SİGARA DUMANININ NEDEN OLDUĞU AKCİĞER İNFLAMASYONUNA KARŞI ALFA-LİNOLENİK ASİTİN KORUYUCU ROLÜ

Year 2016, Volume: 7 Issue: 28, 31 - 36, 30.12.2016

Halil Mahir Kaplan Figen Doran

https://doi.org/10.17944/mkutfd.287833

Abstract

Amaç: Yapılan çalışmalar keten yağında bolca
bulunan ve omega 3 yağ asitlerinden biri olan alfa-linolenik asitin antioksidan
ve anti inflamatuar etkinliğini göstermiştir. Sigara dumanı birçok zararlı
bileşeni içerdiğinden akceğerlerde hasara neden olmaktadır. Bu nedenle
alfa-linolenik asitin sigara dumanı maruziyetine bağlı akciğer hasarına karşı
koruyucu etkinliği olup olmayacağını planladık.

Gereç ve Yöntem: Bu amaçla ferelere 2 ay
boyunca sigara dumanı ve 200 mg/kg alfa-linolenik asit verildi. ve akciğerlerindeki
siklooksijenaz-2, fosfolipaz A2 ve indüklenebilir nitrik oksit sentaz (iNOS)
enzimleri ELISA yöntemiyle analiz edildi.

Bulgular: Farelere gentamisin
uygulaması akciğerlerde siklooksijenaz-2(COX-2), fosfolipaz A2 ve iNOS
enzimlerinin ekpresyonlarını arttırdı. Sigara dumanına maruz bırakılan farelere
alfa-linolenik asit verilmesi bu enzimlerdeki artışı geri çevirdi.

Sonuç: Çalışmamız sigaranın neden olduğu akciğer
hasarına karşı alfa-linolenik asit kullanılmasının yararlı olacağını
göstermiştir.

Keywords

Sigara dumanı, akciğer, alfa-linolenik asit, siklooksijenaz-2, fosfolipaz A2, iNOS

References

• Barua RS, Ambrose JA, Reynolds LJE, DeVoe MC, Zervas JG, Saha DC, Heavy and light cigarette smokers have similar dysfunction of endothelial vasoregulatory activity. Journal of the American College of Cardiology. 2002;1758–63
• Tell GS, Polak JF, Ward BJ, Kittner SJ, Savage PJ, Robbins J. Relation of smoking with carotid artery wall thickness and stenosis in older adults. The Cardiovascular Health Study. The Cardiovascular Health Study (CHS) Collaborative Research Group. Circulation. 1994;90:2905–08.
• Jonas MA, Oates JA, Ockene JK, Hennekens CH. Statement on smoking and cardiovascular disease for health care professionals. Circulation. 1992;86:1664–69.
• Smith CJ, Fischer TH. Particulate and vapor phase constituents of cigarette mainstream smoke and risk of myocardial infarction. Atherosclerosis. 2001;158:257–67.
• Tsuchiya M., Asada A., Kasahara E., Sato EF, Shindo M., Inoue M. Smoking a single cigarette rapidly reduces combined concentrations of nitrate and nitrite and concentrations of antioxidants in plasma. Circulation 2002;105: 1155-57
• Zhang WZ, Venardos K, Chin-Dusting J, Kaye DM. Adverse effects of cigarette smoke on NO bioavailability: role of arginine metabolism and oxidative stress. Hypertension. 2006;48(2):278-85.
• Guo X, Oldham MJ, Kleinman MT, Phalen RF, Kassab GS. Effect of cigarette smoking on nitric oxide, structural, and mechanical properties of mouse arteries. Am J Physiol Heart Circ Physiol. 2006;291(5):2354-61.
• Li T, Molteni A, Latkovich P, Castellani W, Baybutt RC. Vitamin A depletion induced by cigarette smoke is associated with the development of emphysema in rats. J Nutr. 2003;133(8):2629-34.
• Tanriverdi H, Evrengul H, Kuru O, Tanriverdi S, Seleci D, Enli Y, et al. Cigarette smoking induced oxidative stress may impair endothelial function and coronary blood flow in angiographically normal coronary arteries. Circ J. 2006;70(5):593-99.
• Bucher HC, Hengstler P, Schindler C, et al. N-3 polyunsaturated fatty acids in coronary heart disease: a meta-analysis of randomized controlled trials. Am J Med 2002;112:298–04.
• De Lorgeril M, Salen P, Martin JL, et al. Mediterranean diet, traditional risk factors, and the rate of cardiovascular complications after myocardial infarction: final report of the Lyon diet heart study. Circulation 1999;99:779–85.
• Gerster H. Can adults adequately convert alpha-linolenic acid (18:3n-3) to eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3)? Int J Vitam Nutr Res 1998;68:159–73.
• Ogawa A, Suzuki Y, Aoyama T, Takeuchi H. Dietary alpha-linolenic acid inhibits angiotensin-converting enzyme activity and mRNA expression levels in the aorta of spontaneously hypertensive rats. J Oleo Sci. 2009;58(7):355-60.
• Takeuchi H, Sakurai C, Noda R, Sekine S, Murano Y, Wanaka K et al. Antihypertensive effect and safety of dietary alpha-linolenic acid in subjects with high-normal blood pressure and mild hypertension. J Oleo Sci. 2007;56(7):347-60.
• Alessandri C, Pignatelli P, Loffredo L, Lenti L, Del Ben M, Carnevale R, Perrone A, Ferro D, Angelico F, Violi F. Alpha-linolenic acid-rich wheat germ oil decreases oxidative stress and CD40 ligand in patients with mild hypercholesterolemia. Arterioscler Thromb Vasc Biol. 2006;26(11):2577-78.
• Ren J, Chung SH. Anti-inflammatory effect of alpha-linolenic acid and its mode of action through the inhibition of nitric oxide production and inducible nitric oxide synthase gene expression via NF-kappaB and mitogen-activated protein kinase pathways. J Agric Food Chem. 2007;55(13):5073-80.
• Norum, K.R., Drevon,C.A.. Dietary omega-3 fatty acids and cardiovascular diseases. Arteriosclerozis, 1986; 6: 352-55.
• Bayizit, A. A. Doymamış yağ asitlerinin beslenme ve sağlık açısından önemi. Gıda ve Yem Bilimi -Teknolojisi Dergisi, 2003; 3: 28-31.
• Farrell, D.J. The hearty egg. Poultry Digest, 1992;7: 20-22.
• Farrell, D.J. Une's designer egg. Poult. Int. 1993;32: 62-66.
• Lewis N.M., Seburg, S., Flanagen, N.L. 2000. Enriched eggs as a sourced of n-3 polyunsaturated fatty acids for humans. Poult. Sci. 1993;79: 971-74.
• Leaf, A.,Weber, P.C. Cardiovascular effects of ω-3 fatty acids. The New Engl. J.Of Med. 1988; 318:549-57.
• Cheng SE, Luo SF, Jou MJ, Lin CC, Kou YR, Lee IT, Hsieh HL, Yang CM. Cigarette smoke extract induces cytosolic phospholipase A2 expression via NADPH oxidase, MAPKs, AP-1, and NF-kappaB in human tracheal smooth muscle cells. Free Radic Biol Med. 2009 1;46(7):948-60
• Yang CM, Lee IT, Lin CC, Yang YL, Luo SF, Kou YR, Hsiao LD. Cigarette smoke extract induces COX-2 expression via aPKCalpha/c-Src/EGFR, PDGFR/PI3K/Akt/NF-kappaB pathway and p300 in tracheal smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 2009;297(5):L892-02.
• Tithof PK, Richards SM, Elgayyar MA, Menn FM, Vulava VM, McKay L, et al. Activation of group IVC phospholipase A(2) by polycyclic aromatic hydrocarbons induces apoptosis of human coronary artery endothelial cells. Arch Toxicol. 2011 Jun;85(6):623-34.
• Ozbek E. Induction of Oxidative Stress in Kidney. International Journal of Nephrology. 2012;2012:465897. doi: 10.1155/2012/465897.