The effect of silicon applications on seedling growth stress parameters in lettuce (Lactuca sativa L.) under salt stress conditions

Abstract

Salt stress prevents plant growth and development by disrupting osmotic and ionic balance. Inthisstudy; As a result of silicon (Si) application, which can activate tolerance mechanism sagainst salt stress in lettuce seedlings; Changes in antioxidant (SOD, CAT, APX, POD) and physiological parameters (proline, leaf chlorophyllratio, leaf membrane permeability and relative water content) effective on seedling growth were investigated. Control plants grew beter than plants treated with only salt. As a result of Si+100 mM NaCl and Si+50 mM NaCl applications; Increases between 176% and 339% in SOD, CAT, APX, POD levels, increases between 26.9% and 28.1% in leaf chlorophyll and moisture content and decreases between 15.2% and 76.4% in leaf membrane damage were determined. The relationship between proline accumulation and antioxidant enzyme activities was effective with Si application and showed that plants improved their defense against salt stress. Plant development decreased according to the severity of salt as a result of only salt applications. Itwasdeterminedthat I50x100kg.ha-1Si interaction was moreeffective on plantgrowth in allplots.

Keywords

• lettuce seedling
• potassium silicate
• NaCl
• tolerance

Tuz stresi koşullarında marulda (Lactuca sativa L.) silikon uygulamalarının fide büyüme stresi parametrelerine etkisi

Öz

Tuz stresi bitkilerin büyümesini ve gelişmesini osmotik ve iyon dengesini bozarak engellemektedir. Bu çalışmada; Marul fidelerinde tuz stresine karşı tolerans mekanizmalarını harekete geçirebilen silikon (Si) uygulaması sonucunda; Fide büyümesinde etkili olan antioksidan (SOD, CAT, APX, POD) ve fizyolojik parametrelerdeki (prolin, yaprak klorofil oranı, yaprak membran geçirgenliği ve bağıl su içeriği) değişimler araştırılmıştır. Kontrol bitkileri, yalnızca tuzla muamele edilen bitkilerden daha iyi büyüdü. Si+100 mM NaCl ve Si+50 mM NaCl uygulamaları sonucunda; SOD, CAT, APX, POD seviyelerinde %176 ile %339 arasında artışlar, yaprak klorofili ve nem içeriğinde %26,9 ile %28,1 arasında artışlar ve yaprak zarı hasarında %15,2 ile %76,4 arasında azalmalar belirlendi. Prolin birikimi ile antioksidan enzim aktiviteleri arasındaki ilişki Si uygulamasıyla etkili olmuş ve bitkilerin tuz stresine karşı savunmayı geliştirdiklerini göstermiştir. Sadece tuz uygulamaları sonucunda tuzun şiddetine göre bitki gelişimi azalmıştır. I50x100kg.ha-1Si etkileşiminin tüm parsellerde bitki büyümesinde daha etkili olduğu belirlendi.

Anahtar Kelimeler

• marul fidesi
• potasyum silicat
• NaCl
• tölerans

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