Phenolic Compound Analysis and Antioxidant Properties of O. Alborosea

Abstract

The Onosma genus has been used as a treatment among the public for a long time. In this study, the content analysis and some biological activities of the plant O. Alborosea Fisch. & C.A.Mey. ssp. (O. alborosea) used in traditional medicine in our country were investigated. For this purpose, methanol and water extracts of the plant were taken and the total phenolic substance amount of each extract was determined by HPLC device. FRAP, CUPRAC reduction methods and DPPH, ABTS radical scavenging methods were used to determine antioxidant activities. According to the results of our study, it was determined that water and methanol extracts showed lower activity compared to standard antioxidants. It can be said that the reason for this is related to the ratio of the phenolic amount they contain. Since no antioxidant studies were found when the literature search related to the current plant species was conducted, it is thought that our study will guide other studies to be carried out with this plant species and is important in terms of protecting our plants, which are our genetic treasure, and transferring them to future generations.

Keywords

• Antioxidant
• Free Radical
• O. Alborosea
• Phenolic Substance
• Muş

O. Alborosea Bitkisinin Fenolik Bileşen Analizi ve Antioksidan Özellikleri

Abstract

Onosma bitkisi uzun uzun zamandan beri halk arasında tedavi edici olarak kullanılmaktadır. Çalışmamızda ülkemizde geleneksel tıpta kullanılan O. Alborosea Fisch. &C.A.Mey. ssp.'nın (O. alborosea) bitkisinin içerik analizi ve bazı biyolojik aktiviteleri araştırıldı. Bu amaçla, bitkinin metanol ve su ekstraktları alınarak, her bir ekstraktın toplam fenolik madde miktarı HPLC cihazı aracılığıyla belirlendi. Antioksidan aktivitelerini belirlemek için FRAP, CUPRAC indirgeme yöntemleri ile DPPH, ABTS radikali süpürme yöntemleri kullanıldı. Çalışmamızın sonuçlarına göre, su ve metanol ekstraktlarının standart antioksidanlara göre daha düşük aktivite gösterdikleri tespit edildi. Bunun sebebinin, içerdikleri fenolik miktarının oranıyla ilişkili olduğu söylenebilir. Mevcut bitki türüyle alakalı literatür taraması yapıldığında özellikle antioksidan çalışmalara rastlanmadığından çalışmamızın bu bitki türü ile yapılacak başka çalışmalara rehperlik edeceği, genetik hazinemiz olan bitkilerimizin korunması ve nesillere aktarılması açısından önem arz ettiği düşünülmektedir.

Keywords

• Antioksidan
• Serbest Radikal
• O. Alborosea
• Fenolik Madde
• Muş

Ethical Statement

Makale için etik kurul belgesi almaya ihtiyaç yoktur.

Thanks

Bitkinin tür tayinini yapan Muş Alparslan Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölüm Başkanı Prof. Dr. Murad Aydın ŞANDA'ya teşekkür ederiz.

References

1. [1] Turan N., Savci A., Buldurun K. Alan Y., Adigüzel R. Synthesis and Chemical Structure Elucidation of Two Schiff Base Ligands, Their Iron(II) and Zinc(II) Complexes, and Antiradical, Antimicrobial, Antioxidant Properties, Letters in Organic Chemistry, 13:5 343-351(9), 2016.
2. [2] Savcı A., Turan N., Buldurun K., Alkış M. E., Alan Y. Schiff base containing fluorouracil and its M(II) complexes: Synthesis, characterization, cytotoxic and antioxidant activities, Inorganic Chemistry Communications, 143 109780, 2022.
3. [3] Elkordy A. A., Haj-Ahmad R. R., Awaad A. S., Zaki R. M. An overview on natural product drug formulations from conventional medicines to nanomedicines: Past, present and future, Journal of Drug Delivery Science and Technology, 63 102459, 2021.
4. [4] Savcı A. Biological active compounds and biological activities of the foam used in the traditional kerebiç dessert, International Food Research Journal 29(5): 1067- 1077, 2022.
5. [5] Sarikurkcu C., Kirkan B., Ozer M. S., Ceylan O., Atilgan N., Cengiz M., Tepe B. Chemical characterization and biological activity of Onosma gigantea extracts, Industrial Crops and Products, 115 323-329, 2018.
6. [6] Hayta S., Polat R., Selvi S. Traditional uses of medicinal plants in Elazığ (Turkey), Journal of Ethnopharmacology, 154:3 613-623, 2014.
7. [7] Dalar A., Mukemre M., Unal M., Ozgokce F. Traditional medicinal plants of Ağrı Province, Turkey, Journal of Ethnopharmacology, 226 56-72, 2018.
8. [8] Noula E., Samanidou V. F., Assimopoulou A. N. et al. Solid-phase extraction for purification of alkannin/shikonin samples and isolation of monomeric and dimeric fractions, Analytical and Bioanalytical Chemistry 397 2221–2232, 2010.

9. [9] Zhang W. J., Su J., Tan M.Y., Liu G. L., Pang Y. J., Shen H. G., Qi J. L., Yang, Y. Expression analysis of shikonin-biosynthetic genes in response to M9 medium and light in Lithospermum erythrorhizon cell cultures Plant Cell, Tissue and Organ Culture, 101 (2) 135-142, 2010.
10. [10] Tekeli Y., Kılıç Ö., Altuner E. E. (2024). DNA Protective Assay and Some Biochemical Properties of Galium Species. Muş Alparslan Üniversitesi Fen Bilimleri Dergisi, 12(1), 24-30. https://doi.org/10.18586/msufbd.1451599.
11. [11] Kirkan B., Sarikurkcu C., Zengin G. Bioactive constituents, antioxidant effects and enzyme inhibitory properties of two Onosma species (Onosma trapezuntea and O. rigidum), South African Journal of Botany, 145 142-148, 2022.
12. [12] Menemen Y., Aytaç Z., Kandemir, A. Türkçe Bilimsel Bitki, Mantar, Suyosunu ve Bakteri Adları Yönergesi. Bağbahçe Bilim Dergisi, 8(3) 188-195, 2021.
13. [13] Savcı A., Koçpınar E. F., Alan Y., Kurşat M. Antioxidant, antimicrobial, and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. International Food Research Journal. 27:1 160-170, 2020.
14. [14] Oyaizu M. Studies on Products of Browning Reaction Prepared From Glucose Amin. The Japanese Journal of Nutrition and Dietetics, 44:6 307-315, 1986.
15. [15] Malhi R., Singh I., Carmieli R., Savci A., Sharma R. Copper(II) complexes of fused ring selenosemicarbazones: Synthesis, structure elucidation, biological activity and molecular modeling, Polyhedron, 233 116319, 2023.
16. [16] Blois M. S. Antioxidant Determinations by the Use of a Stable Free Radical, Nature, 181:4617 1199-1200, 1958.
17. [17] Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radical Biology and Medicine, 26:9-10 1231-1237, 1999.
18. [18] Ullah Wazir N., Amir Khan I., Javed A., Khan T., Jabbar A. Onosma hispidum L. extract reverses hyperlipidemia, hypertension, and associated vascular dysfunction in rats, Saudi Journal of Biological Sciences, 30(8) 103712, 2023.
19. [19] Saravanakumar K., Sarikurkcu C., Sarikurkcu R. T., Wang M. H., A comparative study on the phenolic composition, antioxidant and enzyme inhibition activities of two endemic Onosma species, Industrial Crops and Products, 142 111878, 2019.
20. [20] Baltaci N., Aydogdu N., Sarikurkcu C., Tepe B. Onosma gracilis (Trautv.) and O. oreodoxa (Boiss. & Heldr.): Phytochemistry, in silico docking, antioxidant and enzyme inhibitory activities, South African Journal of Botany, 143 410-417, 2021.
21. [21] Alhafez A., Savci A., Alan Y., Söylemez R., Kilic A. Preparation of Cu(II), Ni(II), Ti(IV), VO(IV), and Zn(II) metal complexes derived from novel vic-dioxime and investigation of their antioxidant and antibacterial activities, Chemistry&Biodiversity, 19:3 e202100768, 2022.
22. [22] Katanić Stanković J. S., Ceylan R., Zengin G., Matić S., Jurić T., Diuzheva A., Jeko J., Cziáky Z., Aktumsek A. Multiple biological activities of two Onosma species (O. sericea and O. stenoloba) and HPLC-MS/MS characterization of their phytochemical composition, Industrial Crops and Products, 144 112053, 2020.
23. [23] Sarikurkcu C., Sahinler S. S., Husunet M. T., Istifli E. S., Tepe B. Two endemic Onosma species (O. sieheana and O. stenoloba): A comparative study including docking data on biological activity and phenolic composition, Industrial Crops and Products, 154 112656, 2020.
24. [24] Ain Q. U., Singh I., Carmieli R., Savci A., Paul K., Sharma R. Substituted 2,5 thiophene dicarboxaldehyde bisthiosemicarbazones and their copper(II) complexes: Synthesis, structure elucidation, HSA binding, biological activities and docking studies, Journal of Molecular Structure, 1291 135996, 2023.
25. [25] Kilic A., Savci A., Alan Y., Beyazsakal L. The synthesis of novel boronate esters and N-Heterocyclic carbene (NHC)-stabilized boronate esters: Spectroscopy, antimicrobial and antioxidant studies, Journal of Organometallic Chemistry, 917 121268, 2020.
26. [26] Ozer M. S., Kirkan B., Sarikurkcu C., Cengiz M., Ceylan O., Atılgan N., Tepe B. Onosma heterophyllum: Phenolic composition, enzyme inhibitory and antioxidant activities, Industrial Crops and Products, 111 179-184, 2018.