Malacosoma castrensis ile İlişkili Kültüre Edilebilir Bağırsak Bakteri Toplulukları: İzolasyon ve 16S rRNA Dizin Analizi ile Tanımlama

Yıl 2023, Cilt: 12 Sayı: 2, 26 - 33, 31.12.2023

Ali Sevim

https://doi.org/10.17100/nevbiltek.1336873

Öz

Pek çok hayvan ve bitki vücutlarının içerisinde simbiyotik mikroorganizmalara sahiptir ve bu ortaklar arasında yakın etkileşimler meydana gelmektedir. Böcekler ise en büyük hayvan grubu oluşturmaktadırlar ve zorunlu mutualizm’den fakültatif parazitizm’e kadar değişen çeşitli endosimbiyotik ilişkileri içermektedirler. Böceklerdeki endosimbiyotik bakterilerin beslenme, üreme, savunma, iletişim, davranış ve gelişim gibi pek çok farklı rolleri bulunmaktadır. Bu çalışmada böceklerdeki simbiyotik bakterileri tanımlamak için model organizma olarak Malacosoma castrensis seçilmiştir. Bu böceğin larvalarında yer alan bakteriler izole edilmiş ve 16S rRNA sekans analizi ile tanımlanmıştır. Toplam olarak 6 adet bakteri izole edilmiş ve bunlar Staphylococcus sp. MM-1, Micrococcus sp. MM-2, Rhodococcus sp. MM-3, Arthrobacter citreus MM-4, Bacillus sp. MM-5 ve Pseudomonas sp. MM-6 olarak tanımlanmıştır. Elde edilen sonuçların böcek-bakteri ilişkilerinin belirlenmesinde ve böceklerdeki endosimbiyotik bakterilerinin rollerinin aydınlatılmasında faydalı olacağı düşünülmektedir.

Anahtar Kelimeler

Malacosoma castrensis, Bakteri, Simbiyosis, 16S rRNA

Kaynakça

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