Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil

Abstract

The present study describes the antioxidant and antimicrobial activities of the essential oil of Pulicaria incisa (Lam.), obtained from the aerial part harvested from Oulad Embarek (Beni Mellal-Khenifra region located in the center in Morocco). The P. incisa essential oil presents IC50 using DPPH of 105.99 ± 4.25 μg/mL and an EC50 using FRAP of 87.21 ± 3.1 μg/mL. The antimicrobial activity of the oil was evaluated for five microorganisms. The results obtained revealed different degrees of sensitivity of the bacterial strains tested. The Allorhizobium vitis (S4) and Agrobacterium tumefaciens( C58) strains are the most sensitive to the investigated oil, with inhibition diameters greater than 20 mm and a MIC and CMB of 0.25 (v/v) and 0.5 (v/v) respectively.

Keywords

• Pulicaria incise (Lam.)
• Antioxidant activity
• Antibacterial
• Essential oil
• Sensitivity

References

1. Abd El-Gleel, W., & Hassanien, M. 2012. Antioxidant properties and lipid profile of Diplotaxis harra, Pulicaria incisa and Avicennia marina. Acta alimentaria, 41(2), 143-151. Ait-Ouazzou, A., S. Lorán, M. Bakkali, A. Laglaoui, C. Rota, A. Herrera, R. Pagán and P. Conchello. 2011. Chemical composition and antimicrobial activity of essential oils of Thymus algeriensis, Eucalyptus globulus and Rosmarinus officinalis from Morocco. Journal of the Science of Food and Agriculture, 91: 2643-2651. Al-Hajj, N. Q. M., Wang, H., Gasmalla, M. A., Ma, C., Thabit, R., Rahman, M. R. T., & Tang, Y. 2014. Chemical composition and antioxidant activity of the essential oil of Pulicaria inuloides. Journal of Food and Nutrition Research, 2(5), 221-227. Amer, M.M.A., Ramadan, M.F. & Abd El-Gleel, W. 2007: Impact of Pulicaria incisa, Diplotaxis harra and Avicennia marina as hypocholesterolemic agent. Dt. Lebensmittel Rdsch., 103, 320–327. Bouaichi, A., Benkirane, R., Habbadi, K., Benbouazza, A., & Achbani, E. H. 2015. Antibacterial activities of the essential oils from medicinal plants against the growth of Pseudomonas savastanoi pv. savastanoi causal agent of olive knot. J Agric Vet Sci, 8(12), 415. Burits, M., Bucar, F., 2000. Antioxidant activity of Nigella sativa essential oil. Phytother. Res.14, 323–328. Chaib, F., Allali, H., Bennaceur, M., & Flamini, G. (2017). Chemical composition and antimicrobial activity of essential oils from the aerial parts of Asteriscus graveolens (FORSSK.) LESS. and Pulicaria incisa (LAM.) DC.: Two asteraceae herbs growing wild in the hoggar. Chemistry & biodiversity, 14(8), e1700092. Cuendet, M., Hostettmann, K., and Potterat, O. 1997. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helv. Chim. Acta 80, 1144-1152. Ghazanfar SA. Handbook of Arabian Medicinal Plants; CRC Press: Boca Raton, FL, USA, 1994. Harififar, F., Moshafi. M.H., Mansouri. S.H., Khodashenas, M., Khoshnoodi, M., 2007. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control.18, 800–805. Ibn Sina. EL-Kanoun fil-Tib. Halaby Co., Cairo, Egypte. Vol. 3, 1971. Ismaili, H., L. Milella, S. Fkih-Tetouani, A. Ilidrissi, A. Camporese, S. Sosa, G. Altinier, R. Della Loggia and R. Aquino. 2004. In vivo topical anti-inflammatory and in vitro antioxidant activities of two extracts of Thymus satureioides leaves. Journal of Ethnopharmacology, 91: 31-36. Elegami, A.A.B., Ishag, K.E., Mahmoud, E.N., Alfutuh, I.M.A. & Karim, E.I.A. 1994: Insecticidal activity of Pulicaria undulata oil. Fitoterapia, 65, 82–83. El-Kamali, H.H., Ahmed, A.H., Mohammed, A.S., Yahia, A.A.M., El-Tayeb, I. & Ali, A.A. 1998: Antibacterial properties of essential oils from Nigella sativa seeds, Cymbopogon citratus leaves and Pulicaria undulata aerial parts. Fitoterapia, 69, 77–78. Elmann, A., Telerman, A., Mordechay, S., Erlank, H., & Ofir, R. 2012. Antioxidant and astroprotective effects of a Pulicaria incisa infusion. Oxidative medicine and cellular longevity. Ezoubeiri, A., Gadhi, C. A., Fdil, N., Benharref, A., Jana, M., & Vanhaelen, M. 2005. Isolation and antimicrobial activity of two phenolic compounds from Pulicaria odora L. Journal of ethnopharmacology, 99(2), 287-292. Kersters, K., De Ley, J., Sneath, P. H. A., & Sackin, M. 1973. Numerical taxonomic analysis of Agrobacterium. Microbiology, 78(2), 227-239. Kirby A. J., Schmidt R. J., 1997. The antioxidant activity of Chinese herbs for eczema and of placebo herbs ÐI. J. Ethnopharmacol. 56, 103-108. Mansour RMA, Ahmed AA, Melek FR, Saleh NAM. 1990: The flavonoids of Pulicaria incisa. Fitoterapia 61:186–187. Mossa, J.S., Hifnaw y, M.S., Al-Yahya, M.A., Al-Meshal, I.A. & Mekkaw i, A.G. 1987: Aromatic plants of Saudi Arabia part 8, GC/Ms analysis of essential oils of Pulicaria arabica and Pulicaria undulata. Int. J. Crude Drug Res., 25, 113–119. Nabiel, A.M.S. 2003: Global phytochemistry: the Egyptian experience. Phytochemistry, 63, 239–241. Oyaizu, M., 1986. Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 44, 307–315. Popoff, M. Y., K. Kersters, M. Kiredjian, I. Miras and C. Coynault. 1984. Taxonomic position of Agrobacterium strains of hospital origin. Annals of the Pasteur Institute / Microbiology, 135: 427-442. Ramadan, M. F., Amer, M. M. A., Mansour, H. T., Wahdan, K. M., El-Sayed, R. M., El-Sanhoty, S., & El-Gleel, W. A. 2009. Bioactive lipids and antioxidant properties of wild Egyptian Pulicaria incise, Diplotaxis harra, and Avicennia marina. Journal für Verbraucherschutz und Lebensmittelsicherheit, 4(3-4), 239. Remmal, A., T. Bouchikhi, K. Rhayour, M. Ettayebi and A. Tantaoui-Elaraki. 1993. Improved method for the determination of antimicrobial activity of essential oils in agar medium. Journal of Essential Oil Research, 5: 179-184. Rustaiyan, A., Habibi, Z., Saberi, M. & Jakupovic, J. 1991: A nor-guaianolide and a glaucolide-like eudesmanolide from Pulicaria undulata. Phytochemistry, 30, 2405–2406. Sahin, F., Güllüce, M., Daferera, D., Sökmen, A., Sökmen, M., Polissiou, M., 2004. Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia region of Turkey. Food Control 15,549–557. Shabana, M. M., Mirhom, Y. W., Genenah, A. A., Aboutabl, E. A., & Amer, H. A. 1990: Study into wild Egyptian plants of potential medicinal activity. Ninth communication: hypoglycaemic activity of some selected plants in normal fasting and alloxanised rats. Archiv fur experimentelle Veterinarmedizin, 44(3), 389-394. Saleh N. A. M., 2003. “Global phytochemistry: the Egyptian experience,” Phytochemistry, vol. 63, no. 3, pp. 239–241. Song, A., Y. Wang and Y. Liu. 2009. Study on the chemical constituents of the essential oil of the leaves of Eucalyptus globulus Labill from China. Asian Journal of Traditional Medicines, 4: 34-140. Williams CA, Harborne JB, Greenham JR, Grayer RJ, Kite GG, Eagles J. 2003: Variation in lipophilic and vacuolar flavonoids among European Pulicaria species. Phytochemistry. 64, 275-283.