Research Article

The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students

Volume: 16 Number: 1 January 23, 2026
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The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students

Abstract

Objective: Medical students struggle with exam anxiety, which negatively affects cognitive and academic performance. Aromatherapy has become popular as an alternative to pharmacological treatments. This study examines how juniper and thyme essential oils psychophysiologically affect undergraduate students' exam anxiety. Juniper and thyme aroma inhalation was tested on asprosin, cyclic guanosine monophosphate response element binding protein (CREB), cortisol, and exam anxiety in students taking exams. Method: A random sample of 80 healthy students aged 18-25 from the Faculty of Medicine was collected during final exams. Participants were their own controls, and saliva was collected before and after aromatherapy. Saliva samples were tested for asprosin, CREB, and cortisol using ELISA. Heart rate, oxygen saturation, systolic pressure, diastolic pressure, and body temperature were monitored before and after aroma inhalation. Findings: According to the findings of this study, juniper and thyme aromatherapy were effective in increasing the average saliva levels of Asprosin, CREB, and cortisol hormones, with the most significant decrease observed in the groups receiving juniper aromatherapy (p<0.001). Juniper aromatherapy affected Systolic Blood Pressure (SBP) and Diastolic Blood Pressure (DBP) (p<0.05) but did not affect Heart Rate (HR), Peripheral Capillary Oxygen Saturation (SPO2), or temperature (p>0.05). Thyme aromatherapy did not affect SBP, DBP, HR, SPO2, or temperature. Conclusion: The limbic system, which controls memory and emotions, is linked to smell. Our study has provided original evidence that inhaling scents affects Asprosin, CREB, cortisol, and other physiological indicators in university students during exams, and offers supportive findings for alleviating exam anxiety.

Keywords

Ethical Statement

Ethics committee approval (08/07/2024/Decision No. 98) and institutional approval were obtained from Ankara Medipol University non-invasive ethical committee in Turkey. This study was conducted after re-ceiving ethical approval from Ankara Medipol University non-invasive ethical committee of Medical Sci-ences with the ethical code E-81477236-604.01.01-3329, dated July 07, 2024. The participants were in-formed about the study in accordance with the Declaration of Helsinki, and their consent was obtained via the informed consent form. Participants are assured of the opportunity to withdraw from the study at any time, as well as the confidentiality of their personal information. All approaches were executed in compliance with pertinent rules and legislation.

References

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Details

Primary Language

English

Subjects

Traditional, Complementary and Integrative Medicine (Other)

Journal Section

Research Article

Publication Date

January 23, 2026

Submission Date

October 5, 2025

Acceptance Date

January 7, 2026

Published in Issue

Year 2026 Volume: 16 Number: 1

APA
Böyük Özcan, G., Bayraktar, B., Bozok, Ü. G., & Orkun Erkiliç, T. (2026). The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students. Lokman Hekim Dergisi, 16(1), 256-267. https://doi.org/10.31020/mutftd.1797534
AMA
1.Böyük Özcan G, Bayraktar B, Bozok ÜG, Orkun Erkiliç T. The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students. Lokman Hekim Dergisi. 2026;16(1):256-267. doi:10.31020/mutftd.1797534
Chicago
Böyük Özcan, Gülbahar, Bülent Bayraktar, Ümmü Gülşen Bozok, and Tuğçe Orkun Erkiliç. 2026. “The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students”. Lokman Hekim Dergisi 16 (1): 256-67. https://doi.org/10.31020/mutftd.1797534.
EndNote
Böyük Özcan G, Bayraktar B, Bozok ÜG, Orkun Erkiliç T (January 1, 2026) The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students. Lokman Hekim Dergisi 16 1 256–267.
IEEE
[1]G. Böyük Özcan, B. Bayraktar, Ü. G. Bozok, and T. Orkun Erkiliç, “The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students”, Lokman Hekim Dergisi, vol. 16, no. 1, pp. 256–267, Jan. 2026, doi: 10.31020/mutftd.1797534.
ISNAD
Böyük Özcan, Gülbahar - Bayraktar, Bülent - Bozok, Ümmü Gülşen - Orkun Erkiliç, Tuğçe. “The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students”. Lokman Hekim Dergisi 16/1 (January 1, 2026): 256-267. https://doi.org/10.31020/mutftd.1797534.
JAMA
1.Böyük Özcan G, Bayraktar B, Bozok ÜG, Orkun Erkiliç T. The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students. Lokman Hekim Dergisi. 2026;16:256–267.
MLA
Böyük Özcan, Gülbahar, et al. “The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students”. Lokman Hekim Dergisi, vol. 16, no. 1, Jan. 2026, pp. 256-67, doi:10.31020/mutftd.1797534.
Vancouver
1.Gülbahar Böyük Özcan, Bülent Bayraktar, Ümmü Gülşen Bozok, Tuğçe Orkun Erkiliç. The Effectiveness of Juniper and Thyme Essential Oils in Reducing the Physiological Symptoms of Test Anxiety in Medical Students. Lokman Hekim Dergisi. 2026 Jan. 1;16(1):256-67. doi:10.31020/mutftd.1797534

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                                                                  This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

  

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