Rhyzopertha dominica erginlerinin Beauveria bassiana konidisporları uygulanan buğday tanelerine davranışsal tepkisi ve ölüm oranı

Abstract

Bu çalışma, zararlı hareketi ve ölüm oranı belirlenerek Rhyzopertha dominica erginlerinin Beauveria bassiana sporu uygulanmış buğday tanelerine olan tepkisini incelemek amacıyla yürütülmüştür. Zararlının inaktive edilmiş B. bassiana sporlarına tepkileri de ayrıca test edilmiştir. Her deney ünitesi R. dominica erginlerinin bölmeler arasında geçişinin kısıtlanmadığı iki eşit bölmeden oluşmuştur. Bölmelere spor uygulanmış veya spor uygulanmamış buğday taneleri doldurularak farklı düzenekler oluşturulmuştur. Fungus uygulamalarında aktif veya inaktive edilmiş B. bassiana konidileri kullanılmıştır. R. dominica ergin salımlarının sadece bir bölmeye veya her iki bölmeye yapıldığı iki deneme kurulmuştur. Deneme sonunda böceklerin dağılımı ve ölüm oranları kaydedilmiştir. R. dominica erginleri, B. bassiana sporlarının varlığına karşı belirgin bir kaçınma davranışı sergilemiş ve toplam ölüm oranı %95,00'ten %68,75'e düşmüştür. R. dominica erginleri inaktive edilmiş B. bassiana sporlarına da aynı tepkiyi göstermiş olup aktif ve inaktif sporlar arasında ayrım yapmamışlardır. B. bassiana uygulamasının başarısına zararlı davranışının etkisi kullanım stratejisine bağlı olarak değişebilir: temiz alanlarda toplanma nedeniyle homojen olmayan dağılımlar etkinlikte düşüşe neden olabilecekken, fungusun repellent özelliği zararlının bulaşmasını engelleme açısından avantaj sağlayabilecektir.

Keywords

• Böcek davranışı
• Kaçınma
• Biyolojik mücadele
• Entomopatojen fungus

Behavioral response of Rhyzopertha dominica adults to wheat kernels treated with Beauveria bassiana conidia and mortality rate

Abstract

This study was conducted to investigate the reaction of Rhyzopertha dominica adults to treated-wheat kernels with Beauveria bassiana spores considering pest movement and mortality. The pest’s reaction to inactivated B. bassiana spores was also tested. Each experimental unit had two equal-sized of compartments without restriction of R. dominica adult movements to both sites. By filling each compartment with wheat kernels treated or untreated, different layouts were created using two compartments. Treatments included either active or inactivated B. bassiana conidia. In setup of two experiments, R. dominica adults were released in either one or both compartments. The distribution of insects and mortalities were recorded at the end of the experiments. R. dominica adults exhibited a marked avoidance behavior towards the presence of B. bassiana spores, reducing the total mortality from 95.00% to 68.75%. R. dominica adults showed the same response to inactivated B. bassiana spores, and could not differentiate between active and inactive spores. The effect of this behavior on the success of B. bassiana treatments depends on the application strategy: while non-homogeneous distribution may lead to reduced efficacy due to insects aggregating in untreated areas, the fungus's inherent repellency offers a strategic advantage for preventing initial infestations.

Keywords

• Insect behavior
• Avoidance
• Biological control
• Entomopathogenic fungi

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