In vitro Antimicrobial Evaulation of Ferulago sandrasica Peșmen & Quézel Herba and Root Essential Oil

Year 2019, Volume: 6 Issue: 4, 13 - 20, 26.12.2019

Melike Sucu Damla Kırcı Betül Demirci Hayri Duman Ceyda Sibel Kılıç İlhan G

Abstract

The study material Ferulago sandrasica Peșmen & Quézel was initially collected from Köyceğiz which is endemic for Turkey. The air dried herbal and root parts were evaluated for their volatile compositions and antimicrobial activity. The essential oils were obtained by hydrodistillation which were subsequently analyzed by GC-FID and GC-MS, respectively. The main components of herba essential oil were identified as α-pinene (26.4%), caryophyllene oxide (6.1%), camphene (5.1%), trans-verbenol (5%); whereas the root essential oil compounds were α-pinene (27.9%), limonene (26.1%), δ-3-carene (14.2%), myrcene (7.6%), terpinolene (11%), respectively. In addition, the essential oils were evaluated for their in vitro antimicrobial activity using a broth microdilution technique against the human pathogens Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Bacillus subtilis, Candida tropicalis, C. parapsilosis. The activity results showed that F. sandrasica could effective against some pathogen organism.

Keywords

Ferulago sandrasica, antimicrobial activity, Apiaceae, root and herba essential oil

Supporting Institution

TUBITAK

Project Number

115S364

Thanks

This study is financially supported by TUBITAK (Project no: 115S364). The authors would like to thank microbiologist Melek Tekgöz (Anadolu University, Deparment of Biology) for her assistance in the experiment.

References

• Akalın E., Demirci B., & Başer K.H.C. (2002). A Chemotaxonomic Study on the Genus Ferulago, Sect. Humiles (Umbelliferae). In: B. Şener (Ed.) Biodiversity (pp. 309-313). Springer, Boston, MA.
• Akalın-Uruşak, E., & Kızılarslan, Ç. (2013). Fruit anatomy of some Ferulago (Apiaceae) species in Turkey. Turkish journal of Botany, 37, 434-445.
• Başer, K. H. B., Demirci, B., Özek, T., Akalın, E., & Özhatay, N. (2002). Micro-Distilled Volatile Compounds from Ferulago Species Growing in Western Turkey. Pharmaceutical Biology, 40(6), 466-471.
• Başer, K. H. C., & Kırımer, N. (2014). Essential Oils of Anatolian Apiaceae - A Profile. Naturel Volatiles & Essential Oils, 1(1), 1-50.
• Bostanlık, S. (2015). Pharmaceutical Botanical Investigations on Ferulago sandrasica Peşmen & Quezel and Ferulago mughlae Peşmen (Apiaceae). M.Sc Thesis. Ankara University Faculty of Pharmacy, Ankara.
• Çelik, A., Arslan, I., Herken, E. N., & Ermiş, A. (2013). Constituents, Oxidant-Antioxidant Profile, and Antimicrobial Capacity of the Essential Oil Obtained from Ferulago Sandrasica Peşmen and Quézel. International Journal of Food Properties, 16, 1655-1662.
• Clinical and Laboratory Standards Institute M7-A7, 2006. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Seventh Edition, CLSI document A. Wayne, Pa. USA. 26(2).
• Davis, P.H. (1997). Flora of Turkey and the East Aegean Islands. Edinburgh: Edinburgh University Press.
• Demirci, F., İşcan, G., Güven, K., Kırımer, N. Demirci, B., & Başer, K. H. C. (2000). Antimicrobial Activities of Ferulago Essential Oils. Zeitschrift für Naturforschung C, 55(11-12), 886-889.
• Dorman, H. D. J., & Deans, S. G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology, 88, 308–316.
• Guo, Q., Liu, K., Deng, W., Zhong, B. Yang, W., & Chun, J. (2018). Chemical composition and antimicrobial activity of Gannan navel orange (Citrus sinensis Osbeck cv. Newhall) peel essential oils. Food Science and Nutrition, 6, 1431-1436.
• Hochmuth, D. H. (2008). MassFinder 4.0, Hochmuth Scientific Consulting, Hamburg, Germany.
• Karakaya, S. (2018). Botanical, Phytochemical, Pharmacological Properties of Ferulago genus. Lap Lambert Academic Publishing.
• Karakaya, S., Delimustafaoğlu-Bostanlık, F., Yılmaz-Oral, D., Gür, S., Kılıç, C. S. (2019). Effect of aerial part and root extracts from Ferulago mughlae Peșmen and Ferulago sandrasica Peșmen & Quézel growing in Turkey on erectile dysfunction in streptozotocin-induced diabetic rats. Journal of Research in Pharmacy, 23 (2): 235-241.
• McLafferty, F. W., & Stauffer, D. B. (1989). The Wiley/NBS Registry of Mass Spectral Data, J Wiley and Sons, New York, USA.
• Mitropoulou, G., Fitsiou, E., Spyridopoulou, K., Tiptiri-Kourpeti, A., Bardouki, H., Vamvakias, M., Panas, P., Chlichlia, K., Pappa, A., & Kourkoutas, Y. (2017). Citrus medica essential oil exhibits significant antimicrobial and antiproliferative activity. Food Science and Technology, 84, 344-352.
• Özkan, A. M. G., Demirci, B., Demirci, F., & Baser, K. H. C. (2008). Composition and Antimicrobial Activity of Essential Oil of Ferulago longistylis Boiss. Fruits. Journal of Essential Oil Research, 20(6), 569-573.
• Rezaie, M., Farhoosh, R., Sharif, A., Asili, J., & Iranshahi, M. (2015). Chemical composition, antioxidant and antibacterial properties of Bene (Pistacia atlantica subsp. mutica) hull essential oil. Journal of Food Science and Technology, 5 2(10):6784–6790.
• Selçuk, S., Özsoy, N. Çelik, B. Ö., & Uruşak, E. A. (2017). Antioxidant and Antimicrobial Activity of Ferulago trojana E. Akalın & Pimenov, Istanbul Journal of Pharmacy. 47(3), 101-106.
• Şenol, S.G., Eroğlu, V., Pelit, N.B., & Bozyel, D. (2018). Ekimia ozcan-secmenii (Apiaceae), a new species from Southwest Anatolia, Turkey. Turkish Journal of Botany, 42, 510-517.