An overview to the cytological, physiological, genetic and morphological effects of essential oils of Mentha longifolia

Year 2021, Volume: 9 Issue: 2, 891 - 897, 26.12.2021

Sedat Bozarı Güleray Ağar Derya Efe Ömer Bingöl

https://doi.org/10.18586/msufbd.1031259

Abstract

The present study was aimed to impress the cytological, physiological, genetic, and morphological effects of the essential oil obtained by hydro-distillation from Mentha longifolia (L.) HUDSON (L.) HUDSON against maize seeds. These naturally occurred compounds can be used instead of synthetic chemicals. To determine their biological activity, the oil was applied to Zea mays L. seeds at different doses. It was observed that the germination rate and mitotic division of the root tips were decreased with the increasing of the applied doses. Genomic stability was established by RAPD and the genetic differences between doses with AFLP technique. The alterations of RAPD and AFLP profiles did not support germination rate and mitotic index results while supporting each other. To determine the effects of the volatile on gene expression, the soluble protein level, and hormone levels were measured and the changes in the protein profile were determined by SDS-PAGE analyses. The independent changes were observed at hormones, soluble protein level, and protein profiles. It was concluded that the different content of oils showed a complicated effect on maize seeds. The maize seeds showed different reactions for protection.

Keywords

AFLP, RAPD, SDS-Page, Essential oil, Lamiaceae, Plant growth regulator

Project Number

BAP (2010/271)

Mentha longifolia uçucu yağlarının sitolojik, fizyolojik, genetik ve morfolojik etkilerine genel bir bakış

Abstract

Bu çalışma, Mentha longifolia (L.) HUDSON (L.) HUDSON'dan hidrodistilasyon ile elde edilen uçucu yağların mısır tohumlarına karşı sitolojik, fizyolojik, genetik ve morfolojik etkilerini belirlemeyi amaçlamıştır. Doğal olarak oluşan bu bileşikler, sentetik kimyasallar yerine kullanılabilir. Uçucu yağlar, biyolojik aktivitelerinin belirlenmesi için, Zea mays L. tohumlarına farklı dozlarda uygulandı. Uygulanan yağların dozlarının artmasıyla kök ucu uzunluğunun, çimlenme hızının ve mitotik bölünmenin azaldığı gözlemlendi. Yağların neden olduğu genomik stabilite RAPD, dozlar arasındaki genetik farklılıklar ise AFLP tekniği ile belirlendi. RAPD ve AFLP profillerindeki değişikliklerin benzer olduğu ancak çimlenme oranı ve mitotik indeks sonuçlarıyla uyuşmadığı görüldü. Uçucu bileşenlerin gen ekspresyonu üzerindeki etkilerini belirlemek için çimlendirilen tohumların çözünür protein seviyesi ve bitki büyüme düzenleyicileri seviyeleri ölçüldü. Protein profillerindeki değişiklikler SDS-PAGE ile belirlendi. Bitki büyüme düzenleyicileri seviyeleri, çözünür protein seviyesi ve protein profillerinde birbirinden bağımsız değişimler gözlendi. Uygulanan uçucu yağların mısır tohumları üzerine farklı parametrelerde birbirinden bağımsız etkiler gösterdiği belirlendi. Çimlenen tohumların, ekzojen maddelerden korunmak için farklı mekanizmalar geliştirdiği kanısına varıldı.

Keywords

Allelopati, Bitki büyüme düzenleyicileri, AFLP, RAPD, SDS-Page, Lamiaceae, Uçucu Yağlar

Supporting Institution

Atatürk Üniversitesi

Project Number

BAP (2010/271)

References

• Fletcher J., Bender C., Budowle B., Cobb W., Gold S., Ishimaru C., Luster D., Melcher U., Murch R., Scherm H. Plant pathogen forensics: capabilities, needs, and recommendations, Microbiol. Mol. Biol. Rev., 70:2 450-471, 2006.
• Sodaeizadeh H., Rafieiolhossaini M., Van Damme P. Herbicidal activity of a medicinal plant, Peganum harmala L., and decomposition dynamics of its phytotoxins in the soil, Indust. Crops Prod., 31:2 385-394, 2010.
• Bozari S., Agar G., Aksakal O., Erturk F.A., Yanmis D. Determination of chemical composition and genotoxic effects of essential oil obtained from Nepeta nuda on Zea mays seedlings, Toxicol. Industr. Health, 29:4 339-348, 2013.
• Gormez A., Bozari S., Yanmis D., Gulluce M., Agar G., Sahin F. Antibacterial activity and chemical composition of essential oil obtained from Nepeta nuda against phytopathogenic bacteria, J. Essent. Oil Res., 25:2 149-153, 2013.
• Cordell G.A. Biodiversity and drug discovery-a symbiotic relationship, Phytochemistry, 55:6 463-480, 2000.
• Kobayashi K., Itaya D., Mahatamnuchoke P., Pornprom T. Allelopathic potential of itchgrass (Rottboellia exaltata L.) powder incorporated into soil,Weed Biol. Manag., 8:1 64-68, 2008.
• Heywood V.H., Flowering plants of the world, BT Batsford Ltd, 1993.
• Castro-Vázquez L., Díaz-Maroto M., González-Viñas M., Pérez-Coello M. Differentiation of monofloral citrus, rosemary, eucalyptus, lavender, thyme and heather honeys based on volatile composition and sensory descriptive analysis, Food Chem., 112:4 1022-1030, 2009.
• Ben Farhat M., Jordán M.a.J., Chaouech-Hamada R., Landoulsi A., Sotomayor J.A. Variations in essential oil, phenolic compounds, and antioxidant activity of tunisian cultivated Salvia officinalis L., J,Agri. Food Chem., 57:21 10349-10356, 2009.
• Cheung S., Tai J. Anti-proliferative and antioxidant properties of Rosmarinus officinalis, Oncol. Rep., 17:6 1525-1532, 2007.
• Takaki I., Bersani-Amado L., Vendruscolo A., Sartoretto S., Diniz S., Bersani-Amado C., Cuman R. Anti-inflammatory and antinociceptive effects of Rosmarinus officinalis L. essential oil in experimental animal models, J.Medic. Food, 11:4 741-746, 2008.
• Bozari S. Determination of the Genotoxic Effects of the Potential Allelopathic Essential Ois Obtained from Various Species of Lamiaceae Family, PHD, Biology, Atatürk Üniversity, 2012.
• Oyedeji A., Afolayan A. Chemical composition and antibacterial activity of the essential oil isolated from South African Mentha longifolia L. subsp. capensis (Thunb.) Briq., The J. Essent.l Oil Res., 18: 57-59, 2006.
• Mimica-Dukic N., Popovic M., Jakovljevic V., Szabo A., Gašic O. Pharmacological studies of Mentha longifolia phenolic extracts. II. Hepatoprotective activity, Pharma. Biol., 37:3 221-224, 1999.
• Liu W., Li P., Qi X., Zhou Q., Zheng L., Sun T., Yang Y. DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis, Chemosphere, 61:2 158-167, 2005.
• Shultz R.W., Settlage S.B., Hanley-Bowdoin L., Thompson W.F. A trichloroacetic acid-acetone method greatly reduces infrared autofluorescence of protein extracts from plant tissue, Plant Mol. Biol. Rep., 23:4 405-409, 2005.
• Battal P., Tileklioğlu B. The effects of different mineral nutrients on the levels of cytokinins in maize (Zea mays L.), Turk. J.Bot., 25: 123-130, 2001.
• Cetin A., Bozari S. Study on the Synthesis, Antimicrobial Activity, and Rapd Analysis of 3-phenyl-1,5-bis(4-(trifluoromethyl)phenyl)-1H-pyrazole, J Biochem Tech., 11:3, 77-83.
• Morris J.W., Doumas P., Morris R.O., Zaerr J.B. Cytokinins in vegetative and reproductive buds of Pseudotsuga menziesii, Plant Physiol., 93:1 67-71, 1990.
• Türker M., Battal P., Aĝar G., Güllüce M., Şahin F., Erez M., Yildirim N. Allelopathic effects of plants extracts on physiological and cytological processes during maize seed germination, Allelopat. J., 21:2 273-286, 2008.
• Erez M.E. Lepidium draba L., Acroptilon repens (L.) DC., Thymus kotchyanus Boiss&Hohen. var. kotchyanus, Inula peacockiana (Aitch.&Hemsl.) Koravin, Salvia kronenburgei Rech. f. ve Phlomis armeniaca Wild. Bitkilerinin Allelopatik etkilerinin Araştrırılması, Doktora Tezi, Biology, Yüzüncü yıl Üniversitesi, 2009.
• Orhan F. Mentha longifolia L. Hudson Ssp. Longifolia’dan Elde Edilen Bazı Etken Maddelerin Ames/Salmonella ve Maya Delesyon Test Sistemleri ile Mutajen ve Antimutajen Özelliklerinin Belirlenmesi, phd, Biology, Atatürk Üniversity, 2010.
• Ahmad N., Fazal H., Ahmad I., Abbasi B.H. Free radical scavenging (DPPH) potential in nine Mentha species, Toxicol. Indust. Health, 28:1 83-89, 2012.
• Shah A.J., Bhulani N.N., Khan S.H., Gilani A.H. Calcium channel blocking activity of Mentha longifolia L. explains its medicinal use in diarrhoea and gut spasm, Phytother. Res., 24:9 1392-1397, 2010.
• Vokou D., Chalkos D., Karamanoli K., Microorganisms and allelopathy: a one-sided approach, Allelopathy, Springer, 2006.
• Tripathi A.K., Prajapati V., Ahmad A., Aggarwal K.K., Khanuja S.P. Piperitenone oxide as toxic, repellent, and reproduction retardant toward malarial vector Anopheles stephensi (Diptera: Anophelinae), J.Med. Entomol., 41:4 691-698, 2004.
• Thomas S.G., Rieu I., Steber C.M. Gibberellin metabolism and signaling. Vitamins and Hormones, 72: 289-338, 2005.
• Schempp H., Hippeli S., Elstner E.F., Hock B. Plant stress: avoidance, adaptation, defense, Plant Toxicol., 4th ed. New York: Marcel Dekker, 87-129, 2005.