Determination of the host range of Pectobacterium polaris causing bacterial soft rot disease

Year 2022, Volume: 27 Issue: 2, 234 - 240, 01.08.2022

Murat Ozturk

https://doi.org/10.37908/mkutbd.1064147

Cited By: 1

Abstract

Aims: Pectobacterium polaris is a novel species of Pectobacterium taxa, which was reported in different regions of the world and has been recently detected in Turkey. In this study, in order to determine the host range of this novel species, two representative strains were evaluated in terms of their pathogenicity on several host plants, and it is aimed to determine plant species under the risk of the pathogen.

Methods and Results: P. polaris strains NV3 and NV19, isolated from potato plants showing blackleg symptoms in Nevşehir province during the vegetation period of 2019, were used for artificial inoculation. Bacterial suspension of both strains was inoculated by injecting into the red cabbage, broccoli, cauliflower, onion, garlic, carrot, tomato, pepper, eggplant, cucumber, squash, melon, watermelon, lettuce, sunflower and sugar beet plants. The appearance of the disease symptoms indicated that both strains of P. polaris caused disease in all the plant species inoculated artificially. Viable bacterial colonies were re-isolated from infected plant tissue.

Conclusions: Pectobacterium species are very destructive bacterial plant pathogens that cause soft rot on numerous plant species. It has been determined that P. polaris, which has been reported in different regions of the world and in Turkey as a new emerging pathogen within in a wide host range. Disease protection precautions should be taken in the regions where the pathogen is isolated for the first time, and especially pathogen transmission should be prevented to the clean production areas by potato tubers.

Significance and Impact of the Study: All tested plant species were determined as potential hosts for P. polaris. The risk status of the pathogen on these plants, which have economic importance in Turkey, was determined for the first time with this study

Keywords

Bacterial disease, Pectobacterium polaris, host range, pathogenicity

Supporting Institution

Yozgat Bozok University

Project Number

6602c-ZF/21-472

Thanks

This study was supported by financial grant of project management office, Yozgat Bozok University, project no 6602c-ZF/21-472. The author would like to sincerely thank Prof. Dr. Soner SOYLU for valuable comments and important criticisms, and Ahmet DEMİRCİ for diligent proofreading on the manuscript

Bakteriyel yumuşak çürüklük hastalığına neden olan Pectobacterium polaris’in konukçu aralığının belirlenmesi

Abstract

Amaç: Pectobacterium polaris, dünyanın farklı bölgelerinde bildirilen ve yakın zamanda Türkiye’de tespit edilen Pectobacterium spp.’de yer alan yeni bir bakteriyel patojendir. Bu çalışmada P. polaris’in konukçu aralığını belirlemek için, patojenin iki ayrı izolatının farklı konukçularda patojenisitesini değerlendirmek ve etmene karşı risk altında olabilecek bitki türlerinin belirlenmesi hedeflenmiştir.

Yöntem ve Bulgular: Nevşehir ilinde karabacak hastalık belirtisi gösteren patates bitkilerinin enfekteli gövde dokusundan 2019 yılı vejetasyon döneminde izole edilmiş P. polaris (NV3 and NV19) izolatları bitkilere yapay inokulasyonlar için kullanılmıştır. İzolatların bakteriyel süspansiyonları hazırlanarak kırmızı lahana, brokoli, karnabahar, soğan, sarımsak, havuç, domates, biber, patlıcan, hıyar, kabak, kavun, karpuz, marul, ayçiçeği ve şeker pancarı bitkilerine bulaştırılmıştır. Yapay inokulasyon yapılan tüm bitkilerde meydana gelen hastalık simptomları ile P. polaris’in her iki izolatının hastalığa neden olduğu belirlenmiştir. Enfekteli bitki dokularından patojene ait koloniler yeniden izole edilmiştir.

Genel Yorum: Pectobacterium türleri, çok sayıda bitki türünde yumuşak çürüklüğe neden olan oldukça yıkıcı bakteriyel bitki patojenleridir. Dünyanın farklı bölgelerinde bildirilen ve ülkemizde oldukça yeni bir patojen olarak tanımlanan P. polaris’in geniş bir konukçu aralığında patojen olduğu belirlenmiştir. Patojenin ilk olarak izole edildiği bölgede hastalığın mücadelesine yönelik önlemler alınmalı ve özellikle patates yumruları yoluyla patojenin hastalıktan ari üretim alanlarına taşınması önlenmelidir.

Çalışmanın Önemi ve Etkisi: Testlenen tüm bitkilerin P. polaris için konukçu olduğu belirlenmiştir. Türkiye’de ekonomik öneme sahip olan bu kültür bitkilerinin patojene karşı risk durumları ilk kez bu çalışma ile belirlenmiştir.

Keywords

Bakteriyel hastalık, Pectobacterium polaris, konukçu aralığı, patojenisite.

Project Number

6602c-ZF/21-472

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