Atık Sulardan İzole Edilen Pseudomonas Spp.’ lerin Ekzopolisakkarit Üretimine Bazı Ağır Metallerin Etkisi

Öz

Bu çalışmada atık su arıtma havuzlarından alınan örneklerden 50 adet Pseudomonas spp. izole edilmiştir. İzolatların; koloni morfolojileri, Gram boyama sonuçları ve VITEK 2 Compact 30 (biomerieux) cihazında yapılan tür tanımlamaları sonucunda 27 adet Pseudomonas aeruginosa ve 23 adet P. stutzeri oldukları tespit edilmiştir. Tanımlanan izolatlar 10 ppm Cr(VI), Cd(II), Cu(II) ve Mn(II) konsantrasyonlarına maruz bırakılarak bu metallere karşı canlılıkları tespit edilmiş ve her metal için canlılığını en çok koruyan izolatlar seçilmiştir. Aynı zamanda izolatların canlılık değerlerine göre uygulanacak metallerin 4 farklı derişimi belirlenmiştir. Seçilen izolatlara belirlenen metal konsantrasyonları verilerek yüzde ölüm değerleri tespit edilmiş ve LC50 değerleri hesaplanmıştır. Her metal için seçilen izolatlar, belirlenen konsantrasyonlarda metale maruz bırakılarak EPS izolasyonu gerçekleştrilmiştir. 15 ppm Cr(VI) konsantrasyonunda en yüksek EPS üretiminin P.stutzeri S46 izolatında (74,68 mg/L), en düşük EPS üretiminin ise P.stutzeri S18 izolatında olduğu (39,37 mg/L) tespit edilmiştir. 40 ppm Cd(II) konsantrasyonunda en yüksek EPS üretiminin P.stutzeri S23 izolatında (60,93 mg/L), en düşük EPS üretiminin ise P.aeruginosa S10 izolatında olduğu (40,93 mg/L) belirlenmiştir. 80 ppm Cu(II) konsantrasyonunda en yüksek EPS üretiminin P.stutzeri S45 izolatında (74,06 mg/L), en düşük EPS üretiminin ise P. aeruginosa S8 izolatında olduğu (28,28 mg/L) görülmüştür. 400 ppm Mn(II) konsantrasyonunda en yüksek EPS üretiminin P.stutzeri S44 izolatında (46,40 mg/L), en düşük EPS üretiminin ise P.stutzeri S50 izolatında olduğu (26,56 mg/L) tespit edilmiştir. Sonuç olarak artan metal dozlarına maruz kalan Pseudomonas spp. izolatlarının ürettikleri EPS miktarlarında da artış olduğu tespit edilmiştir. Bu çalışma ile atık sulardan izole edilen Pseudomonas spp. izolatlarının bazı ağır metallerin EPS üretimine etkisi biyoteknolojik açıdan vurgulanmıştır.

Anahtar Kelimeler

• Pseudomonas aeruginosa,Pseudomonas stutzeri,Ağır metal

Circular Environmental Policies in The Industrial Production

Öz

In this study, 50 Pseudomonas spp. have been isolated from the instances of waste water purification pool. 27 Pseudomonas aeruginosa and 23 Pseudomonas stutzeri strains were detected according to colony morphologies, Gram staining and type definitions that have been made in the vitek 2 compact 30 (biomerieux) device. Identified strains were exposed by 10 ppm Cr(VI), Cd(II), Cu(II) and Mn(II) concentrations and detected their viabilities against these metals and by this way determined the most resistant strains for each metal. Also, four different concentrations of these metals were determined according to viability of strains. % death values of strains were determined by treated with metal concentrations and LC₅₀ values of metals were calculated. Strains selected for each metal by exposing them to the specified concentrations, EPS isolation has been carried out. In Cr(VI) concentration it has been found that the Pseudomonas stutzeri of the highest EPS production is S46 strains (74.68 mg/L) while the lowest Pseudomonas stutzeri is S18 (39.37 mg/L). In Cd(II) concentration; the Pseudomonas stutzeri of the highest EPS production is S23 (60.93 mg/L), while the lowest EPS production is Pseudomonas aeruginosa S10 strains (40.93 mg/L). In Cu(II) concentration, the Pseudomonas stutzeri of the highest EPS production is S45 strains (76.06 mg/L) while the lowest EPS production is Pseudomonas aeruginosa S8 strains (28.28 mg/L). In Mn (II) concentration, the Pseudomonas stutzeri of the highest EPS production is S44 strains (46.40 mg/L), while the Pseudomonas stutzeri of the lowest EPS production is S50 strains (26.56 mg/L). As a result, exposed to increasing metal dose of Pseudomonas spp., the amount of EPS produced by the strains has been found to be increased. By this study it has been expressed the effect of some heavy metals of Pseudomonas spp strains, which have been isolated from wastewater, to the EPS production in terms of biotechnology.

Anahtar Kelimeler

• Pseudomonas aeruginosa,Pseudomonas stutzeri,Heavy metal,Exopolysaccharide.

Kaynakça

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