Utilization of Polyimide Materials for Enhanced Electrochemical Detection of Cholesterol

Year 2024, Volume: 5 Issue: 1, 41 - 46, 29.06.2024

Fatma Bilge Emre

https://doi.org/10.46572/naturengs.1488623

Abstract

The objective of this study was to utilise a range of polyimide materials for the determination of cholesterol. Cholesterol is a waxy, fat-like substance produced by the liver and is essential for the construction of cells and the production of vitamins and other hormones. Blood cholesterol levels are an important parameter in the diagnosis and treatment of a number of clinical diseases, including arteriosclerosis, cardiovascular ailments, hypertension and others. Cholesterol levels in the blood were measured using a number of chemical and enzymatic methods. Electrochemical tests were conducted using Cyclic Voltammetry (CV) and Time Base (TB) techniques. The surface morphologies of the modified electrode surfaces were examined in detail using scanning electron microscopy (SEM) techniques. In the enzymatic reaction, hydrogen peroxide was formed as a result of the enzymatic reaction between cholesterol and cholesterol oxidase, which was measured at 0.650–0.750 V against Ag/AgCl. In this study, poly[1,3,5-tri(aminophenyl)hegzahydro-1,3,5-pyromellithimide] and poly[2,4,6-triaminopirimidyn benzophenondiimide] were employed as polymeric materials to immobilise cholesterol oxidase. It was hypothesised that these two polyimides could be utilised as an appropriate medium for the immobilisation of cholesterol oxidase.

Keywords

polyimide, cholesterol oxidase, polymer matrix, biosensor.

References

• Lang, J.K., (1990). Quantitative determination of cholesterol in liposome drug products and raw materials by high-performance liquid chromatography. Journal of Chromatography A, 16,157-163.
• Trettnak, W., Lionti, I., Macsini, M.M., (1993). Cholesterol biosensors prepared by electropolymerization of pyrrole, Electroanalysis, 5, 753-763.
• Charpentier, L., Murr, N. El., (1995). Amperometric determination of cholesterol in serum with use of a renewable surface peroxidase electrode, Analytical Chimica Acta, 318, 89-93.
• Tan, X., Li, M., Cai, P., Luo, L., Zou, X., (2005). An amperometric cholesterol biosensor based on multiwalled carbon nanotubes and organically modified sol-gel / chitosan hybrid composite film, Analytical Biochemistry, 337, 111-120.
• Pioch, D., Lozano, P., Frater C. and Graille, J., (1992). A quick method for cholesterol titration in complex media, Fat Science Technology 94, 268-272.
• Guilbault, G. G., Kauffmann, J., (1987). Enzyme-based electrodes as analytical tools, Biotechnology Apply Biochemistry, 9, 95-113.
• Bokoch, M. P., Devadoss, A., Palencsár, M. S., Burgess, J. D., (2004). Steady-state oxidation of cholesterol catalyzed by cholesterol oxidase in lipid bilayer membranes on platinum electrodes, Analytical Chimica Acta, 519, 47-55.
• Brahim, S., Narinesingh, D., Guiseppi-Elie, A., (200)1. Amperometric determination of cholesterol in serum using a biosensor of cholesterol oxidase contained within a poypyrole-hydrogel membrane, Analytica Chimica Acta, 448, 27-36.
• Kumar, A., Rajesh, A., Chaubey, Grover, S.K., Malhotra, B.D., (2001). Immobilization of cholesterol oxidase and potassium ferricyanide on dodecylbenzene sulfonate ıon-doped polypyrrole film, Journal of Applied Polymer Science, 82, 3486-3491.
• Bongiovanni, C., Ferri, T., Poscia, A., Varalli, M., Santucci, R., Desideri, A., (2001). An electrochemical multienzymatic biosensor for detemination of cholesterol, Bioelectrochemistry, 54, 17-22.
• Ekinci, E., Karagözler, A.A., Karagözler, A.E., (1996). Electrochemical Synthesis and Sensor Application of Poly(1,4-diaminobenzene), Synthetic Metals,79, 57-61.
• Ekinci, E., Öğünç, S.T., Karagözler, A.E., (1998). Electrochemical poly(1,3-phenylenediamine) synthesis as enzyme immobilization media, Journal of Applied Polymer Science, 68, 145-152.
• Güngör, Ö., Özcan, İ., Ali Erdoğan, M., Ateş, B., & Köytepe, S. (2019). Differential Pulse Voltammetric (DPV) Determination of the Local Anesthetic Bupivacaine using Polyimide Membrane-Based Electrodes. Analytical Letters, 53(2), 228–244.
• Aksoy, B., Güngör, Ö., Köytepe, S., & Seçkin, T. (2016). Preparation of Novel Sensors Based on Polyimide Membrane for Sensitive and Selective Determination of Dopamine. Polymer-Plastics Technology and Engineering, 55(2), 119–128.
• Güngör, Ö., Paşahan, A., Aksoy, B., Köytepe, S., Vd. (2020). Voltammetric Analysis Of Melatonin And Dopamine By Using Graphene Oxide/Polyimide Based Biosensor. İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 8(2), 367-382.
• Ekinci, E., (1999). Preparation and sensor properties of poly (o-toluidine) film, Polymer Bullettin, 42, 693-699.
• Ekinci, E., Emre, F.B., Köytepe, S., and Seçkin, T., (2005). Preparation, characterization and H2O2 electivity of hyperbranched polyimides, containing triazine. Journal of Polymer Research, 12 205-210.
• Köytepe, S., Paşahan, A., Ekinci, E., Seçkin, T. 2005. Synthesis, characterization and H2O2 - sensing properties of pyrimidine-based hyperbranched polyimides European Polymer Journal, 41, 121-127.
• Vasudevan, P. T., Zhou, T., (1997). Enzymatic assay of cholesterol by reakrion rate measurements, Biotechnology and Bioengineering, 53, 391-396.
• Li, J., Peng, T., Peng, Y., (2003). A cholesterol biosensor based on entrapment of cholesterol oxidase in a silisic sol-gel matrix at a prussian blue modified electrode, Electroanalysis, 15, 1031-1037.
• Li, G., Liao, J. M., Hu, G. Q., Ma, N. Z, Wu, P.J., (2005). Study of carbon nanotube modified biosensor for monitoring total cholesterol in blood, Biosensors and Bioelectronics, 10, 2140-2144.
• Srisawasdi, P., Jearanaikoon, P., Wetprasit, N., Sriwanthana, B., Kroll, M. H., Lolekha, P. H., (2005). Performance characteristics of cholesterol oxidase for kinetic determination of total cholesterol, Journal of Clinical Laboratory Analysis,19, 247-252.
• Arya, S. K., Solanki, P. R., Singh, R. P., Pandey, M. K., Data, M., Malhotra, B. D., (2006). Application of octadecanethiol self-assembled monolayer to cholesterol biosensor based on surface plasmon rsonance technique, Talanta, 69, 918-925.
• López, M. S-P., López-Cabarcos, E., López-Ruiz, B., (2006). Organic phase enzyme electrodes, Biomolecular engineering, 23, 135-147.
• Emre, F.B., (2007), Kolestrol Biyosensörü Tasarımında Bazı Polimerik Materyallerin Enzim İmmobilizasyon Ortamı Olark Kullanımı, Doktora Tezi, İnönü Üniversitesi Fen Bilimleri Enstitüsü, Malatya.
• Doukyu N, Aono R. (1998).Purification of Extracellular Cholesterol Oxidase with High Activity in the Presence of Organic Solvents from Pseudomonas sp. Strain ST-200. Appl Environ Microbiol, 64, 1929-1932.