Rize’den Toplanan Altı Bitkinin İn Vitro Üreaz İnhibe Edici ve Antioksidan Aktivitesi

Abstract

Giriş ve amaç: Üreaz enzimi, Helicobacter pylori nedenli peptik ülserin tedavisi için yeni ilaç adayı bileşiklerin hedeflerinden biri haline gelmiştir. Bu çalışmada Rize’den toplanan altı yabani bitkinin [Daphne pontica L., Lotus corniculatus L., Lythrum salicaria L., Medicago sativa L., Potentilla reptans L. ve Senecio leucanthemifolius subsp. vernalis (Waldst. & Kit.) Greuter] üreaz enzimini inhibe edici aktivitesinin belirlenmesi amaçlanmıştır. Bununla birlikte peptik ülserin önlenmesinde rol oynayan mekanizmalardan biri olan antioksidan aktiviteleri belirlenmiş, toplam fenol ve flavonoit içerikleri de tespit edilmiştir.
Yöntem: Bitkiler Rize Merkez ve İkizdere ilçesinden toplanıp %80’lik etanol ekstreleri hazırlanmış ve üreaz enzimini inhibe edici aktiviteleri belirlenmiştir. Hazırlanan ekstrelerin antioksidan aktivitesi DPPH, ABTS ve CUPRAC testleri ile tespit edilmiş, toplam fenol ve flavonoit miktar tayinleri yapılmıştır.
Bulgular: İn vitro testler sonucunda S. leucanthemifolius subsp. vernalis’in %80 etanol ekstresi üreaz enzimini düşük oranda inhibe ederken (%13,7) diğer çalışılan ekstrelerin daha kuvvetli (% 24,6 ve %31,3 arasında değişen oranlarda) inhibe ettiği görülmüştür. Çalışılan ekstrelerin genel olarak kuvvetli antioksidan aktiviteye sahip olduğu belirlenmiştir. En yüksek toplam fenol ve flavonoit içerikleri sırasıyla L. salicaria ve L. corniculatus’da tespit edilmiştir.
Sonuç: Çalışmaya dâhil edilen bitkilerin genel olarak üreaz enzimini belirli bir düzeyde inhibe ettiği belirlenmiştir. D. pontica, L. salicaria ve P. reptans yüksek antioksidan aktivite, toplam fenol ve flavonoit içerikleri ile öne çıkmıştır.

Keywords

• Üreaz
• Daphne pontica
• Lotus corniculatus
• Lythrum salicaria
• Medicago sativa
• Potentilla reptans
• Urease

In Vitro Urease Inhibitory and Antioxidant Activity of Six Plants Collected from Rize

Abstract

Introduction: The urease enzyme has become one of the targets of new drug candidate compounds for the treatment of peptic ulcer caused by Helicobacter pylori. In this study, it was aimed to determine the urease enzyme inhibitory activity of six wildly grown plants [Daphne pontica L., Lotus corniculatus L., Lythrum salicaria L., Medicago sativa L., Potentilla reptans L. and Senecio leucanthemifolius subsp. vernalis (Waldst. & Kit.) Greuter] collected from Rize. In addition, their antioxidant activities, one of the mechanisms that play a role in the prevention of peptic ulcer, and total phenol and flavonoid contents were also determined.
Methods: Plants were collected in Rize Center and İkizdere district, 80% ethanol extracts were prepared from their aerial parts and their urease enzyme inhibitory activity was determined. The antioxidant activities of the prepared extracts were determined with DPPH, ABTS and CUPRAC tests, and their total phenol and flavonoid amounts were determined.
Results: As a result of in vitro tests, 80% ethanol extract of S. leucantemifolius subsp. vernalis was seen to inhibit urease enzyme at a low rate (13,7%); while other studied extracts moderately inhibited it more strongly (at rates varying between 24,6% and 31,3%). It was determined that studied extracts generally have strong antioxidant activity. The highest total phenol and flavonoid contents were detected in L. salicaria and L. corniculatus, respectively.
Conclusion: It was determined that the plants included in the study generally inhibited the urease enzyme at a certain level. D. pontica, L. salicaria and P. reptans stood out with their high antioxidant activity, total phenol and flavonoid contents. Although very strong urease inhibitory activity was not observed with extracts; compounds, that could be isolated by bioactivity guided fractionation studies or their derivatives, could possibly show strong urease inhibitory activity and new urease inhibitory compounds could be found.

Keywords

• Urease
• Daphne pontica
• Lotus corniculatus
• Lythrum salicaria
• Medicago sativa
• Potentilla reptans

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