Antioxidant Activity of Chamomile Essential Oil and Main Components

Year 2018, Volume: 5 Issue: 1, 11 - 16, 23.03.2018

Zeynep Fırat , Fatih Demirci , Betül Demirci

Abstract

The objective of this study was to characterize the antioxidant capacity
of the chamomile (Matricaria recutita L.) essential oil (EO) as well as its major constituents. For this
purpose, Pharmacopoeia grade Matricariae aetheroleum was used after the
analytical evaluation, which was confirmed by GC/MS and GC/FID, respectively.
The main components were identified as α-bisabolol oxide A and B, (E)-b-farnesene, α-bisabolone oxide A,
chamazulene, and α-bisabolol
according the European Pharmacopoeia, respectively. The major constituents α-bisabolol oxide A and
chamazulene were purified using prep-TLC from the EO, and the identification
and quantification of the constituents were confirmed by GC/MS and GC/FID
analyses. Chamomile EO, α-bisabolol
oxide A, (E)- b- farnesene,
chamazulene, and α-bisabolol
were evaluated comparatively for their in vitro antioxidant activities using the
spectrophotometric 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging
microdilution assay. The highest free radical inhibitory activity was observed
for chamazulene followed by α-bisabolol
oxide A, the chamomile EO and (E)-b-farnesene, respectively.

Keywords

Chamomile essential oil, Matricaria recutita L., DPPH antioxidant activity, chamazulene

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