SPME/GC-MS Analysis of Volatile Organic Compounds from Origanum acutidens (Hand.-Mazz.) Ietsw. - An Endemic Species in Turkey

Year 2021, , 18 - 26, 30.06.2021

Yavuz Bülent Köse , Nagehan Saltan , Mine Kürkçüoğlu

https://doi.org/10.37929/nveo.909788

Abstract

In this present study, it was aimed to determine the composition of the essential oil obtained by water distillation from the aerial parts of Origanum acutidens. The oil was analysed by GC-FID and GC/MS. Carvacrol (69.3%), p-cymene (8.3%), y-terpinene (6.3%), β-caryophyllene (2.7%) and borneol (2.2%) were found as main constituents. In addition, headspace-solid phase microextraction using a Polydimethylsiloxane-Divinylbenzene (PDMS/DVB – 65 μm) fiber of O. acutidens was performed and analysed by GC-FID and GC/MS. The main components were identified as: carvacrol (44.0%), p-cymene (25%), ϒ-terpinene (8%), β-caryophyllene (6.1%) and borneol (6.1%), respectively.

Keywords

Origanum acutidens, essential oil, carvacrol, y-terpinene, β-caryophyllene, Lamiaceae, HS-SPME

References

• Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry (Vol. 456): Allured publishing corporation Carol Stream, IL.
• Baser, K.H.C. & Kırımer, N. (2017). Essential Oils of Anatolian Lamiaceae – An Update, Lamiaceae 2017 Abstracts, Natural Volatiles Essential Oils, 4, 2-8.
• Baytop, T. (1999). Therapy with medicinal plants in Turkey; today and in future. In: Istanbul University Press, Istanbul. Bendahou, M., Muselli, A., Grignon-Dubois, M., Benyoucef, M., Desjobert, J.-M., Bernardini, A.-F., & Costa, J. (2008).
• Antimicrobial activity and chemical composition of Origanum glandulosum Desf. essential oil and extract obtained by microwave extraction: Comparison with hydrodistillation. Food Chemistry, 106(1), 132-139.
• Cosge, B., Turker, A., Ipek, A., & Gurbuz, B. (2009). Chemical compositions and antibacterial activities of the essential oils from aerial parts and corollas of Origanum acutidens (Hand.-Mazz.) Ietswaart, an endemic species to Turkey. Molecules, 14(5), 1702-1712.
• Daferera, D. J., Ziogas, B. N., & Polissiou, M. G. (2003). The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis. Crop protection, 22(1), 39-44.
• Daouk, R. K., Dagher, S. M., & Sattout, E. J. (1995). Antifungal activity of the essential oil of Origanum syriacum L. Journal of Food Protection, 58(10), 1147-1149.
• Davis, P. (1982). Flora of Turkey and East Aegean Islands (Vol. 7). Edinburg Edinburg University Press
• Esen, G., Azaz, A. D., Kurkcuoglu, M., Baser, K. H. C., & Tinmaz, A. (2007). Essential oil and antimicrobial activity of wild and cultivated Origanum vulgare L. subsp. hirtum (Link) letswaart from the Marmara region, Turkey. Flavour and Fragrance Journal, 22(5), 371-376.
• Goze, I., Alim, A., Cetinus, S. A., Cetin, A., Durmus, N., Atas, A. T., & Vural, N. (2010). In vitro antimicrobial, antioxidant, and antispasmodic activities and the composition of the essential oil of Origanum acutidens (Hand.-Mazz.) Ietswaart. Journal of medicinal food, 13(3), 705-709.
• Gulec, A. K., Erecevit, P., Yuce, E., Arslan, A., Bagci, E., & Kirbag, S. (2014). Antimicrobial Activity of the Methanol Extracts and Essential Oil with the Composition of Endemic Origanum acutidens (Lamiaceae). Journal of Essential Oil Bearing Plants, 17(2), 353-358.
• Hochmuth, D. H. (2008). MassFinder-4: Hochmuth Scientific Consulting: Hamburg, Germany
• Ietswaart, J. H., & Ietswaart, J. (1980). A taxonomic revision of the genus Origanum (Labiatae) (Vol. 4): Citeseer. Karagoz H, K. F. P. (2019). Areas of Utilization of Orıganum acutidens (Hand.-Mazz.) Ietswaart and Carvacrol. International Journal of Current Research and Academic Review, 7(2), 46-55.
• Kordali, S., Cakir, A., Ozer, H., Cakmakci, R., Kesdek, M., & Mete, E. (2008). Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresource Technology, 99(18), 8788-8795.
• McLafferty, F. W., & Stauffer, D. B. (1989). The Wiley/NBS registry of mass spectral data (Vol. 1): Wiley New York.
• Sökmen, M., Serkedjieva, J., Daferera, D., Gulluce, M., Polissiou, M., Tepe, B., . . . Sokmen, A. (2004). In vitro antioxidant, antimicrobial, and antiviral activities of the essential oil and various extracts from herbal parts and callus cultures of Origanum acutidens. Journal of agricultural and food chemistry, 52(11), 3309-3312.
• Tozlu, E., Cakir, A., Kordali, S., Tozlu, G., Ozer, H., & Akcin, T. A. (2011). Chemical compositions and insecticidal effects of essential oils isolated from Achillea gypsicola, Satureja hortensis, Origanum acutidens and Hypericum scabrum against broadbean weevil (Bruchus dentipes). Scientia horticulturae, 130(1), 9-17.
• Vas, G. & Vekey, K. (2004). Solid-phase microextraction: a powerful samplepreparation tool prior to mass spectrometric analysis. Journal Of Mass Spectrometry, 39 ,233-254.