TLC-GC/MS Method for identifying and selecting valuable essential Oil Chemotypes from Wild Populations of Mentha longifolia L.

Year 2017, Volume: 4 Issue: 4, 49 - 61, 29.12.2017

Juan Antonio Llorens-molina , Vicente Castell Sandra Vacas Mercedes Verdeguer

Abstract

Wild populations of aromatic plants can be
considered resources of natural biodiversity for selecting valuable genotypes
in order to obtain well-characterized cultivars with defined essential oil (EO)
profiles suitable for different purposes due to their biological activity.
Often, their high intrapopulational variability requires preliminary screenings
based on a significant number of individuals and, therefore, simple and fast
methods to identify EO profiles are required. For this purpose, a TLC-GC/MS method
has been proposed in order to identify chemotypes occurring in a selected wild
population. Stolons of 50 individuals of Mentha longifolia L were planted and
bred in greenhouse conditions before growing in experimental plots. Individual
sampling and extraction of leaves were performed to determinate TLC individual
profiles. They were validated by preparative TLC and GC/MS analysis of
discriminant spots. Fresh material belonging to each defined TLC profile was
collected in full flowering stage and subjected to SDE extraction and GC/MS
analysis. Five chemotypes were characterized: A (piperitone and piperitenone
oxides); B (piperitone oxide + pulegone); C (α- terpineol acetate + carvone
acetate); D ((E) – dihydrocarvone)
and E (pulegone + isomenthone + menthol). This TLC method was applied to
individuals coming from another population. All of them could be clearly
identified as belonging to chemotype E, which was afterwards confirmed by GC/MS
analysis.

Keywords

TLC, Mentha longifolia, essential oil

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