Determination of Oxidative Stress, Vitamin A, E, C and β-Carotene Levels in the Tissues of Luciobarbus esocinus (Heckel, 1843)

Abstract

In the study was investigated the levels of lipid peroxidation (malondialdehyde; MDA) as oxidative stress, vitamin E (VE), A (VA), C (VC) and β-carotene (βC) in liver, spleen, muscle and kidney tissues of Luciobarbus esocinus. The fish samples used in this study was catched from Keban Dam Lake (Elazıg-Turkey) at August. Analyzes were made in High Performance Liquid Chromotograph (HPLC). The data were analyzed with an Independent-Sample T Test in SPSS 21. It was determined that the levels of parameters analysed was generally higher in liver and spleen compared to kidney and muscle of female and male. The VE, VC and βC levels in liver and kidney were lower in female than male. The levels of VC in spleen were higher in male according to female. The VA level in muscle was lower in male than female, but the level of βC in this tissues was higher in male according to female. Moreover, the MDA level wasn’t different according to sex.

Keywords

• Luciobarbus esocinus
• Vitamins
• Oxidative stress
• Carotene

Luciobarbus esocinus (Heckel, 1843)’un Dokularındaki Oksidatif Stress, Vitamin A, E, C ve β-Karoten SeviyelerininTespiti

Abstract

Bu çalışmada Luciobarbus esocinus’un karaciğer, dalak, kas ve böbrek dokularında oksidatif stres olarak lipid peroksidasyon (malondialdehit-MDA), vitamin E (VE), A (VA), C (VC) ve β-karoten (βC) seviyeleri araştırıldı. Çalışmada kullanılan balık örnekleri Ağustos ayında Keban Baraj Gölü’nden yakalandı. Analizler yüksek performanslı likit kromotografisinde yapıldı (HPLC). Veriler SPSS 21’deIndependent-Sample T and Duncan Testi’nde analiz edildi. Genel olarak analiz yapılan parametrelerin düzeyleri erkek ve dişilerin böbrek ve kasına göre karaciğer ve dalağında daha yüksekti. Karaciğer ve böbrekteki VE, VC ve βC seviyeleri erkeklere göre dişilerde daha düşüktü. Dalaktaki VC seviyesi dişiye göre erkekte daha yüksekti. Kastaki VA seviyesi dişiye göre erkeklerde daha düşüktü. Fakat bu dokudaki βC’nin seviyesi dişiye göre erkekte daha yüksekti. Bunlara ilave olarak bütün dokulardaki MDA seviyesi cinsiyete göre farklılık göstermedi.

Keywords

• Luciobarbus esocinus
• Vitamin
• Oksidatif Stres
• Karoten

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