Effect of Cigarette Smoke on Rhoa/Rho-Kinase Signalization Pathway in Lung

Abstract

Cigarrette smoke exposure has side effects on lungs, cardiovascular systems and some tissue and organs. Rho/rho-kinase signalization pathway has important role bronchial smooth muscle contractions and cancer development of lung fibroblast. Mechanism of molecular of some side effects due to cigarette smoke exposure has not yet been clearly identified. In the present study we aimed to research effect of cigarette smoke exposure on rho/rho-kinase signalization pathway in lungs. For this propose mice (male age: 8 weeks) separated into two groups as control and smoke exposured. The cigarette smoke application continued 7 days in a week during two mouths. In the end of two mounts, Mice were sacrified by cervical dislocation and their lungs were isolated. Then rhoA and rho-kinase enzymes expression and rho-kinase enzyme activity have been determined. Cigarette smoke exposure caused unregulated rhoA and rho-Kinase enzyme expression and elevated rho-kinase enzyme activity. As a result cigarette smoke  exposure elevated activity of rho/rho-kinase signalization pathway.

Keywords

• Cigarette smoke,rhoA,rho-kinase,lung,mice

Akciğerde Rhoa/Rho-Kinaz Sinyalizasyon Yolu Üzerine Sigara İçmenin Etkisi

Abstract

Sigara dumanına maruziyet akciğerler, kalp-damar sistemi ve bazı doku ve organlar üzerine yan etkilere sahiptir. Rho/rho-kinaz sinyalizasyon yolu bronş düz kas kasılmaları ve akciğer fibroblast kanseri gelişmesi üzerine önemli role sahiptir. Sigara dumanına maruz kalmadan dolayı, bazı yan etkilerin moleküler mekanizması henüz net olarak açıklanamamıştır. Bu çalışmada akciğerlerde rho/rho-kinaz sinyalizasyon yolunun üzerinde sigara dumanı maruziyetinin etkisini araştırmayı amaçladık. Bu amaçla fareleri (erkek, yaş: 8 hafta);  kontrol ve sigara dumanına maruz olarak iki gruba ayırdık. Sigara dumanı uygulaması iki ay boyunca haftada 7 gün şeklinde devam etti. İki ayın sonunda, fareler servikal dislokasyon ile sakrifiye edildi ve akciğerleri izole edildi. Daha sonra RhoA ve Rho-kinaz enzimlerin ekspresyonu ve Rho-kinaz enzimi aktivitesi belirlendi. Sigara dumanı maruziyeti düzensiz RhoA ve rho-kinaz enzimi aktivitesinde artışa neden oldu. Sonuç olarak; Sigara dumanı maruziyeti rho/rho-kinaz sinyalizasyon yolunun aktivitesini arttırdı.

Keywords

• Sigara dumanı,RhoA,rho-kinaz,akciğer,fareler

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