenmesi. Journal of Boron, 3(3): 145-150.
  • Akula R, Ravishankar GA (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior, 6(11): 1720- 1731.
  • Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24(1): 1.
  • Aydın C, Özcan MM (2007) Determination of nutritional and physical properties of myrtle (Myrtus communis L.) fruits growing wild in Turkey. Journal of Food Engineering, 79(2): 453-458.
  • Bacelar EA, Santos DL, Moutinho-Pereira JM, Gonçalves BC, Ferreira HF, Correia CM (2006) Immediate responses and adaptative strategies of three olive cultivars under contrasting water availability regimes: changes on structure and chemical composition of foliage and oxidative damage. Plant Science, 170(3): 596-605.
  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1): 205-207.
  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44(1): 276-287.
  • Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry, 195(1): 133-140.
  • Berger KC (1949) Boron in soils and crops. Advances in Agronomy, 1: 321-351.
  • Brdar-Jokanović M (2020) Boron toxicity and deficiency in agricultural plants. International Journal of Molecular Sciences, 21(4): 14-24.
  • Cervilla LM, Blasco B, Ríos JJ, Romero L, Ruiz, JM (2007) Oxidative stress and antioxidants in tomato (Solanum lycopersicum) plants subjected to boron toxicity. Annals of Botany, 100(4): 747-756.
  • Dallı E (2022) Arabidopsis thaliana’da tuz ön uygulaması ile bor tolerans kazanımında antosiyaninin çoklu fonksiyonel rollerinin araştırılması. Yüksek Lisans Tezi, Hacettepe Üniversitesi, Lisansüstü Eğitim Enstitüsü, Biyoloji ABD, 101 s.
  • Dankov K, Busheva M, Stefanov D, Apostolova EL (2009) Relationship between the degree of carotenoid depletion and function of the photosynthetic apparatus. Journal of Photochemistry and Photobiology B: Biology, 96(1): 49-56.
  • Dawson FA (1994) The Amazing Terpenes. Naval Stores Rev. MarchlAprll, 6-12.
  • Eraslan F, Inal A, Gunes A, Alpaslan M (2007) Boron toxicity alters nitrate reductase activity, proline accumulation, membrane permeability, and mineral constituents of tomato and pepper plants. Journal of Plant Nutrition, 30(6): 981-994.
  • Eraydın, E. (2000). Topraklarda Bor Adsorpsiyonu Üzerine Bazı Anyonların Etkisi. Yüksek Lisans Tezi, Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Toprak ABD, 70 s.
  • Erlaçin S, Erciyas E (1978) Myrtus communis L. (Mersin Bitkisi) yapraklarının tanen yönünden incelenmesi. Doğa Bilim Dergisi, 2(1): 75-79.
  • Giansoldati V, Tassi E, Morelli E, Gabellieri E, Pedron F, Barbafieri M (2012) Nitrogen fertilizer improves boron phytoextraction by Brassica juncea grown in contaminated sediments and alleviates plant stress. Chemosphere, 87(10): 1119-1125.
  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Journal of Plant Biochemistry & Physiology, 48: 909–930.
  • Gong H, Zhu X, Chen K, Wang S Zhang C (2005) Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Science, 169: 313-321.
  • Han S, Tang N, Jiang HX, Yang LT, Li Y, Chen LS (2009) CO2 assimilation, photosystem II photochemistry, carbohydrate metabolism and antioxidant system of citrus leaves in response to boron stress. Plant Science, 176(1): 143-153.
  • Hartree EF (1972) Determination of protein: a modification of the lowry method that gives a linear photometric response. Analytical Biochemistry, 48(2): 422-427.
  • Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, Ahmad A (2012) Role of proline under changing environments: a review. Plant Signaling & Behavior, 7(11): 1456-1466.
  • Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207(4), 604-611.
  • Hua T, Zhang R, Sun H, Liu C (2021) Alleviation of boron toxicity in plants: Mechanisms and approaches. Critical Reviews in Environmental Science and Technology, 51(24), 2975-3015.
  • Jiang T, Jahangir MM, Jiang Z, Lu X, Ying T (2010) Influence of UV-C Treatment On Antioxidant Capacity, Antioxidant Enzyme Activity And Texture of Postharvest Shiitake (Lentinus edodes) Mushrooms During Storage. Postharvest Biology and Technology, 56(3), 209-215.
  • Kayıhan DS, Kayıhan C, Çiftçi YÖ (2016) Excess boron responsive regulations of antioxidative mechanism at physio-biochemical and molecular levels in Arabidopsis thaliana. Plant Physiology and Biochemistry, 109: 337-345.
  • Khalid M, Bilal M, Huang DF (2019) Role of flavonoids in plant interactions with the environment and against human pathogens—A review. Journal of Integrative Agriculture, 18(1): 211-230.
  • Knörzer OC, Lederer B, Durner J, Böger P (1999) Antioxidative defense activation in soybean cells. Physiologia Plantarum, 107(3): 294-302.
  • Kosová K, Vítámvás P, Prášilová P, Prášil IT (2013) Accumulation of WCS120 and DHN5 proteins in differently frost-tolerant wheat and barley cultivars grown under a broad temperature scale. Biologia Plantarum, 57(1): 105-112.
  • Kumaran A, Joel Karunakaran R (2006) Antioxidant activities of the methanol extract of Cardiospermum halicacabum. Pharmaceutical Biology, 44(2): 146-151.
  • Landi M, Guidi L, Pardossi A, Tattini M, Gould KS (2014) Photoprotection by foliar anthocyanins mitigates effects of boron toxicity in sweet basil (Ocimum basilicum). Planta, 240(5): 941-953.
  • Landi M, Pardossi A, Remorini D, Guidi L (2013) Antioxidant and photosynthetic response of a purple-leaved and a green-leaved cultivar of sweet basil (Ocimum basilicum) to boron excess. Environmental and Experimental Botany, 85: 64-75.
  • Landi M, Tattini M, Gould KS (2015) Multiple functional roles of anthocyanins in plant-environment interactions. Environmental and Experimental Botany, 119: 4-17.
  • Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11: 591-592.
  • Molassiotis A, Sotiropoulos T, Tanou G, Diamantidis G, Therios I (2006) Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM 9 (Malus domestica Borkh). Environmental and Experimental Botany, 56(1): 54-62.
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
  • Nakano H, Yamauchı J, Hashımoto S (1981) Sunflower spiral antenna. Institute of Electronics, Information and Communication Engineers Transactions, 64(12): 763-769.
  • Oğur R (1994) Mersin bitkisi (Myrtus communis L.) hakkında bir inceleme. Çevre Dergisi, 10: 21–25.
  • Pardossi A, Romani M, Carmassi G, Guidi L, Landi M, Incrocci L, Maggini R, Puccinelli M, Vacca W Ziliani M (2015) Boron accumulation and tolerance in sweet basil (Ocimum basilicum L.) with green or purple leaves. Plant and Soil, 395(1): 375-389.
  • Polidoros AN, Scandalios JG (1999) Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S‐transferase gene expression in maize (Zea mays L.). Physiologia Plantarum, 106(1): 112-120.
  • Rani C, Sharma PK, Kumar B, Angrish R, Datta KS (2008) Alleviation of boron-salt toxicity by calcium in wheat through associated changes in antioxidant defense system. Indian Journal of Plant Physiology, 13(1): 21-28.
  • Sairam RK, Tyagi A (2004) Physiology and molecular biology of salinity stress tolerance in plants. Current Science, 86(3): 407-421.
  • Serce S, Ercisli S, Sengul M, Gunduz K, Orhan E (2010) Antioxidant activities and fatty acid composition of wild grown myrtle (Myrtus communis L.) fruits. Pharmacognosy magazine, 6(21): 9.
  • Sergiev I, Alexieva V, Karanov E (1997) Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Comptes Rendus de l'Academie Bulgare des Sciences, 51(3): 121-124.
  • Singleton VL, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3): 144-158.
  • Soy M, Güneş A (2003) Fosforun domates (Lycopersicon esculentum L.) bitkisinde bor toksisitesini önlemede etkisi. Tarım Bilimleri Dergisi, 9(3): 273-277.
  • Stetsenko LA, Pashkovsky PP, Voloshin RA, Kreslavski VD, Kuznetsov VLV, Allakhverdiev SI (2020) Role of anthocyanin and carotenoids in the adaptation of the photosynthetic apparatus of purple-and green-leaved cultivars of sweet basil (Ocimum basilicum) to high-intensity light. Photosynthetica, 58(4): 890-901.
  • Vuran NE, Türker M (2021) Bitki doku kültürlerinde sekonder metabolit miktarını arttırmaya yönelik uygulamalar. International Journal of Advances in Engineering and Pure Sciences, 33(3): 487-498.
  • Wilkinson RE (1994) Plant-Environment Interactions. Marcel Dekker Inc., New York.
  • Wink M (2009) Introduction. In Annual Plant Reviews, Functions and Biotechnology of Plant Secondary Metabolites. pp 1-20.
  • Yeğin AB, Halil Uzun (2015) Mersin (Myrtus communis L.) meyvelerinin fenolik bileşik içerikleri. Derim, 32(1): 81-88.
  • Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64(4): 555-559.
  • Zinkel DF, Russell J (1989) Naval Stores: Production, Chemistry, Utilization. (New York: Pulp Chemicals Association).
  • Zlatev ZS, Lidon FC, Ramalho JC, Yordanov IT (2006) Comparison of resistance to drought of three bean cultivars. Biologia Plantarum, 50(3): 389-394.

The effects of boron applications with different doses on the biochemical properties of myrtle plant (Myrtus communis L.) in in vitro conditions

Year 2023, , 46 - 58, 07.04.2023
https://doi.org/10.37908/mkutbd.1153620

Abstract

In this study, shoot tips of the 'sweet myrtle' genotype which is a local variety with black fruits, were used as study material. It was aimed to determine the level of boron (B) that may cause toxicity problems from six different B concentrations added to the growing media and the changes in biochemical properties of myrtle plants under possible boron stress were examined. Six different boric acid (H3BO3) doses (12.4 mg L-1, 18.6 mg L-1, 24.8 mg L-1, 31 mg L-1, 37.2 mg L-1, 43.4 mg L-1) was added to the Murashige and Skoog (MS) medium and the development of the plants was compared with the control group. Only standard B content (6.2 mg L-1) was added in the MS medium to the plants in the control group. As a result of examining the statistical evaluations; the highest proline amount was obtained as 11.6 µg ml-1 in 43.4 mg L-1 application in the media which has the highest H3BO3 concentration, whereas the lowest proline amount was determined as 3.3 µg ml-1 in the control group. Ascorbat peroxidase, superoxide dismutase, peroxidase enzyme activities increased as the application doses increased and the lowest value was determined in the plants in the control group. As the amount of B added to the growing medium increased, a significant increase was observed in the enzymatic antioxidant activities that play a role in the stress mechanism, especially ascorbat peroxidase, catalase, superoxide dismutase, peroxidase. As a result of the study, it was determined that the tolerance limits of the myrtle plant against boron stress were high.

References

  • Acar YS, İşkil R, Erden Y (2018) Bor stresi altında Arabidopsis thaliana (L.) Heynh’da süperoksit dismutaz genlerinin ekspresyon profillerinin belirlenmesi. Journal of Boron, 3(3): 145-150.
  • Akula R, Ravishankar GA (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior, 6(11): 1720- 1731.
  • Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24(1): 1.
  • Aydın C, Özcan MM (2007) Determination of nutritional and physical properties of myrtle (Myrtus communis L.) fruits growing wild in Turkey. Journal of Food Engineering, 79(2): 453-458.
  • Bacelar EA, Santos DL, Moutinho-Pereira JM, Gonçalves BC, Ferreira HF, Correia CM (2006) Immediate responses and adaptative strategies of three olive cultivars under contrasting water availability regimes: changes on structure and chemical composition of foliage and oxidative damage. Plant Science, 170(3): 596-605.
  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1): 205-207.
  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44(1): 276-287.
  • Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry, 195(1): 133-140.
  • Berger KC (1949) Boron in soils and crops. Advances in Agronomy, 1: 321-351.
  • Brdar-Jokanović M (2020) Boron toxicity and deficiency in agricultural plants. International Journal of Molecular Sciences, 21(4): 14-24.
  • Cervilla LM, Blasco B, Ríos JJ, Romero L, Ruiz, JM (2007) Oxidative stress and antioxidants in tomato (Solanum lycopersicum) plants subjected to boron toxicity. Annals of Botany, 100(4): 747-756.
  • Dallı E (2022) Arabidopsis thaliana’da tuz ön uygulaması ile bor tolerans kazanımında antosiyaninin çoklu fonksiyonel rollerinin araştırılması. Yüksek Lisans Tezi, Hacettepe Üniversitesi, Lisansüstü Eğitim Enstitüsü, Biyoloji ABD, 101 s.
  • Dankov K, Busheva M, Stefanov D, Apostolova EL (2009) Relationship between the degree of carotenoid depletion and function of the photosynthetic apparatus. Journal of Photochemistry and Photobiology B: Biology, 96(1): 49-56.
  • Dawson FA (1994) The Amazing Terpenes. Naval Stores Rev. MarchlAprll, 6-12.
  • Eraslan F, Inal A, Gunes A, Alpaslan M (2007) Boron toxicity alters nitrate reductase activity, proline accumulation, membrane permeability, and mineral constituents of tomato and pepper plants. Journal of Plant Nutrition, 30(6): 981-994.
  • Eraydın, E. (2000). Topraklarda Bor Adsorpsiyonu Üzerine Bazı Anyonların Etkisi. Yüksek Lisans Tezi, Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Toprak ABD, 70 s.
  • Erlaçin S, Erciyas E (1978) Myrtus communis L. (Mersin Bitkisi) yapraklarının tanen yönünden incelenmesi. Doğa Bilim Dergisi, 2(1): 75-79.
  • Giansoldati V, Tassi E, Morelli E, Gabellieri E, Pedron F, Barbafieri M (2012) Nitrogen fertilizer improves boron phytoextraction by Brassica juncea grown in contaminated sediments and alleviates plant stress. Chemosphere, 87(10): 1119-1125.
  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Journal of Plant Biochemistry & Physiology, 48: 909–930.
  • Gong H, Zhu X, Chen K, Wang S Zhang C (2005) Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Science, 169: 313-321.
  • Han S, Tang N, Jiang HX, Yang LT, Li Y, Chen LS (2009) CO2 assimilation, photosystem II photochemistry, carbohydrate metabolism and antioxidant system of citrus leaves in response to boron stress. Plant Science, 176(1): 143-153.
  • Hartree EF (1972) Determination of protein: a modification of the lowry method that gives a linear photometric response. Analytical Biochemistry, 48(2): 422-427.
  • Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, Ahmad A (2012) Role of proline under changing environments: a review. Plant Signaling & Behavior, 7(11): 1456-1466.
  • Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207(4), 604-611.
  • Hua T, Zhang R, Sun H, Liu C (2021) Alleviation of boron toxicity in plants: Mechanisms and approaches. Critical Reviews in Environmental Science and Technology, 51(24), 2975-3015.
  • Jiang T, Jahangir MM, Jiang Z, Lu X, Ying T (2010) Influence of UV-C Treatment On Antioxidant Capacity, Antioxidant Enzyme Activity And Texture of Postharvest Shiitake (Lentinus edodes) Mushrooms During Storage. Postharvest Biology and Technology, 56(3), 209-215.
  • Kayıhan DS, Kayıhan C, Çiftçi YÖ (2016) Excess boron responsive regulations of antioxidative mechanism at physio-biochemical and molecular levels in Arabidopsis thaliana. Plant Physiology and Biochemistry, 109: 337-345.
  • Khalid M, Bilal M, Huang DF (2019) Role of flavonoids in plant interactions with the environment and against human pathogens—A review. Journal of Integrative Agriculture, 18(1): 211-230.
  • Knörzer OC, Lederer B, Durner J, Böger P (1999) Antioxidative defense activation in soybean cells. Physiologia Plantarum, 107(3): 294-302.
  • Kosová K, Vítámvás P, Prášilová P, Prášil IT (2013) Accumulation of WCS120 and DHN5 proteins in differently frost-tolerant wheat and barley cultivars grown under a broad temperature scale. Biologia Plantarum, 57(1): 105-112.
  • Kumaran A, Joel Karunakaran R (2006) Antioxidant activities of the methanol extract of Cardiospermum halicacabum. Pharmaceutical Biology, 44(2): 146-151.
  • Landi M, Guidi L, Pardossi A, Tattini M, Gould KS (2014) Photoprotection by foliar anthocyanins mitigates effects of boron toxicity in sweet basil (Ocimum basilicum). Planta, 240(5): 941-953.
  • Landi M, Pardossi A, Remorini D, Guidi L (2013) Antioxidant and photosynthetic response of a purple-leaved and a green-leaved cultivar of sweet basil (Ocimum basilicum) to boron excess. Environmental and Experimental Botany, 85: 64-75.
  • Landi M, Tattini M, Gould KS (2015) Multiple functional roles of anthocyanins in plant-environment interactions. Environmental and Experimental Botany, 119: 4-17.
  • Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11: 591-592.
  • Molassiotis A, Sotiropoulos T, Tanou G, Diamantidis G, Therios I (2006) Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM 9 (Malus domestica Borkh). Environmental and Experimental Botany, 56(1): 54-62.
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
  • Nakano H, Yamauchı J, Hashımoto S (1981) Sunflower spiral antenna. Institute of Electronics, Information and Communication Engineers Transactions, 64(12): 763-769.
  • Oğur R (1994) Mersin bitkisi (Myrtus communis L.) hakkında bir inceleme. Çevre Dergisi, 10: 21–25.
  • Pardossi A, Romani M, Carmassi G, Guidi L, Landi M, Incrocci L, Maggini R, Puccinelli M, Vacca W Ziliani M (2015) Boron accumulation and tolerance in sweet basil (Ocimum basilicum L.) with green or purple leaves. Plant and Soil, 395(1): 375-389.
  • Polidoros AN, Scandalios JG (1999) Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S‐transferase gene expression in maize (Zea mays L.). Physiologia Plantarum, 106(1): 112-120.
  • Rani C, Sharma PK, Kumar B, Angrish R, Datta KS (2008) Alleviation of boron-salt toxicity by calcium in wheat through associated changes in antioxidant defense system. Indian Journal of Plant Physiology, 13(1): 21-28.
  • Sairam RK, Tyagi A (2004) Physiology and molecular biology of salinity stress tolerance in plants. Current Science, 86(3): 407-421.
  • Serce S, Ercisli S, Sengul M, Gunduz K, Orhan E (2010) Antioxidant activities and fatty acid composition of wild grown myrtle (Myrtus communis L.) fruits. Pharmacognosy magazine, 6(21): 9.
  • Sergiev I, Alexieva V, Karanov E (1997) Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Comptes Rendus de l'Academie Bulgare des Sciences, 51(3): 121-124.
  • Singleton VL, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3): 144-158.
  • Soy M, Güneş A (2003) Fosforun domates (Lycopersicon esculentum L.) bitkisinde bor toksisitesini önlemede etkisi. Tarım Bilimleri Dergisi, 9(3): 273-277.
  • Stetsenko LA, Pashkovsky PP, Voloshin RA, Kreslavski VD, Kuznetsov VLV, Allakhverdiev SI (2020) Role of anthocyanin and carotenoids in the adaptation of the photosynthetic apparatus of purple-and green-leaved cultivars of sweet basil (Ocimum basilicum) to high-intensity light. Photosynthetica, 58(4): 890-901.
  • Vuran NE, Türker M (2021) Bitki doku kültürlerinde sekonder metabolit miktarını arttırmaya yönelik uygulamalar. International Journal of Advances in Engineering and Pure Sciences, 33(3): 487-498.
  • Wilkinson RE (1994) Plant-Environment Interactions. Marcel Dekker Inc., New York.
  • Wink M (2009) Introduction. In Annual Plant Reviews, Functions and Biotechnology of Plant Secondary Metabolites. pp 1-20.
  • Yeğin AB, Halil Uzun (2015) Mersin (Myrtus communis L.) meyvelerinin fenolik bileşik içerikleri. Derim, 32(1): 81-88.
  • Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64(4): 555-559.
  • Zinkel DF, Russell J (1989) Naval Stores: Production, Chemistry, Utilization. (New York: Pulp Chemicals Association).
  • Zlatev ZS, Lidon FC, Ramalho JC, Yordanov IT (2006) Comparison of resistance to drought of three bean cultivars. Biologia Plantarum, 50(3): 389-394.
There are 55 citations in total.

Details

Primary Language Turkish
Subjects Agricultural Engineering
Journal Section Araştırma Makalesi
Authors

Cansu Dindar 0000-0002-0592-7513

Adnan Yıldırım 0000-0003-2526-040X

Civan Çelik 0000-0002-1696-5902

Publication Date April 7, 2023
Submission Date August 3, 2022
Acceptance Date September 28, 2022
Published in Issue Year 2023

Cite

APA Dindar, C., Yıldırım, A., & Çelik, C. (2023). In vitro koşullarda farklı dozlarda bor uygulamalarının mersin (Myrtus communis L.) bitkisinin biyokimyasal özellikleri üzerine etkileri. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 28(1), 46-58. https://doi.org/10.37908/mkutbd.1153620

22740137731737513771 13774 15432 1813713775 14624 15016 i2or 1857924881download