Effects of beneficial rhizobacteria on the growth parameters of pepper (Capsicum annuum L.)

Abstract

This study aimed to determine the impact of soil-borne rhizobacteria on pepper plant growth. A total of 104 bacterial isolates were obtained from 30 soil samples collected from various production sites across the provinces of Çorum, Tokat, and Yozgat. These isolates were subjected to growth tests at 37 °C, a hypersensitivity reaction test on tobacco, and a pectolytic activity test on potatoes. Based on these evaluations, identification of the 91 selected isolates was determined along with their phosphorus-solubilising and nitrogen-fixing properties. Species-level identification of the bacterial isolates was performed using MALDI-TOF MS analysis. Experiments were conducted in a climate chamber and a greenhouse using the seed application and seedling dipping methods with ten bacterial isolates selected to represent the entire collection. Bacterial suspensions prepared from these isolates were applied to pepper seeds and seedlings, and the effects on the growth of the developing plants were evaluated. These evaluations showed that the bacterial isolates had a positive effect on plant growth parameters. Application of the Pseudomonas thivervalensis (MBB-2) isolate to seeds resulted in a 52.38% increase in plant height during the seedling stage. The most effective bacterial isolate was Pseudomonas corrugata (MBB-6), which was applied to seedlings and resulted in a 34.45% increase in root development and a 40.46% increase in the number of leaves. In conclusion, these findings suggest that these bacteria can significantly promote pepper plant development and have the potential to be used as biofertilisers in agricultural production.

Keywords

• Plant Growth Promoting Rhizobacteria
• Pepper
• Seed
• Seedling

Faydalı rizobakterilerin biber (Capsicum annuum L.) bitkisinin gelişim parametreleri üzerine etkileri

Abstract

Bu çalışma, toprak kökenli kökbakterilerin biber bitkisinin gelişimi üzerindeki etkilerini belirlemek amacıyla yürütülmüştür. Çorum, Tokat ve Yozgat illerindeki farklı üretim alanlarından alınan 30 toprak örneğinden toplam 104 bakteri izolatı elde edilmiştir. İzolatlar; 37 °C’de gelişim, tütünde aşırı duyarlılık reaksiyonu ve patateste pektolitik aktivite testlerine tabi tutulmuş, bu değerlendirmeler sonucunda seçilen 91 izolatın fosforu çözme ve azotu bağlama özellikleri belirlenmiştir. Bakteri izolatlarının tür düzeyindeki tanılamaları MALDI-TOF MS analizi ile gerçekleştirilmiştir. Çalışmada, tüm izolatları temsilen seçilen 10 bakteri izolatı ile tohum uygulaması ve fide daldırma yöntemleri kullanılarak iklim odası ve sera koşullarında denemeler kurulmuştur. İzolatlardan hazırlanan bakteri süspansiyonları biber tohumlarına ve fidelerine uygulanmış, bakterilerin gelişen bitkilerin büyümesi üzerindeki etkileri değerlendirilmiştir. Yapılan değerlendirmeler sonucunda, bakteri izolatlarının bitki gelişim parametreleri üzerinde olumlu etkiler oluşturduğu belirlenmiştir. Tohuma uygulanan Pseudomonas thivervalensis (MBB-2) izolatı, fide döneminde bitki boyunda %52.38 oranında artış sağlamıştır. Fidelere uygulanan Pseudomonas corrugata (MBB-6) izolatı ise kök gelişiminde %34.45 ve yaprak sayısında %40.46 artış ile en etkili bakteri izolatı olarak saptanmıştır. Sonuç olarak, elde edilen bulgular bu bakterilerin biber bitkisinin gelişimini önemli ölçüde teşvik ettiğini ve biyogübre olarak tarımsal üretimde kullanılma potansiyeline sahip olduğunu göstermiştir.

Keywords

• Bitki Gelişimini Teşvik Eden Rizobakteriler
• Biber
• Tohum
• Fide

Supporting Institution

Destekleyen kurum yoktur.

Ethical Statement

Bu makalede insan veya hayvan deneklerle herhangi bir çalışma bulunmaması nedeniyle etik onaya gerek duyulmamaktadır.

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