Determination of fungal and bacterial microbiota of broomrape species found in their major host plants grown in Hatay province of Türkiye

Abstract

The aim of this study was to determine the fungal and bacterial microbiomes of broomrape species (Orobanche ramosa and O. crenata) encountered in their major host crops in different districts of Hatay province of Türkiye. Fungal isolates obtained from plants showing disease symptoms were identified as Rhizoctonia solani, Fusarium incarnatum, Sclerotinia sclerotiorum, Alternaria alternata, Epicoccum nigrum, Aspergillus niger and Rhizopus oryzae as a result of morphological, MALDI-TOF MS and molecular identification studies. Antagonist/plant growth promoting bacterial species such as Bacillus cereus, Bacillus megaterium, Bacillus mycoides, Bacillus weihenstephanensis, Bacillus pumilus, Bacillus putida, Bacillus simplex, Enterobacter cloacae, Glutamicibacter mysorens, Lysinibacillus sphaericus, Pseudomonas cedrina ssp. cedrina, Pseudomonas chlororaphis, Pseudomonas pseudomycoides, Pseudomonas trivialis, Pseudomonas thivervalensis, Pseudomonas umsongensis, Rhizobium radiobacter, Solibacillus silvestris, Stenotrophomonas maltophilia and Variovorax paradoxus were isolated from healthy broomrape plants and pathogenic bacterial species such as Pseudomonas cichorii, Pseudomonas corrugata, Pseudomonas marginalis and Pseudomonas mediterranea were isolated from plant samples of broomrape plants showing symptoms of disease and identified by MALDI-TOF MS analysis.

Keywords

• Orobanche spp.
• MALDI TOF
• Bacterial agents
• Fungal Agents

Türkiye’nin Hatay ilinde yetiştirilen önemli tarımsal ürünlerde karşılaşılan canavar otu türlerinin fungal ve bakteriyel mikrobiyotasının belirlenmesi

Öz

Bu çalışmada, Türkiye’nin Hatay ilinin farklı ilçelerinde yetiştiriciliği yapılan önemli tarımsal öneme sahip kültür bitkilerinde karşılaşılan canavar otu türlerinin (Orobanche ramosa ve O. crenata) fungal ve bakteriyel mikrobiyomlarının belirlenmesi amaçlanmıştır. Hastalık belirtisi gösteren bitkilerden yapılan izolasyonlarda elde edilen fungal izolatlar morfolojik, MALDI-TOF MS ve moleküler tanılama çalışmaları sonucunda Rhizoctonia solani, Fusarium incarnatum, Sclerotinia sclerotiorum, Alternaria alternata, Epicoccum nigrum, Aspergillus niger ve Rhizopus oryzae olarak teşhis edilmiştir. Sağlıklı canavar otu bitki örneklerinden Bacillus cereus, Bacillus megaterium, Bacillus mycoides, Bacillus weihenstephanensis, Bacillus pumilus, Bacillus putida, Bacillus simplex, Enterobacter cloacae, Glutamicibacter mysorens, Lysinibacillus sphaericus, Pseudomonas cedrina ssp. cedrina, Pseudomonas chlororaphis, Pseudomonas pseudomycoides, Pseudomonas trivialis, Pseudomonas thivervalensis, Pseudomonas umsongensis, Rhizobium radiobacter, Solibacillus silvestris, Stenotrophomonas maltophilia ve Variovorax paradoxus gibi antagonist/bitki gelişimini teşvik eden bakteri türleri, hastalık belirtileri gösteren canavar otu bitki örneklerinden ise Pseudomonas cichorii, Pseudomonas corrugata, Pseudomonas marginalis ve Pseudomonas mediterranea gibi patojen karakterli bakteri türleri izole edilerek MALDI-TOF MS ile tanılanmışlardır.

Anahtar Kelimeler

• Orobanche spp
• MALDI TOF
• Fungal etmenler
• Bakteriyel etmenler

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