SİGARA DUMANININ NEDEN OLDUĞU AKCİĞER İNFLAMASYONUNA KARŞI ALFA-LİNOLENİK ASİTİN KORUYUCU ROLÜ

Abstract

Amaç: Yapılan çalışmalar keten yağında bolca bulunan ve omega 3 yağ asitlerinden biri olan alfa-linolenik asitin antioksidan ve anti inflamatuar etkinliğini göstermiştir. Sigara dumanı birçok zararlı bileşeni içerdiğinden akceğerlerde hasara neden olmaktadır. Bu nedenle alfa-linolenik asitin sigara dumanı maruziyetine bağlı akciğer hasarına karşı koruyucu etkinliği olup olmayacağını planladık.

Gereç ve Yöntem: Bu amaçla ferelere 2 ay boyunca sigara dumanı ve 200 mg/kg alfa-linolenik asit verildi. ve akciğerlerindeki siklooksijenaz-2, fosfolipaz A2 ve indüklenebilir nitrik oksit sentaz (iNOS) enzimleri ELISA yöntemiyle analiz edildi.

Bulgular: Farelere gentamisin uygulaması akciğerlerde siklooksijenaz-2(COX-2), fosfolipaz A2 ve iNOS enzimlerinin ekpresyonlarını arttırdı. Sigara dumanına maruz bırakılan farelere alfa-linolenik asit verilmesi bu enzimlerdeki artışı geri çevirdi.

Sonuç: Çalışmamız sigaranın neden olduğu akciğer hasarına karşı alfa-linolenik asit kullanılmasının yararlı olacağını göstermiştir.

Keywords

• Sigara dumanı,akciğer,alfa-linolenik asit,siklooksijenaz-2,fosfolipaz A2,iNOS

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