Investigation of Protective Effect of Hypericum Perforatum on Ethanol-induced Acute Gastric Mucosal Injury in Rats

Abstract

Objective: This study was conducted to evaluate the antiapoptototic and antiinflammatory effect of Hypericum perforatum in the rats induced gastric ulcer by absolute ethanol. Materials and Methods: Forty male Wistar albino rats were used in this study. Rats were divided four group, randomly. Group I (Control, n=10), Group II (Ethanol, n=10, 1 mL ethanol, orally, 90 min)  Group III (H. perforatum + ethanol, 1 mL H. perforatum, 120 min; 1 mL ethanol, 90 min, orally), Group IV (Olive oil + ethanol, 1 mL olive oil, 120 min; 1 mL ethanol, 90 min, orally). Gastric ulcer score  was determined in gastric mucosa by morphometrically. Histopatological staining, Immunohistochemical iNOS and PCNA staining, apoptotic TUNEL staining, gastric IL-10 and IL-6 expression (ELISA) and SOD, MDA, CAT levels were assessed in gastric tissue. Results: The results showed that ethanol incuded gastric damage, higher gastric ulcer index score, increased proinflammatory IL-6 level, elevated number of iNOS and TUNEL-positive stained cell number as well as higher MDA level in the group II (ethanol). Pre-treatment of H. perforatum and olive oil (group III and IV) significantly attenuated the gastric lesions as compared to the group II (ethanol). Conclusion: It was concluded that H. perforatum and olive oil may represents a potential therapeutic option to reduce the risk of gastric mucosal ulceration.

Keywords

• Gastric ulcer,Hypericum perforatum,iNOS,TUNEL,Interleukin-6,Interleukin-10

Etanol Uyarımlı Sıçan Akut Mide Mukoza Hasar Modelinde Hypericum Perforatum'un Koruyucu Etkilerinin İncelenmesi

Öz

Amaç: Bu çalışma sıçanlarda absolü alkol ile oluşturulan gastrik ülserde H. perforatum'un antiapoptotik ve antiinflamatuvar etkilerini değerlendirmek amacıyla yapılmıştır. Gereç ve Yöntem: Bu çalışmada 40 adet erkek Wistar albino sıçan kullanılmıştır. Sıçanlar rastgele 4 gruba ayrılmıştır. Grup I (Kontrol grubu, n=10), Grup II (Etanol grubu, n=10, 1 mL etanol, oral yolla, 90 dk), Grup III (H. perforatum+Etanol grubu, n=10, 1 mL H.perforatum, 120 dk; 1 mL etanol, 120 dk, oral yolla), Grup IV (Zeytinyağı+Etanol, 1 mL zeytinyağı, 120 dk; 1 mL etanol, 120 dk, oral yolla). Gastrik ülser skoru mide mukozasında ülserasyon alanlarının ölçülmesiyle hesaplandı. Gastrik dokuda histopatolojik boyamalar, immunohistokimyasal İNOS ve PCNA boyamaları, TUNEL boyaması, IL-6 ve IL-10 ifadeleri (ELISA) ve MDA, SOD ve CAT enzim seviyeleri değerlendirildi. Bulgular:Sonuçlara incelendiğinde, etanol uyarımlı modelde gastrik hasar; yüksek ülser indeksi skoru, yükselmiş proinflamatuvar IL-6 seviyesi, fazla sayıda TUNEL-pozitif hücre, artmış iNOS ifadesi ve yüksek MDA seviyesi bulguları tespit edildi. H. perforatum ve Zeytinyağı ile ön tedavi yapılmış gruplarda gastrik lezyonların önemli derecede azaldığı tespit edildi. Sonuç: Sonuç olarak H. perforatum ve zeytinyağı gastrik mukozal ülserasyon riskini azaltmak için potansiyel bir tedavi seçeneği olabilir.

Anahtar Kelimeler

• Gastrik ülser,Hypericum perforatum,iNOS,PCNA,TUNEL,İnterlökin-6,İnterlökin-10

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