Molecular characterization of some parsley (Petroselinum crispum Mill.) genotypes

Year 2023, Volume: 28 Issue: 1, 236 - 244, 07.04.2023

Ömer Faruk Coşkun , Yakup Fevzi Gündüz , Seher Toprak , Kazım Mavi

https://doi.org/10.37908/mkutbd.1216419

Cited By: 1

Abstract

Parsley (Petroselinum crispum Mill.), a vegetable whose leaves are consumed, has many benefits for human health. The first step of parsley breeding is the characterization of existing genotypes. In this study, genetic diversity of 12 different parsley genotypes was determined by ISSR (Inter Simple Sequence Repeat) marker system. In the study, 41 of the 130 bands obtained from 16 ISSR primers were found to be polymorphic. The similarity coefficients ranged from 0.86–0.99 for ISSR. The average polymorphism was 31.5% and the number of bands varied between 4 and 14. While the genotypes that are genetically closest to each other were determined as the 5th and 6th genotypes, the most distant genotypes were the 3rd and 8th genotypes. Findings from the present study showed that there were genetic variations among the parsley genotypes examined. The obtained data will enable more effective utilization of the parsley genotypes, the genetic differences of which have been determined for the future breeding programs.

Keywords

Petroselinum crispum, Parsley, ISSR, Molecular characterization

Supporting Institution

TÜBİTAK

Thanks

This study is financially supported by TÜBİTAK-BİDEB (2209-A).

Bazı maydanoz (Petroselinum crispum Mill.) genotiplerinin moleküler karakterizasyonu

Abstract

Yaprakları tüketilen bir sebze olan maydanozun (Petroselinum crispum Mill.) insan sağlığına birçok faydası bulunmaktadır. Maydanoz ıslahının ilk adımı mevcut genotiplerin karakterizasyonudur. Bu çalışmada 12 farklı maydanoz genotipinin genetik çeşitliliği ISSR (Inter Simple Sequence Repeat) markır sistemi ile belirlenmiştir. 16 adet ISSR primerinden elde edilen 130 banttan 41’inin polimorfik olduğu tespit edilmiştir. ISSR için benzerlik katsayılarının 0.86–0.99 arasında değiştiği tespit edilmişitr. Ortalama polimorfizm %31.5 olup bant sayısı 4-14 arasında değişmektedir. Genetik olarak birbirine en yakın genotiplerin 5 ve 6 numaralı genotipler olduğu; en uzak genotiplerin ise 3 ve 8 numaralı genotipler olduğu belirlenmiştir. Çalışmada sonuçları, incelenen maydanoz genotipleri arasında genetik varyasyon olduğunu göstermiştir. Elde edilen veriler, gelecekteki ıslah programlarında genetik farklılıkları belirlenen maydanoz genotiplerinin daha etkin kullanılmasını sağlayacaktır.

Keywords

Petroselinum crispum, Maydanoz, ISSR, Moleküler karakterizasyon

References

• Abou El-Leel, O.F., Emam, K.A., & Ibrahim, H.M. (2017) Genetic study, chemical composition and volatile oil of two parsley cultivars (Petroselinum crispum L.) used in botanical gardens and cultivated under Egyptian conditions. Nature and Science, 15 (11), 83-94 https://doi.org/10.1007/978-3-030-38792-1_13
• Abu-Serie, M.M., Habashy, N.H., & Maher, A.M. (2019) In vitro anti-nephrotoxic potential of Ammi visnaga, Petroselinum crispum, Hordeum vulgare, and Cymbopogon schoenanthus seed or leaf extracts by suppressing the necrotic mediators, oxidative stress and inflammation. BMC Complementary Medicine and Therapies 19, 1-16. https://doi.org/10.1186/s12906-019-2559-8
• Agyare, C., Appiah, T., Boakye, Y.D., & Apenteng, J.A. (2017) Chapter 25 – Petroselinum crispum: a Review. Medicinal Spices and Vegetables from Africa, Academic Press, 527-547. https://doi.org/10.1016/B978-0-12-809286-6.00025-X
• Aslan, N., Coskun, O.F., Dalda-Sekerci, A., & Gulsen, O. (2021) Moleküler markörler kullanarak çerezlik kabaklarda (Cucurbita pepo L.) saflık düzeylerinin tahmin edilmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 26 (3), 759-769. https://doi.org/10.37908/mkutbd.995779. In Turkish
• Boutsika, A., Sarrou, E., Cook, C.M., Mellidou, I., Avramidou, E., Angeli, A., Martens, S., Ralli, P., Letsiou, S., Selini, A., Grigoriadis, I., Tourvas, N., Kadoglidou, K., Kalivas, A., Maloupa, E., Xanthopoulou, A., & Ganopoulos, I. (2021) Evaluation of parsley (Petroselinum crispum) germplasm diversity from the Greek Gene Bank using morphological, molecular and metabolic markers. Industrial Crops and Products, 170, 113767. https://doi.org/10.1016/j.indcrop.2021.113767
• Campos, K.E.D., Balbi, A.P.C., & Alves, M.J.Q.D.F. (2009) Diuretic and hipotensive activity of aqueous extract of parsley seeds (Petroselinum sativum Hoffm.) in rats. Revista Brasileira de Farmacognosia, 19, 41-45. https://doi.org/10.1590/S0102-695X2009000100010
• Coskun, O.F., Gülşen, O., Dalda-Şekerci, A., Yetişir, H., & Pinar, H. (2017) Bazı çerezlik kabak hatlarında SSR markır analizi. Akademik Ziraat Dergisi 6, 151-156. In Turkish
• Dice, L.R. (1945) Measures of the amount of ecologic association between species. Ecology, 26, 297-302. https://doi.org/10.2307/1932409
• Domblides, A.S., Domblides, E.A., Kharchenko, V.A., & Potekhin, G.A. (2010) Study of genetic variation among parsley (Petroselinum crispum Mill. Nym.) samples using RAPD and ISSR markers. Moscow University Biological Sciences Bulletin, 65 (4), 152-154. https://doi.org/10.3103/S0096392510040073
• Doyle, J.J., & Doyle, J.L. (1990) Isolation of plant DNA from fresh tissue. Focus, 12, 13-15.
• Evanno, G., Regnaut, S., & Goudet, J. (2005) Detecting the number of clusters of individuals using the software Structure: a simulation study. Molecular Ecology, 14 (8), 2611-2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x
• Ezer, N., & Arisan, O.M. (2006) Folk medicines in Merzifon (Amasya, Turkey). Turkish Journal of Botany, 30, 223-230.
• Falush, D., Stephens, M., & Pritchard, J.K. (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics, 164, 1567-1587. https://doi.org/10.1093/genetics/164.4.1567
• Farzaei, M.H., Abbasabadi, Z., Ardekani, M.R.S., Rahimi, R., & Farzaei, F. (2013) Parsley: a review of ethnopharmacology, phytochemistry and biological activities. Journal of Traditional Chinese Medicine, 33 (6), 815-826. https://doi.org/10.1016/S0254-6272(14)60018-2
• Ganopoulos, I., Merkouropoulos, G., Pantazis, S., Tsipouridis, C., & Tsaftaris, A. (2011) Assessing molecular and morpho-agronomical diversity and identification of ISSR markers associated with fruit traits in quince (Cydonia oblonga). Genetics and Molecular Research, 10, 2729-2746. https://doi.org/10.4238/2011.November.4.7
• Ibrahim, H.M.M., El-Leel, O.F.A., & Emam, K.A. (2017) Molecular profiling for genetic variability in Petroselinum crispum based on ISSR and RAPD markers. Middle East Journal of Agriculture, 6 (1), 67-75. https://doi.org/10.1007/s12033-011-9446-y.
• Karaman K., Dalda-Sekerci A., Yetisir, H., Gulsen, O., & Coskun, O.F. (2018) Molecular, morphological and biochemical characterization of some Turkish bitter melon (Momordica charantia L.) genotypes. Industrial Crops and Products, 123, 93-99. https://doi.org/10.1016/j.indcrop.2018.06.036
• Kırac, H., Dalda-Sekerci, A., Coskun, O.F., & Gulsen, O. (2022) Morphological and molecular characterization of garlic (Allium sativum L.) genotypes sampled from Turkey. Genetic Resources and Crop Evolution, 1-9. https://doi.org/10.1007/s10722-022-01343-4
• Morilipınar, E.O., Dalda-Sekerci, A., Coskun, O.F., & Gulsen, O. (2021) Genetic analysis of local pumpkin populations. International Journal of Agricultural and Natural Sciences, 14 (3), 264-272.
• Nasiri, K., Shojaeiyan, A., Yadollahi, A., Mirshekari, A., & Ghanbari, K. (2015) Genetic diversity assessment of some Iranian parsley (Petroselinum crispum Mill.) accessions using SRAP molecular marker. Journal of Vegetables Sciences, 1 (1), 1-10. https://doi.org/10.22034/IUVS.2015.15346
• Pinar, H., Coskun, O.F., Uysal, E., Gülşen, O., & Yetişir, H. (2017) Yöresel cırgalan biberi genotiplerinin ISSR markırları ile karakterizasyonu. Akademik Ziraat Dergisi, 6, 145-150. In Turkish
• Pritchard, J.K., Stephens, M., & Donnelly, P. (2000) Inference of population structure using multilocus genotype data. Genetics, 155 (2), 945-959. https://doi.org/10.1093/genetics/155.2.945
• Slighoua, M., Mahdi, I., Amrati, F.E., Cristo, F.D., Amaghnouje, A., Grafov, A., Boucetta, N., Bari, A., & Bousta, D. (2021) Assessment of in vivo estrogenic and anti-inflammatory activities of the hydro-ethanolic extract and polyphenolic fraction of parsley (Petroselinum sativum Hoffm.) Journal of Ethnopharmacology, 265, 113290. https://doi.org/10.1016/j.jep.2020.113290
• Staniszewska, I., Dzadz, L., Nowak, K.W., & Zielinska, M. (2021) Evaluation of storage stability of dried powdered coriander, parsley and celery leaves based on the moisture sorption isotherms and glass transition temperature. LWT - Food Science and Technology, 146, 111440. https://doi.org/10.1016/j.lwt.2021.111440
• Tan, A., & İnal, A. (2003) Ege Tarımsal Araştırma Enstitüsü bitki genetik kaynakları çalışmaları. Ege Tarımsal Araştırma Enstitüsü Müdürlüğü Yayın No:112, 13 s. İzmir. In Turkish
• Tecirli, T., Dalda-Şekerci, A., Coskun, O.F., & Gülşen, O. (2018) Morphological and molecular diversity among Heliotropium greuteri samples. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi, 34, 1-7.
• TUIK (2021) www.tuik.gov.tr. Accessed on 7 November 2022. In Turkish
• Wang, Y., Zhang, Y., Hou, M., & Han, W. (2022) Anti-fatigue activity of parsley (Petroselinum crispum) flavonoids via regulation of oxidative stress and gut microbiota in mice. Journal of Functional Foods, 89, 104963. https://doi.org/10.1016/j.jff.2022.104963