Atomoksetitin fare izole vas deferens kontraktilitesi üzerine etkisi

Year 2024, , 129 - 135, 30.10.2024

Seçkin Engin , Mehmet Kağan Altınbaş

https://doi.org/10.33719/nju1538778

Abstract

Amaç: Seçici bir noradrenalin geri alım inhibitörü olan atomoksetin (ATX), dikkat eksikliği hiperaktivite bozukluğunun tedavisinde yeterli etkinliği ve olumlu güvenlik profiliyle tercih edilen bir ilaçtır. ATX alan hastalarda cinsel yan etki olarak ejakülasyon bozuklukları rapor edilmiştir ve bunların altında yatan mekanizmalar büyük ölçüde bilinmemektedir. Bu çalışma, ATX’e bağlı gelişen ejakülasyon bozukluklarının potansiyel bir mekanizması olarak ATX’in fare izole vas deferens (VD) kontraktilitesi üzerindeki etkisini araştırmayı amaçladı.
Gereç ve Yöntemler: Yetişkin erkek Balb/c farelerden elde edilen prostatic VD striplerinde izole organ banyosu çalışmaları yapıldı. ATX'in (10-6, 10-5, 3x10-5 ve 10-4 M); KCl (80 mM), fenilefrin (PhE, 3x10-4 M), adenozin 5'-trifosfat (ATP, 10-2 M) ve elektriksel alan stimülasyonu (EAS; 100 V, 64 Hz) ile indüklenen VD kasılmaları üzerine etkisi konsantrasyona bağlı olarak değerlendirildi.
Bulgular: 10-6 ve 10-5 M ATX, kasılma yanıtlarını değiştirmedi (p>0.05), ancak daha yüksek ATX konsantrasyonları (3x10-5 veya 10-4 M), KCl, PhE, ATP ve EASile indüklenen önemli ölçüde inhibe etti.
Sonuç: Bu çalışma ilk kez ATX'in fare izole VD kasılma yanıtlarını konsantrasyona bağlı olarak azalttığını gösterdi. Sonuçlarımız ATX’e bağlı gelişen ejakülasyon disfonksiyonunun, ATX'in VD üzerindeki inhibitör etkisi ile ilişkili olabileceğini düşündürmektedir.

Keywords

atomoksetin, ejakülasyon, izole organ banyosu, kasılma, vas deferens

Ethical Statement

Karadeniz Technical University Rectorate Animal Experiments Local Ethics Committee. Date: 15.09.2023 Protocol: 2023/32.

Supporting Institution

This study was supported by a grant from TUBITAK Research Project Support Programme for Undergraduate Students (2209-A 2023/1, Project No. 1919B012306489).

Project Number

1919B012306489

Effect of Atomoxetine on Mouse Isolated Vas Deferens Contractility

Abstract

Objective: Atomoxetine (ATX), a selective noradrenaline re-uptake inhibitor, is a preferred drug with sufficient efficacy and favorable safety profile for the treatment of attention-deficit hyperactivity disorder. Ejaculatory dysfunctions have been reported in the patients receiving ATX as sexual side effect, of which underlying mechanisms are largely unknown. The present study aimed to investigate the effect of ATX on mouse isolated vas deferens (VD) contractility as a potential mechanism of ATX-induced ejaculatory dysfunction.
Material and Methods: Isolated organ bath studies were performed on prostatic parts of VD obtained from adult male Balb/c mice. The effect of ATX (10-6, 10-5, 3x10-5 and 10-4 M) on KCl (80 mM), phenylephrine (PhE, 3x10-4 M), adenosine 5’-triphosphate (ATP, 10-2 M) and electrical field stimulation (EFS; 100 V, 64 Hz)-induced contractions of VD strips were evaluated in concentration dependent manner.
Results: ATX at 10-6 and 10-5 did not alter the contractile responses (p>0.05), however, higher concentrations of ATX (3x10-5 or 10-4 M) significantly inhibited the KCl, PhE, ATP and EFS-induced contractions of VD strips (p<0.05).
Conclusion: The present study demonstrated for the first time that ATX decreased the contractile responses of mouse isolated VD concentration-dependently. Our results suggest that ejaculatory dysfunction might be related to the inhibitory effect on ATX on VD.

Keywords

atomoxetine, contraction, ejaculation, isolated organ bath, vas deferens

Ethical Statement

All experimental procedures was approved by Institutional Animal Care and Use Committee (approval number: 2023/32)

Supporting Institution

This study was supported by a grant from TUBITAK Research Project Support Programme for Undergraduate Students (2209-A 2023/1, Project no. 1919B012306489).

Project Number

1919B012306489

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