Kurutulmuş üzüm posasında fenoliklerin ve antioksidan özelliklerin yakın kızılötesi spektroskopisi ile belirlenmesi

Year 2025, Volume: 14 Issue: 4, 1530 - 1541, 15.10.2025

Hüseyin Ayvaz , Muhammed Ali Doğan

Abstract

Bu çalışmada, kurutulmuş üzüm posası tozlarında biyoaktif özelliklerin belirlenmesinde, yakın kızılötesi (NIR) spektroskopisi ile kısmi en küçük kareler regresyonunun (PLSR) potansiyeli değerlendirilmiştir. Kırmızı–pembe ve mor–siyah üzümlerden elde edilen 24 örnek, toplam monomerik antosiyaninler, fenolikler, flavonoidler, proantosiyanidinler ve antioksidan kapasite açısından CUPRAC (cupric reducing antioxidant capacity) ve DPPH (2,2-difenil-1-pikrilhidrazil) yöntemleriyle analiz edilmiştir. Kimyasal analizler, mor–siyah üzüm posasında fenolik ve antioksidan düzeylerinin daha yüksek olduğunu göstermiştir. NIR spektrumlarından elde edilen PLSR modelleri, tüm parametreler için güçlü kalibrasyon ve çapraz doğrulama ile umut verici sonuçlar ortaya koymuştur. Bulgular, NIR spektroskopisinin geleneksel ıslak kimya yöntemlerine basit, tahribatsız ve çevre dostu bir alternatif sunduğunu göstermektedir. Ayrıca bu yaklaşım, üzüm posasında biyoaktif bileşiklerin izlenmesini sağlayarak tarımsal-endüstriyel yan ürünlerin sürdürülebilir biçimde değerlendirilmesine katkı sunabilir.

Keywords

Üzüm posası , yakın kızılötesi spektroskopi , PLSR , fenolikler , antioksidan aktivite

Quantification of phenolics and antioxidant properties in dried grape pomace by near infrared spectroscopy

Abstract

This study evaluated the potential of near-infrared (NIR) spectroscopy, combined with partial least squares regression (PLSR) to quantify bioactive properties in dried grape pomace powders. Twenty-four samples of red-pink and purple-black grapes were examined for total monomeric anthocyanins, phenolics, flavonoids, proanthocyanidins, and antioxidant capacity utilizing CUPRAC (cupric reducing antioxidant capacity) and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays. Chemical analyses verified higher phenolic and antioxidant contents in purple-black pomace than red-pink. PLSR models from NIR spectra produced promising results, showing strong calibration and cross-validation for all analytes. These outcomes indicate that NIR spectroscopy provides a simple, nondestructive, and eco-friendly alternative to conventional wet chemistry methods. Moreover, the approach offers potential for monitoring bioactive compounds in grape pomace, thereby supporting the valorization of agro-industrial residues as sustainable sources of phenolics and antioxidants for food, nutraceutical, and cosmetic applications.

Keywords

Grape pomace , near-infrared spectroscopy , PLSR , phenolics , antioxidant activity

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