Glucose-Sensitive Biosensor Design by Zinc Ferrite (ZnFe2O4) Nanoparticle-Modified Poly (o-toluidine) Film

Year 2023, Volume: 8 Issue: 3, 202 - 213, 15.12.2023

Ali Tuncay Ozyilmaz , Esiye Irem Bayram

https://doi.org/10.28978/nesciences.1405173

Cited By: 2

Abstract

Glucose oxidase (GOD) immobilized poly(o-toluidine) (POT) coated Pt electrode was designed for glucose-sensitive biosensor. Since POT film structure affects enzyme activity, parameters of enzyme immobilization and POT synthesis conditions were optimized. Optimal monomer concentration for POT film synthesis was determined as 40 mM and the scanning rate was determined as 50 mV/s. As for the immobilization process results, GOD, glutaraldehyde (GAL) and chitosan (Chi) concentrations were decided as 2 mg/ml 0.10%, and 0.5% for the Pt/POT electrode. Zinc ferrite nanoparticle (ZnFe2O4NP) was immobilized together with POT film in the presence of GOD enzyme. It was revealed that ZnFe2O4NP increased the current responses and stability of the Pt/POT electrode.

Keywords

Poly(o-toluidine), glucose oxidase, amperometric biosensor, zinc ferrite nanoparticle, conductive polymer

Supporting Institution

Scientific Research Units of The University of Mustafa Kemal

Project Number

8681.

Thanks

This study was funded by Scientific Research Units of The University of Mustafa Kemal in Turkey, Project no: 8681.

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