Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar

Year 2020, , 84 - 92, 20.04.2020

Elif Polat

https://doi.org/10.37696/nkmj.679069

Abstract

İki boyutlu (2B) hücre kültürü, hücre temelli araştırmalar için değerli bir yöntemdir ancak in vivo yanıtlar hakkında öngörülemeyen, yanıltıcı veriler sağlayabilir. Son yirmi yılda, hücresel mikro çevrenin (örneğin hücre dışı matris ve interstisyel sıvı) önemine farkındalık artmıştır. 3B hücre kültürü olarak adlandırılan bu yeni hücre kültürü paradigması hızla popülerlik kazanıyor. 2B'den 3B kültür tekniklerine geçiş, fizyolojik olarak daha uygun doku modellerine doğru önemli bir adımdır. 3B hücre kültürleri farklı amaçlar için faklı teknikler sunar ve gereksinime göre kullanıcıların en uygun modeli seçmeleri gerekir. 3B hücre kültürü sistemleri, kök hücre çalışmaları, ilaç keşifleri, kanser araştırmaları, gen ve protein ifade çalışmaları ve daha birçok karmaşık fizyolojik mekanizmanın aydınlatılabilmesi için bu alanlarda kullanıldığı görülmektedir. İn vitro çalışmalar; 3B hücre kültürlerinin ortaya çıkmasıyla, 2B kültürler ile mümkün olmayan karmaşık etkileşimleri incelemek için üstün yapılar sunarlar.

Keywords

Üç boyutlu hücre kültürü, sferoid, skafold, teknikler

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