Türkiye’de şeker pancarı ekim alanlarından tespit edilen potansiyel biyokontrol ajanı Podospora flexuosa (syn. Cladorrhinum flexuosum)’nın izolasyonu ve tanımlanması

Year 2024, Volume: 29 Issue: 1, 242 - 249, 08.04.2024

Meltem Avan , Rıza Kaya

https://doi.org/10.37908/mkutbd.1385507

Abstract

Şeker pancarı (Beta vulgaris L.) Türkiye’nin Konya ilinde yaygın olarak yetiştirilmekte ve Türkiye şeker pancarı üretiminin yaklaşık üçte biri bu ilden elde edilmektedir. 2015-2017 yıllarında bölgedeki şeker pancarı tarlalarında şiddetli kök çürüklüğü ve kuruma belirtileri gösteren bitkilerden yapılan izolasyonlar sonucunda, farklı fungal türlere ait toprak ve yaprak hastalık etmenleri ile birlikte yapraklardan da yeni bir endofitik fungal izolat elde edilmiştir. Fungal izolat besiyerinde donuk sarı, açık zeytinden açık kahverengiye kadar değişen renklerde, hızlı büyüyen koloni benzeri, esnek konidiyoforlara ve septat dallı hiflere sahiptir. Temsili fungal izolat konukçu bitki yapraklarında hastalık belirtilerine neden olmamıştır. Fungal izolatın moleküler tanımlaması ITS gen bölgesine özgü primerler kullanılarak gerçekleştirilirken, morfolojik ve moleküler özelliklerine göre Podospora flexuosa (syn. Cladorrhinum flexuosum) olarak tanımlanmıştır. P. flexuosa’nın toprakta ve bitki materyallerinde saprofit olarak yaşayan bir biyolojik kontrol ajanı olarak bildirildiğinden, çalışmamızda elde edilen fungal izolatın gelecekte bitki hastalık etmenlerinin biyolojik kontrolünde potansiyel bir biyokontrol ajanı olarak kullanılabileceği düünülmektedir. Bildiğimiz kadarıyla, bu fungal tür Türkiye için ilk kez rapor edilmektedir.

Keywords

Beta vulgaris, Podospora flexuosa, Cladorrhinum flexuosum, Mikobiyota, ITS, Patojenisite, Kayıt, Türkiye

Isolation and identification of Podospora flexuosa (syn. Cladorrhinum flexuosum), a potential biocontrol agent detected in sugar beet cultivation areas in Türkiye

Abstract

Sugar beet (Beta vulgaris L.) is widely cultivated in Konya province of Türkiye and about one third of Türkiye’s sugar beet production is obtained from this province. As a result of the isolations made from plants showing severe root rot and desiccation symptoms in sugar beet fields in the region in 2015-2017, a new endophytic fungal isolate was obtained from leaves along with soil and foliar disease agents belonging to different fungal species. The fungal isolate has dull yellow, light olive to light brown, fast growing colony-like, flexible conidiophores and septate branched hyphae. The representative fungal isolate did not cause disease symptoms on host plant leaves. Molecular identification of the fungal isolate was carried out using primers specific to the ITS gene region, and it was identified as Podospora flexuosa (syn. Cladorrhinum flexuosum) based on morphological and molecular characteristics. Since P. flexuosa is reported to be a biological control agent living as a saprophyte in soil and plant materials, it is thought that the fungal isolate obtained in our study can be used as a potential biocontrol agent in the biological control of plant disease agents in the future. To the best of our knowledge, this fungal species is reported for the first time for Türkiye.

Keywords

Beta vulgaris, Podospora flexuosa, Cladorrhinum flexuosum, Mycobiota, ITS, Pathogenicity, Record, Türkiye

Ethical Statement

All authors have equal contribution The authors declare that they have no conflict of interest

Thanks

The authors would like to thank Prof. Dr. Harun Bayraktar and Asst. Prof. Gülsüm Palacıoğlu, who supported the molecular diagnostic studies of the isolates.

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