HPLC BASED CHARACTERIZATION OF BIOACTIVE COMPOUNDS OF Schizochytrium sp. MICROALGAE WITH ITS ANTIOXIDANT AND ENZYME INHIBITORY EFFECTS

Abstract

In this study, Schizochytrium sp. microalgae hexane, ethyl acetate, methanol and water extracts were investigated for bioactive compounds and tested for antioxidant and enzyme inhibitory effects. The presence of galangin (0.18 ppm for hexane extract-101.29 ppm for methanol extract) and 4-hexylresorcinol (20.70 ppm for hexane extract-29.70 ppm for methanol extract) was determined for the first time in Schizochytrium sp. microalgae extracts. The high antioxidant effective extract was methanol extract in DPPH• scavenging (18.46%), β-carotene linoleic acid (49.34%) and CUPRAC (A0.50: 158.82 µg/mL) assays, water extract in ABTS•+ scavenging (IC50: 309.13 µg/mL) and iron chelating assays. Schizochytrium sp. microalgae showed different levels of activity in AChE, BChE, tyrosinase, urease, α-amylase, and α-glucosidase inhibitory effects. Hexane extract (86.53%) exhibited superior urease inhibitory effect, with higher inhibition rate than thiourea (78.93%). In addition, methanol extract (66.17%) was observed as promising active in BChE inhibitory effect, hexane extract (60.28%) in α-amylase inhibitory effect, and ethyl acetate extract (34.64%) in α-glucosidase inhibitory effect. Taking together, these results reveal the potential utility of Schizochytrium sp. microalgae extracts for potential applications, particularly in the pharmaceutical industry.

Keywords

• Schizochytrium sp.
• Bioactive microalgae
• Antioxidant activity
• Enzyme inhibition activity
• Phenolic compounds

Schizochytrium sp. MİKROALGİNİN BİYOAKTİF BİLEŞİKLERİNİN HPLC İLE KARAKTERİZASYONU İLE ANTİOKSİDAN VE ENZİM İNHİBİSYON AKTİVİTELERİ

Abstract

Bu çalışmada, Schizochytrium sp. mikroalginin hekzan, etil asetat, metanol ve su ekstreleri biyoaktif bileşikleri açısından araştırılmıştır ve antioksidan ve enzim inhibisyon etkileri test edilmiştir. Galanginin (hekzan ekstresinde 0,18 ppm, metanol ekstresinde 101,29 ppm) ve 4-hekzilresorsinolün (hekzan ekstresinde 20,70 ppm, metanol ekstresinde 29,70 ppm) varlığı Schizochytrium sp. mikroalg ekstrelerinde ilk kez belirlenmiştir. En yüksek antioksidan etkili ekstre DPPH• radikal giderim (%18,46%), β-karoten linoleik asit (%49,34) ve CUPRAC (A0,50: 158,82 µg/mL) yöntemlerinde metanol ekstresi, ABTS•+ giderim (IC50: 309,13 µg/mL) ve demir şelatlama aktivitelerinde su ekstresi olmuştur. Schizochytrium sp., mikroalgi AChE, BChE, tirozinaz, üreaz, α-amilaz ve α-glukozidaz inhibisyon etkilerinde farklı seviyelerde aktivite göstermiştir. Hekzan ekstresi (%86,53) tiyoürenin (%78,93) inhibisyon değerinden daha yüksek inhibisyon değeriyle yüksek üreaz inhibisyon etkisi göstermiştir. Ayrıca, metanol ekstresi (%66,17) BChE inhibisyon etkisinde, hekzan ekstresi (%60,28) α-amilaz inhibisyon etkisinde ve etil asetat ekstresi (%34,64) α-glukozidaz inhibisyon etkisinde umut verici aktif olarak gözlenmiştir. Bu sonuçlar bir araya getirildiğinde, Schizochytrium sp. mikroalg ekstrelerinin özellikle ilaç endüstrisindeki potansiyel uygulamalar için faydaları ortaya çıkmaktadır.

Keywords

• Schizochytrium sp.
• Bioaktif mikroalg
• Antioksidan aktivite
• Enzim inhibisyon aktivitesi
• Fenolik bileşikler

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