Meme Kanserinde İnflamasyon Tümör Mikroçevresi ve Mast Hücreleri

Year 2025, Volume: 12 Issue: 2, 155 - 160, 28.08.2025

Leyla Tekin , Melek Ünçel

https://doi.org/10.47572/muskutd.1660645

Abstract

Tümör mikroçevresi fibroblastlar, endotel ve bağışıklık hücreleri ve hücre dışı matristen oluşur ve kanseri çevreler. Mast hücreleri, anjiyogenez, stroma immünomodülasyonu ve kanserlerde doku yeniden şekillenmesi ile ilişkilidir. Farklı tümörlerde, tümör mikroçevresine göre antitümör veya güçlendirici etkilere sahip olabilir. Meme kanseri stromasındaki bağışıklık hücreleri, kanser davranışını şekillendiren inflamatuar mikroçevreyi oluşturur. 2017-2020 yılları arasında meme kanseri nedeniyle mastektomi geçiren 85 kadın hastadan alınan örnekler retrospektif olarak değerlendirildi. Hematoksilen-eozin boyalı slaytlar temelinde immünohistokimyasal boyama yapıldı. Mast hücre yoğunluğu, üç yüksek yoğunluklu alanda sayılarak hotspot yöntemi kullanılarak değerlendirildi. Hem tümör çevresi hem de tümör içi yoğunluklar ayrı ayrı kaydedildi ve ortalama sayımlar üç alandan hesaplandı. CD117 üç büyük büyütmede (400x) sayıldı ve tümör içi ve tümör çevresi hücreler ayırt edildi. Tümör içi fibrozis ve lenfositlerin varlığı puanlandı. Sonuçta; tümör çevresi mast hücre sayısı ER negatif olgular ve metastatik lenf nodu sayısı yüksek olgularda ortalamanın altında bulundu. Lobüler karsinom ile tümör içi mast hücre yoğunluğu arasında ters bir ilişki gözlendi. Ek olarak, tümör içi mast hücre yoğunluğu ile artmış tümör boyutu arasında anlamlı bir ters ilişki vardı. Ortalamanın altında tümör içi mast hücre sayısına sahip vakalar, ER negatifliği, yüksek Ki67 proliferasyon indeksi, luminal grup vakaları ve fibrozis yokluğu ile anlamlı bir şekilde ilişkiliydi. Tümörlerde düşük mast hücre sayısı, zayıf prognostik parametrelerle ilişkili bulundu.

Keywords

Mast Hücreleri , CD117 , Meme Karsinomu

Supporting Institution

yok

Inflammation Tumor Microenvironment and Mast Cells in Breast Cancer

Abstract

The tumor microenvironment consists of fibroblasts, endothelial and immune cells, and extracellular matrix and envelops the cancer. Mast cells are associated with angiogenesis, immunomodulation of the stroma, and tissue remodeling in cancers. In different tumors, it may have antitumor or potentiating effects in relation to the tumor microenvironment. Immune cells in breast cancer stroma form the inflammatory microenvironment that shapes cancer behavior. Samples taken from 85 female patients who underwent mastectomy due to breast cancer between 2017 and 2020 were evaluated retrospectively. Immunohistochemical staining was performed on the basis of hematoxylin-eosin stained slides. Mast cell density was assessed using the hot-spot method, counting in three high-density areas. Both peritumoral and intratumoral densities were recorded separately, and average counts were calculated from the three fields. CD117 was counted at three high magnifications (400x), and intratumoral and peritumoral cells were distinguished. The presence of intratumoral fibrosis and lymphocytes was scored . Peritumoral mast cell count was found to be below average in ER-negative cases and in cases with high metastatic lymph node count. An inverse relationship was observed between lobular carcinoma and intratumoral mast cell density. In addition, there was a significant inverse relationship between intratumoral mast cell density and increased tumor size. Cases with below average intratumoral mast cell count were significantly associated with ER negativity, high Ki67 proliferation index, , luminal group cases and absence of fibrosis. Low mast cell count in tumors was found to be associated with poor prognostic parameters.

Keywords

Mast Cells , CD117 , Breast Carcinoma

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