Yenilebilir mantar Macrolepiota procera kullanılarak yeşil sentez yöntemiyle sentezlenen gümüş nanopartiküllerinin in-vitro biyolojik aktivitelerinin araştırılması

Year 2022, Volume: 12 Issue: 1, 198 - 208, 24.01.2022

Serpil Gonca

https://doi.org/10.31020/mutftd.1031151

Abstract

Amaç: Mantarlar, çok çeşitli sekonder metabolitler üretebilmeleri nedeniyle halk arasında geleneksel tedavide kullanıldıkları bilinmektedir. Bu çalışmada, daha önce yapılan çalışmalarda anti-kanser, antioksidan, antimikrobiyal gibi çeşitli biyolojik aktiviteleri olduğu bildirilen gümüş nanopartiküllerin (AgNP'ler) Macrolepiota procera aracılı sentezlenmesi ve bu AgNP'lerin çeşitli in-vitro biyolojik aktivitelerinin araştırılması amaçlanmıştır.
Yöntem: Sentezlenen Mp-AgNP'ler, transmisyon elektron mikroskobu (TEM) kullanılarak karakterize edildi. Sentezlenen AgNP'lerin antimikrobiyal, biyofilm inhibisyonu ve hücre canlılığı inhibisyonu, DNA bölünmesi, DPPH aktivitesi gibi çeşitli biyolojik aktiviteleri in-vitro olarak incelenmiştir.
Bulgular: Mp-AgNP'lerin en yüksek DPPH süpürme aktivitesi 200 mg/L konsantrasyonda %92.72 olarak tespit edildi. Mp-AgNP'ler, E. coli pBR322 plazmid DNA'sında tek zincir kırığına neden oldu. Mp-AgNP'ler, test edilen mikroorganizmalara karşı orta düzeyde antimikrobiyal aktivite gösterdi. Ayrıca, Mp-AgNP'lerin P. aeruginosa ve S. aureus'a karşı biyofilm inhibisyon aktivitesi, 200 mg/L'de sırasıyla %65.80 ve %72.60 idi. Mp-AgNP'lerin E. coli hücre canlılığının inhibisyon aktivitesi, 500 mg/L'de %99,99 olarak tespit edildi.
Sonuç: Çalışmada elde edilen bulgulara göre, M. procera'nın AgNP sentezinde kullanılması, düşük maliyetli, çevre dostu, yüksek verime sahip olması ve insan sağlığına toksik olmaması nedeniyle özel önem taşımaktadır. Ayrıca yeni sentezlenen AgNP'ler yapılacak ileri çalışmalardan sonra farklı uygulamalar için etkin bir şekilde kullanılabilirler.

Keywords

Nanopartikül, antioksidan, antimikrobiyal aktivite, mikrobiyal hücre canlılığı, biyofilm inhibisyonu

Investigation of in-vitro biological activities of silver nanoparticles synthesized by green synthesis method using wild edible mushroom Macrolepiota procera

Abstract

Aim: Mushrooms known that to be used in traditional treatment among the people as they can generate a large diversity of secondary metabolites. In the present study, it was aimed to synthesized silver nanoparticles (AgNPs) mediated Macrolepiota procera, which is known to have diverse biological activities such as anticancer, antioxidant, antimicrobial in previous studies, and to investigated various in-vitro biological activities of these AgNPs.
Materials and Methods: Synthesized Mp-AgNPs were characterized using transmission electron microscopy (TEM). Various biological activities including antimicrobial, biofilm inhibition and cell viability inhibition, DNA cleavage, DPPH activity of synthesized AgNPs were investigated in-vitro.
Results: The highest DPPH scavenging activity of Mp-AgNPs was found as 92.72%, at 200 mg/L concentration. Mp-AgNPs caused single strain break in the E. coli pBR322 plasmid DNA. Mp-AgNPs showed moderate antimicrobial activity against tested microorganisms. Furthermore, the biofilm inhibition activity of Mp-AgNPs toward P. aeruginosa and S. aureus was 65.80% and 72.60% at 200 mg/L, respectively. Inhibition activity of E. coli cell viability of Mp-AgNPs was found as 99.99% at 500 mg/L.
Conclusion: From the findings obtained in the study , the use of M. procera in the AgNPs synthesis it is important private regard due to its low cost, eco-friendly, high yield and non-toxicity human health. In addition, newly synthesized AgNPs can be used effectively for different applications after further studies.

Keywords

Nanoparticles, antioxidant, antimicrobial activity, microbial cell viability, biofilm inhibition

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