Erratum
Malathion ve Onosma nigricaule (Boraginaceae)’nin Fare Böbrek Dokusunda Leptin Üzerine Etkilerinin İmmunohistokimyasal Olarak İncelenmesi
Abstract
Özet
Amaç
Bu çalışmada, tarımda bir insektisit olarak kullanılan malathion’un
neden olduğu oksidasyon parametreleri üzerine Onosma nigricaule
(Boraginaceae)’den elde edilen bitkisel özütün böbrek dokusunda
leptin üzerine etkisinin immunohistokimyasal olarak araştırılması
amaçlandı.
Materyal-Metot
Çalışmamızda, toplam 48 adet erkek fare kullanıldı. Gruplar;
kontrol (grup 1), malathion’un taşıt maddesi olan mısır yağı (grup
2), bitki özütünün taşıt maddesi olan serum fizyolojik (grup 3),
malathion (grup 4), Onosma nigricaule (grup 5), ve Onosma
nigricaule + malathion grubu (grup 6) olmak üzere toplam 6 grup
olarak belirlendi. Böbreklerden alınan kesitlere leptin
immunoreaktivitesini belirlemek için Streptavidin-Biotin-Peroksidaz
Kompleks tekniği uygulandı. Hazırlanan kesitlere histolojik
incelemeler için hematoksilen-eosin ve Crossman’ın üçlü boyama
yöntemi kullanıldı.
Bulgular
Kontrol grubu ile taşıt kontrol grupları olan serum fizyolojik grubu ve
mısır yağı grupları ile Onosma nigricaule, malathion ve Onosma
nigricaule + malathion gruplarının böbrek dokularında herhangi bir
lezyon tespit edilmedi. Diğer yandan, kontrol, mısır yağı, serum ve
malathion grubundaki farelerin tubulus proksimalis ve tubulus
distalis epitel hücrelerinde güçlü leptin immunoreaktivite
görülmesine karşın Onosma nigricaule ve Onosma nigricaule +
malathion grubu farelerde ise zayıf leptin immunoreaktivitesi tespit
edildi.
Sonuç
Fareler ile yaptığımız çalışmada, yurdumuzda insektisit olarak
yaygın kullanılan malathion’un fare böbreğinde leptin aktivitelerini
etkilediği görülmüştür.
Anahtar Kelimeler: Böbrek, leptin, malathion, Onosma nigricaule
References
- Dökmeci İ. Toksikoloji. 2. rd edn. Nobel Tıp Kitapevi. 2000; 95-99. 2. Gasiewiez TA. Nitro compounds and related phenolic pesticides. In: Hayes WJ Jr.& Laws ER Jr ed. Hand book of pesticide toxicology. Academic Press, San Diego, CA. 1991; 3: 1191-269. 3. Ellenhorn MJ, Schonwald S, Ordog G. Ellenhorn's Medical Toxicolgy; Diagnosis and Treatment of Human poisoning. Baltimore: Williams & Wilkins, 1997;1614-1630. 4. Larsam MM, Annabi AB, Rezg R. Effect of shorttime malatiyon administration on glucose homeostasis in Wistar rat. Pestic. Biochem. Phys. 2008; 92: 114-119. 5. Buratti FM, D’Aniello A, Volpe MT. Malathion bıoaktivation ın the human liver: the contribution of different cytochrome p450 ısoforms. Drug Metabolism And Disposition. 2005; 33: 295-302. 6. Dere E, Bakır S, Atalay A. Malathion’un karaciğer, böbrek ve ince bağırsak alkalen fosfataz aktivitesi üzerine etkisi. Turkish Journal of Zoology. 1999; 23: 709-713. 7. Friedman JM, Halaas JF. Leptin and the regulation of body weight in mammals. Nature. 1998; 395: 763-770. 8. Zhang Y, Proenca R, Maffei M ve ark . Positional cloning of the mouse obese gene and its human homologue. Nature. 1994; 372: 425-431. 9. Kaplan LM . Leptin, obesity and liver disease. Gastroenterology. 1998; 115: 997-1001. 10. Kaçar C, Arı UÇ. Leptinin inek ve koyunlarda enerji metabolizması ve üreme fizyolojisi üzerine etkileri. Kafkas Üniv Vet Fak Derg. 2007; 13 (2): 209-213. 11. Prins JB, O Rahilly S. Regulation of adipose cell number in man. Clin Sci. 1997; 92: 3-11. 12. Wilding J, Widdowson P, Williams G. Neurobiology. Br Med Bull. 1997; 53: 286-306. 13. Murakami T, Yamashita T, Lida M ve ark. A short from of leptin receptor performs signal transduction. Biochem Biophys Res Commun. 1997; 231: 26-29. 14. Sharma K, Considine RV, Michael B ve ark. Plasma leptin is partly cleared by the kidney and is elevated in hemodialysis patients. Kidney Int. 1997, 51: 1980-1985. 15. Özgen U, Miloğlu FD, Bulut G. Quantitative determination of shikonin derivatives with UV-Vis spectrophotometric methods in roots of Onosma nigricaule. Rev. Anal. Chem. 2011; 30: 59-63,. 16. Kumar N, Gutpa KA, Prakash D ve ark . Hypoglycemic activity of Onosma hispidum (Ratanjot). International Journal of Diabetes in Developing Countrie. 2010; 30: 213-216,. 17. Rinner B, Kretschmer N, Knausz H ve ark. A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner. Journal of Ethnoparmacology. 2010; 129: 182-188,. 18. Erdağ D. Malatiyon verilen farelerde oksidasyon parametreleri üzerine allium czelghauricum (liliaceae), lathyrus karsianus (fabaceae) ve onosma nigricaule (boraginaceae)’den elde edilen ekstraktların etkileri. Kafkas Üniversitesi, Sağlık Bilimleri Enstitüsi, Doktora Tezi, 2012. 19. Tru LD: Principles of immunohistochemistry. Editör: True L.D. Atlas of Diagnostic immunohistopathology. Newyork Press, 1990; 1- 31, USA. 20. Crossmonn G. A modification of Mallory's connective tissue stain with a discussion of the principles involved. Anat Rec, 1937; 69: 33-38. 21. Zık B. Uriner Sistem. In, Özer A (Ed): Veteriner Özel Histoloji. II. Baskı. Nobel Yayın, 2010; 197- 218. 22. Kalender S, Kalender Y, Ogutcu A ve ark. Endosulfan-induced cardiotoxicity and free radical metabolism in rats: The protective effect of vitamin E. Toxicology. 2004; 3: 227-235. 23. Sulak O, Altuntaş I, Karahan N ve ark. Nephrotoxicity in rats induced by organophosphate insecticide methidathion and ameliorating effects of vitamins E and C. Pestic. Biochem. Phys. 2005; 83: 21-28. 24. Freeman BA, Crapo JD. Biology of disease: free radicals and tissue injury. Lab. Invest. 1982; 47: 412-426. 25. Glusczak L, Miron DDS, Moraes BS ve ark. Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia Quelen). Comparative Biochemistry and Physiology, Part C. 2007; 146: 519–524. 26. Keramati V, Jamili S, Ramin M. Effect of Diazinon on Catalase Antioxidant Enzyme Activity in Liver Tissue of Rutilus rutilus. Journal of Fisheries and Aquatic Science. 2010; 5:368-367. 27. Serradeil-Le Gal C, Raufaste D, Brossard G ve ark. Characterization and localization of leptin receptors in the rat kidney. FEBS Lett. 1997; 404:185–191. 28. Hama H, Saito A, Takeda T ve ark. Evidence ındicating that renal tubular metabolism of leptin ıs mediated by megalin but not by the leptin receptors. Endocrinology. 2004; 145:3935–3940. 29. Rosenbaum M, Libel RL, Hırsch J. Obesity. N Engl J Med. 1997; 337:396-407. 30. Ruige JB, Dekker JM, Blum WF. Leptin and variables of body adiposity, energy balance and insülin resistance in a population based study. Diabetes Care. 1999; 22:1097-1104,. 31. Farooqi S, Rau H, Whitehead J. Ob gene mutations and obesity. Proc Nutr Soc. 1998, 57: 471–475. 32. Montague CT, Farooqi IS, Whitehead JP. Congenital leptin deficiency is associated with severe early-onset obesity in humans. Nature. 1997, 387: 903–908. 33. O’Rahilly S. Life without leptin. Nature. 1998, 392: 330–331. 34. Polito A, Fabbri A, Luzzi A: Bazal metabolic rate in anorexia nervoza : relation to Body compozsition and leptin concentrations. Am J Clin Nutr. 2000, 71:1495-1502.
Year 2019,
Volume: 7 Issue: 1, 46 - 46, 22.04.2019
Abstract
References
- Dökmeci İ. Toksikoloji. 2. rd edn. Nobel Tıp Kitapevi. 2000; 95-99. 2. Gasiewiez TA. Nitro compounds and related phenolic pesticides. In: Hayes WJ Jr.& Laws ER Jr ed. Hand book of pesticide toxicology. Academic Press, San Diego, CA. 1991; 3: 1191-269. 3. Ellenhorn MJ, Schonwald S, Ordog G. Ellenhorn's Medical Toxicolgy; Diagnosis and Treatment of Human poisoning. Baltimore: Williams & Wilkins, 1997;1614-1630. 4. Larsam MM, Annabi AB, Rezg R. Effect of shorttime malatiyon administration on glucose homeostasis in Wistar rat. Pestic. Biochem. Phys. 2008; 92: 114-119. 5. Buratti FM, D’Aniello A, Volpe MT. Malathion bıoaktivation ın the human liver: the contribution of different cytochrome p450 ısoforms. Drug Metabolism And Disposition. 2005; 33: 295-302. 6. Dere E, Bakır S, Atalay A. Malathion’un karaciğer, böbrek ve ince bağırsak alkalen fosfataz aktivitesi üzerine etkisi. Turkish Journal of Zoology. 1999; 23: 709-713. 7. Friedman JM, Halaas JF. Leptin and the regulation of body weight in mammals. Nature. 1998; 395: 763-770. 8. Zhang Y, Proenca R, Maffei M ve ark . Positional cloning of the mouse obese gene and its human homologue. Nature. 1994; 372: 425-431. 9. Kaplan LM . Leptin, obesity and liver disease. Gastroenterology. 1998; 115: 997-1001. 10. Kaçar C, Arı UÇ. Leptinin inek ve koyunlarda enerji metabolizması ve üreme fizyolojisi üzerine etkileri. Kafkas Üniv Vet Fak Derg. 2007; 13 (2): 209-213. 11. Prins JB, O Rahilly S. Regulation of adipose cell number in man. Clin Sci. 1997; 92: 3-11. 12. Wilding J, Widdowson P, Williams G. Neurobiology. Br Med Bull. 1997; 53: 286-306. 13. Murakami T, Yamashita T, Lida M ve ark. A short from of leptin receptor performs signal transduction. Biochem Biophys Res Commun. 1997; 231: 26-29. 14. Sharma K, Considine RV, Michael B ve ark. Plasma leptin is partly cleared by the kidney and is elevated in hemodialysis patients. Kidney Int. 1997, 51: 1980-1985. 15. Özgen U, Miloğlu FD, Bulut G. Quantitative determination of shikonin derivatives with UV-Vis spectrophotometric methods in roots of Onosma nigricaule. Rev. Anal. Chem. 2011; 30: 59-63,. 16. Kumar N, Gutpa KA, Prakash D ve ark . Hypoglycemic activity of Onosma hispidum (Ratanjot). International Journal of Diabetes in Developing Countrie. 2010; 30: 213-216,. 17. Rinner B, Kretschmer N, Knausz H ve ark. A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner. Journal of Ethnoparmacology. 2010; 129: 182-188,. 18. Erdağ D. Malatiyon verilen farelerde oksidasyon parametreleri üzerine allium czelghauricum (liliaceae), lathyrus karsianus (fabaceae) ve onosma nigricaule (boraginaceae)’den elde edilen ekstraktların etkileri. Kafkas Üniversitesi, Sağlık Bilimleri Enstitüsi, Doktora Tezi, 2012. 19. Tru LD: Principles of immunohistochemistry. Editör: True L.D. Atlas of Diagnostic immunohistopathology. Newyork Press, 1990; 1- 31, USA. 20. Crossmonn G. A modification of Mallory's connective tissue stain with a discussion of the principles involved. Anat Rec, 1937; 69: 33-38. 21. Zık B. Uriner Sistem. In, Özer A (Ed): Veteriner Özel Histoloji. II. Baskı. Nobel Yayın, 2010; 197- 218. 22. Kalender S, Kalender Y, Ogutcu A ve ark. Endosulfan-induced cardiotoxicity and free radical metabolism in rats: The protective effect of vitamin E. Toxicology. 2004; 3: 227-235. 23. Sulak O, Altuntaş I, Karahan N ve ark. Nephrotoxicity in rats induced by organophosphate insecticide methidathion and ameliorating effects of vitamins E and C. Pestic. Biochem. Phys. 2005; 83: 21-28. 24. Freeman BA, Crapo JD. Biology of disease: free radicals and tissue injury. Lab. Invest. 1982; 47: 412-426. 25. Glusczak L, Miron DDS, Moraes BS ve ark. Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia Quelen). Comparative Biochemistry and Physiology, Part C. 2007; 146: 519–524. 26. Keramati V, Jamili S, Ramin M. Effect of Diazinon on Catalase Antioxidant Enzyme Activity in Liver Tissue of Rutilus rutilus. Journal of Fisheries and Aquatic Science. 2010; 5:368-367. 27. Serradeil-Le Gal C, Raufaste D, Brossard G ve ark. Characterization and localization of leptin receptors in the rat kidney. FEBS Lett. 1997; 404:185–191. 28. Hama H, Saito A, Takeda T ve ark. Evidence ındicating that renal tubular metabolism of leptin ıs mediated by megalin but not by the leptin receptors. Endocrinology. 2004; 145:3935–3940. 29. Rosenbaum M, Libel RL, Hırsch J. Obesity. N Engl J Med. 1997; 337:396-407. 30. Ruige JB, Dekker JM, Blum WF. Leptin and variables of body adiposity, energy balance and insülin resistance in a population based study. Diabetes Care. 1999; 22:1097-1104,. 31. Farooqi S, Rau H, Whitehead J. Ob gene mutations and obesity. Proc Nutr Soc. 1998, 57: 471–475. 32. Montague CT, Farooqi IS, Whitehead JP. Congenital leptin deficiency is associated with severe early-onset obesity in humans. Nature. 1997, 387: 903–908. 33. O’Rahilly S. Life without leptin. Nature. 1998, 392: 330–331. 34. Polito A, Fabbri A, Luzzi A: Bazal metabolic rate in anorexia nervoza : relation to Body compozsition and leptin concentrations. Am J Clin Nutr. 2000, 71:1495-1502.
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Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Correction |
| Authors | |
| Publication Date | April 22, 2019 |
| Published in Issue | Year 2019 Volume: 7 Issue: 1 |