VIRUS ERADICATION FROM PLANTS VIA NOVEL BIOTECHNOLOGICAL PROCESSES: ONE STEP FREEZING METHODS BASED ON VITRIFICATION OF CRYOTHERAPY TECHNIQUES

Abstract

Plant diseases are caused by microscopic pathogens including viruses, viroids, phytoplasmas, bacteria, and fungi. Pathogenic microorganisms are difficult to diagnose and treat as a consequence of their small size and thus rely on technologically advanced methodologies for recognition of chemicals, proteins or molecular fragments specific to different organisms. Cryotherapy is an coming out method stemming from the use of cryopreservation, a cold treatment used for the long-term storage and preservation of all plant materials. It has recently been accepted that cryotherapy is an effective method for elimination viruses from plant shoot tips. This review covers different one step-freezing methods based on vitrification of cryotheraphy for virus eliminations from plants. In addition, the advantages and disadvantages as well as future prospects of each methods have also been discussed.

Keywords

• Cryotherapy, ELISA, Plant viruses, RT-PCR, Virus Eradication, Vitrification

YENİ BİYOTEKNOLOJİK YÖNTEMLERLE BİTKİLERDEN VİRÜS ELİMİNASYONU: KRİYOTERAPİ TEKNİKLERİNİN VİTRİFİKASYON TEMELLİ TEK AŞAMALI DONDURMA YÖNTEMLERİ

Abstract

Bitki hastalıkları, virüs, viroid, fitoplazma, bakteri ve mantarları kapsayan mikroskobik patojenler tarafından oluşturulur. Oldukça küçük organizmalar oldukları için, patojenik mikroorganizmaların tanısı ve tedavisi zordur ve bu nedenle, farklı organizmalara özgü kimyasalların, proteinlerin veya moleküler fragmentlerin tanısı için ileri teknolojili yöntemler gerekir. Kriyoterapi, tüm bitki materyallarinin korunması ve uzun süreli saklanması için kullanılan, bir soğuk uygulamalı yöntem olan kriyoprezervasyonun kullanımını temel alan bir metot olarak geliştirilmiştir. Yakın zamanda kabul gören kriyoterapi, bitki sürgün uçlarından virüslerin eliminasyonu için etkin bir yöntemdir. Bu derleme çalışması, bitkilerden virüs eliminasyonu için, kriyoterapinin vitrifikasyon temelli farklı tek aşamalı dondurma tekniklerini kapsamaktadır. Ek olarak, her bir tekniğin gelecekteki uygulamalarda, avantaj ve dez avantajları da tartışılmıştır.

Keywords

• Bitki virüsleri, ELISA, Kriyoterapi, RT-PCR, Virüs Eliminasyonu, Vitrifikasyon

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