Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris

Year 2022, Volume: 10 Issue: 2, 1013 - 1022, 26.12.2022

Belgin Erdem , Harun Çiftçi , Yunus Şahin

https://doi.org/10.18586/msufbd.1126542

Cited By: 1

Abstract

In this study, biosynthesis and in vitro phytochemical composition, antibacterial and antioxidant activities of silver nanoparticles were investigated by using aqueous leaf, flower and root extracts of Primula vulgaris (P. vulgaris subsp. vulgaris). The synthesized silver nanoparticles (AgNPs) were confirmed by color conversion and ultraviolet-visible (UV-visible) spectrophotometry. The appearance of a dark brown color and a UV absorption spectrum range at 440 nm confirmed the synthesized silver nanoparticles. The antimicrobial activity of silver nanoparticles synthesized from the leaf of P. vulgaris; S. aureus 25±1, S. epidermidis 20±1, P. aeruginosa 20±1, A. hydrophila 21±1, C. albicans 25±1, C. tropicalis 25±1, C. parapsilosis 22±1 and C. glabrata 20±1 mm zone diameter was determined. The most antimicrobial effect of P. vulgaris leaf aqueous extract; S. aureus 20±1, S. epidermidis 18±1, A.hydrophila 15±1, P. aeruginosa 12±2, C. albicans 18±1, C. glabrata 18±1, C.tropicalis15±2, and C. parapsilosis 15±2 mm zone diameter was revealed. The presence of flavonoids, terpenoids, protein, and carbohydrates was found to be higher in silver nanoparticles synthesized in the flower part of P. vulgaris, according to phytochemical screening. While saponins were detected in P. vulgaris root extracts, tannins and protein were detected in the leaf extract. The flower had the highest total phenolic extract content of the silver nanoparticle (29.08±0 mg GAE/g DW), while the leaf and root had the lowest total phenolic content of 9.06±0.5 and 8.64±3.3 mg GAE/g DW, respectively. The flower had the highest total phenolic extract content of the plant aqueous extracts (25.10±0.2 mg GAE/g DW), while the leaf and root had the lowest (8.28±0.5 and 5.20±0.0 mg GAE/g DW, respectively).The DPPH (1,1-Diphenyl-2-picrylhydrazil) assay was used to assess free radical scavenging activity. The antioxidant activity of AgNPs biosynthesized using P. vulgaris flower extract was 90.6 %, while P. vulgaris flower aqueous extracts were 86.3 %.
This can be concluded that silver nanoparticles synthesized using P. vulgaris flower extract are useful in the preparation of pharmacologically useful drugs.

Keywords

Primula vulgaris, Antimicrobial, Antioxidant, Silver nanoparticle

Supporting Institution

Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

Proje No: SAG.A4.19.001

Thanks

The authors express their gratitude to the research council of Kırşehir Ahi Evran University for the financial support during the course of this project.

Primula vulgaris'ten Sentezlenen Gümüş Nanopartiküllerin Antimikrobiyal ve Antioksidan Potansiyeli

Abstract

Bu çalışmada, Primula vulgaris'in (P. vulgaris subsp. vulgaris) sulu yaprak, çiçek ve kök ekstreleri kullanılarak gümüş nanopartiküllerin biyosentezi ve in vitro fitokimyasal bileşimi, antibakteriyel ve antioksidan aktiviteleri araştırılmıştır. Sentezlenen gümüş nanoparçacıklar (AgNP'ler), renk dönüşümü ve ultraviyole-görünür (UV-görünür) spektrofotometrisi ile doğrulandı. Koyu kahverengi rengin görünümü ve 440 nm'de bir UV absorpsiyon spektrum aralığı, sentezlenen gümüş nanoparçacıkları doğruladı. P. vulgaris’in yaprak ekstraktından sentezlenen gümüş nanopartiküldeki en fazla antimikrobiyal etki; S. aureus 25±1, S. epidermidis 20±1, P. aeruginosa 21±1, A. hydrophila 21±1, C. albicans 25±1, C. tropicalis 25±1, C. parapsilosis 22±1 ve C.glabrata 20±1 mm zon çapı ile belirlendi.P. vulgaris yaprak sulu ekstraktın en fazla antimikrobiyal etki; S. aureus 20±1, S. epidermidis 18±1, A.hydrophila 15±1, P. aeruginosa 12±2, C. albicans 18±1, C. glabrata 18±1, C.tropicalis15±2 ve C. parapsilosis 15±2 mm zon çapı ortaya konmuştur. Fitokimyasal taramaya göre P. vulgaris'in çiçek kısmında sentezlenen gümüş nanopartiküllerde flavonoidler, terpenoidler, protein ve karbonhidratların varlığı daha yüksek bulunmuştur. P. vulgaris kök ekstraktlarında saponinler tespit edilirken, yaprak ekstraktında tanen ve protein tespit edildi. P. vulgaris yaprak ekstraktından sentezlenen gümüş nanopartiküllerin antimikrobiyal aktivitesinin, P. vulgaris yaprak sulu ekstraktının antimikrobiyal aktivitesinden daha aktif olduğu bulundu. Çiçek, gümüş nanopartikülün en yüksek toplam fenolik ekstrakt içeriğine (29.08±0 mg GAE/g DW) sahipken, yaprak ve kök, sırasıyla 9.06±0.5 ve 8.64±3.3 mg GAE/g DW ile en düşük toplam fenolik içeriğe sahipti.
Bitki sulu ekstraktları arasında en yüksek toplam fenolik ekstrakt içeriği çiçekte bulunurken (25.10±0.2 mg GAE/g DW), yaprak ve kök en düşük (sırasıyla 8.28±0.5 ve 5.20±0.0 mg GAE/g DW) bulundu. DPPH (1,1-Difenil-2-pikrilhidrazil) tahlili, serbest radikal süpürme aktivitesini değerlendirmek için kullanıldı. P. vulgaris çiçek özütü kullanılarak biyosentezlenen AgNP'lerin antioksidan aktivitesi %90,6 iken, P.vulgaris çiçek sulu özütleri %86.3'tür.
Buradan P. vulgaris çiçek ekstresi kullanılarak sentezlenen gümüş nanopartiküllerin farmakolojik olarak faydalı ilaçların hazırlanmasında faydalı olduğu sonucuna varılabilir.

Keywords

Antimikrobiyal, Antioksidan, Gümüş nanopartikül, Primula vulgaris

Project Number

Proje No: SAG.A4.19.001

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