Comparison of Some Zingiberaceae Drugs in Terms of Antioxidant Activities

Abstract

Objective: In this study, it was aimed to comparatively investigate the antioxidant activity determinations and the total phenolic substance and total flavonoid amount determinations of methanol and water extracts prepared from the drugs Curcuma longa L., Zingiber officinale Roscoe, Alpinia officinarum Hance and Elettaria cardamomum (L.) Maton belonging to the Zingiberaceae family. Method: Our study was carried out between 12.01.2022-01.04.2022. The drugs were supplied from a company on 12.01.2022 and their aqueous and methanolic extracts were prepared, total phenolic substance amounts were determined by the Folin-Ciocalteu method, flavonoid amounts were determined by the aluminum chloride method and antioxidant activity was determined by the DPPH method. Results: Turmeric-MeOH extract showed the highest antioxidant activity (6,04 µg/ml), cardamom-water extract showed the lowest antioxidant activity (53,24 µg/ml). The highest amount of total phenolic substance was found in galangal methanol extract (400,53 µg gallic acid/mg) and turmeric methanol extract (301,72 µg gallic acid/mg). The highest amount of flavonoids was determined in turmeric-MeOH (615,51 µg quercetin/mg) and galangal-MeOH (400,53 µg gallic acid/mg) extracts. Conclusion: As a result of our study, it was seen that turmeric and galangal methanol extracts had the highest phenolic substance content and accordingly showed the highest antioxidant activity. The richest extract in terms of flavonoids was turmeric methanol extract. The results obtained in the antioxidant activity experiment showed positive correlation with the total phenolic and flavonoid contents of the extracts. Key words:

Keywords

• Antioxidant activity
• Flavonoid
• Zingiberaceae

Bazı Zingiberaceae Droglarının Antioksidan Aktivite Açısından Karşılaştırılması

Abstract

Amaç: Bu çalışmada Zingiberaceae familyasına ait olan Curcuma longa L., Zingiber officinale Roscoe, Alpinia officinarum Hance ve Elettaria cardamomum (L.) Maton droglarından hazırlanan metanol ve su ekstrelerinin antioksidan aktivite tayinleri ile toplam fenolik madde ve toplam flavonoit miktar tayinlerini karşılaştırmalı olarak araştırmak hedeflenmiştir. Yöntem: Çalışmamız 12.01.2022-01.04.2022 tarihleri arasında gerçekleştirilmiştir. Droglar, bir firmadan 12.01.2022 tarihinde temin edilerek sulu ve metanollü ekstreleri hazırlanmış, toplam fenolik madde miktarları Folin-Ciocalteu yöntemi ile, flavonoit miktarları alüminyum klorür yöntemi ile ve antioksidan aktivite tayinleri ise DPPH (2,2-diphenyl-1-pcyrylhydrazyl) yöntemiyle yapılmıştır. Bulgular: En yüksek antioksidan aktiviteyi zerdeçal-MeOH ekstresi (6,04 µg/ml), en düşük antioksidan aktiviteyi ise kakule-su ekstresi (53,24 µg/ml) göstermiştir. Toplam fenolik madde miktarı, en yüksek havlıcan metanol ekstresinde (400,53 µg gallik asit/mg) ve zerdeçal metanol ekstresinde (301,72 µg gallik asit/mg) bulunmuştur. En yüksek flavonoit miktarı ise zerdeçal-MeOH (615,51 µg kersetin/mg) ve havlıcan-MeOH (400,53 µg gallik asit/mg) ekstrelerinde saptanmıştır. Sonuç: Çalışmamız sonucunda zerdeçal ve havlıcan metanol ekstrelerinin en yüksek fenolik madde içeriğine sahip olduğu görülmüş ve buna bağlı olarak da en yüksek antioksidan aktiviteyi göstermişlerdir. Flavonoit bakımından en zengin ekstre zerdeçal metanol ekstresi olmuştur. Antioksidan aktivite deneyinde elde edilen sonuçlar, ekstrelerin toplam fenolik ve flavonoid içerikleriyle pozitif korelasyon göstermiştir. Anahtar kelimeler:

Keywords

• Antioksidan aktivite
• Flavonoit
• Zingiberaceae

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