Investigation of the Physiological and Histopathological Effects of Omega Acids (3, 6, 9) and Stearic Acid on Rats in Ischemia Reperfusion

Abstract

Ischemia causes reversible or irreversible cell or tissue damage due to insufficient blood flow to the organ or tissue. In this study, our aim is to investigate the protective effect of omega 3, 6 9 and stearic acid application before ischemia reperfusion injury in the leg muscles. For this purpose, 70 female albino rats were divided into 10 groups. The study continued at the same dose for 14 days. In addition, these fatty acids were given to other groups without ischemia-reperfusion. After the application different faty acid, blood biochemical parameters of different fatty acids, oxidative stress parameters and histopathology of tissues (liver, kidney, muscle) were examined in rats. As a result, it was observed that omega 9 fatty acid has better protective properties compared to other omega fatty acids and stearic acid in terms of histopathological properties and oxidative stress index. Additionally, other fatty acids and stearic acid provided some degree of protection against the deleterious effects of ischemia-reperfusion

Keywords

• Ischemia-reperfusion
• total antioxidant status
• total oxidant status
• omega fatty acids
• stearic acid
• oxidative stress

Omega Asitleri (3, 6, 9) ve Stearik Asidin Sıçanlarda İskemi Reperfüzyon Hasarında Fizyolojik ve Histopatolojik Etkilerinin Araştırılması

Abstract

İskemi, organ veya doku kan akımının yetersizliğine bağlı olarak gelişen geri dönüşümlü veya geri dönüşümsüz hücre veya doku hasarına neden olur. Bu çalışmada, sıçanların bacak kaslarında (kuadriseps) oluşturulan iskemi-reperfüzyon hasarı öncesinde sıçanlara aynı dozda verilen farklı yağ asitleri omega 3, 6, 9 ve stearik asit yağ asitlerinin etkinliği araştırıldı. Bu çalışmada farklı yağ asitlerinin koruyucu etkisi; Omega-3, 6, 9 ve stearik asitin, 10 gruptaki (her biri n=7) 70 dişi Wistar-albino sıçan üzerinde iskemiye reperfüzyon hasarına karşı araştırılması. İnsan diyetinde bulunan farklı yağ asitleri (Omega-3, Omega-6, Omega-9 ve stearik asit) sıçanlara bacak kasında (kuadriseps) oluşan iskemi-reperfüzyon hasarından önce 14 gün boyunca aynı dozda uygulandı. Ayrıca bu yağ asitleri iskemi-reperfüzyon işlemi yapılmadan sadece diğer gruplara verildi. Uygulama sonrasında sıçanlarda farklı yağ asitlerinin kan biyokimyasal parametreleri, oksidatif stres parametreleri (toplam antioksidan ve oksidan durum ve oksidatif stres indeksi) ve histopatoloji (kuadriseps kası, karaciğer ve böbrek) açısından etkileri araştırıldı. Sonuçlar, omega 9 yağ asidinin iskemi reperfüzyon hasarına karşı koruyucu etki gösterdiğini göstermektedir. Ayrıca diğer yağ asitleri ve stearik asit, iskemi-reperfüzyonun zararlı etkilerine karşı bir dereceye kadar koruma sağlar. Çalışmamızın sonuçları, uygulanan bu dozda omega 9 yağ asidinin histopatolojik özellikler ve oksidatif stres indeksi üzerine diğer omega asitler ve stearik asit ile karşılaştırıldığında daha iyi koruyucu olduğunu göstermektedir.

Keywords

• İskemi-reperfüzyon
• toplam antioksidan durumu
• toplam oksidan durumu
• omega yağ asitleri
• stearik asit
• oksidatif stres.

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