Bioactivity evaluation of the native Amazonian species of Ecuador: Piper lineatum Ruiz & Pav. essential oil

Year 2020, Volume: 7 Issue: 4, 14 - 25, 31.12.2020

Eduardo Valarezo Gabriela Merino Claudia Cruz-erazo Luis Cartuche

https://doi.org/10.37929/nveo.782412

Cited By: 5

Abstract

In the present research, the essential oil from Piper lineatum Ruiz & Pav. was analysed by GC/MS and GC/FID, respectively. A total of thirty-seven chemical compounds were identified, which represented 98.9% of the essential oil composition. The main compounds were apiole (21.5%), safrole (19.2%), and myristicin (13.8%), respectively. The in vitro antimicrobial activity and antifungal activity of the oil was assayed against two Gram positive bacteria, five Gram negative bacteria and two fungi. The essential oil from P. lineatum showed an inhibitory activity against Gram-positive bacterium Klebsiella pneumoniae (ATCC 9997), and against dermatophytic fungus Trichophyton rubrum (ATCC 28188) with a MIC of 500 μg/mL in both cases. The antioxidant activity of essential oil was explored using DPPH and ABTS radical scavenging method, by means of both assays the essential oil showed a weak antioxidant activity. To the best of our knowledge, this is the first report on the chemical composition and biological activity of essential oil from this species.

Keywords

Piper lineatum, Apiole, Safrole, Antimicrobial activity, Antioxidant activity

References

• Adams, R. P. (2007). Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry (4th ed.). Carol Stream, IL: Allured Publishing Corporation.
• Andrade, M. A., das Graças Cardoso, M., de Andrade, J., Silva, L. F., Teixeira, M. L., Valério Resende, J. M., da Silva Figueiredo, A. C., & Barroso, J. G. (2013). Chemical Composition and Antioxidant Activity of Essential Oils from Cinnamodendron dinisii Schwacke and Siparuna guianensis Aublet. Antioxidants, 2(4), 384-397. https://doi.org/10.3390/antiox2040384
• Aprotosoaie, A. C., Luca, V. S., Trifan, A., & Miron, A. (2019). Chapter 7 - Antigenotoxic Potential of Some Dietary Non-phenolic Phytochemicals. In R. Atta ur (Ed.), Studies in Natural Products Chemistry (Vol. 60, pp. 223-297). Elsevier. https://doi.org/https://doi.org/10.1016/B978-0-444-64181-6.00007-3
• Arnao, M. B., Cano, A., & Acosta, M. (2001). The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry, 73(2), 239-244. https://doi.org/10.1016/S0308-8146(00)00324-1
• Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
• Clarke, S. (2008). Essential Chemistry for Aromatherapy (Second ed.). Edinburgh, Scotland: Churchill Livingstone. https://doi.org/10.1016/B978-0-443-10403-9.X0001-5
• Clinical and Laboratory Standards Institute. (2008). Reference method for broth Dilution antifungal susceptibility testing of filamentous fungi; Approved Standard–Second Edition. CLSI document M38-A2. Wayne, PA: Clinical and Laboratory Standards Institute.
• Clinical and Laboratory Standards Institute. (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard–Ninth Edition. CLSI document M07-A9. Wayne, PA: Clinical and Laboratory Standards Institute.
• da Silva, J. K., da Trindade, R., Alves, N. S., Figueiredo, P. L., Maia, J. G. S., & Setzer, W. N. (2017). Essential Oils from Neotropical Piper Species and Their Biological Activities. International journal of molecular sciences, 18(12), 2571. https://doi.org/10.3390/ijms18122571
• Davis, E. W., & Yost, J. A. (1983). The ethnomedicine of the waorani of Amazonian Ecuador. Journal of Ethnopharmacology, 9(2), 273-297. https://doi.org/10.1016/0378-8741(83)90036-3
• Evans Schultes, R. (1985). De plantis toxicariis e mundo novo tropicale commentationes XXXV: Miscellaneous notes on biodynamic plants of the northwest Amazon. Journal of Ethnopharmacology, 14(2), 125-158. https://doi.org/10.1016/0378-8741(85)90083-2
• Hendry, E. R., Worthington, T., Conway, B. R., & Lambert, P. A. (2009). Antimicrobial efficacy of eucalyptus oil and 1,8-cineole alone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilm cultures. Journal of Antimicrobial Chemotherapy, 64(6), 1219-1225. https://doi.org/10.1093/jac/dkp362
• Jaramillo-Colorado, B. E., Pino-Benitez, N., & González-Coloma, A. (2019). Volatile composition and biocidal (antifeedant and phytotoxic) activity of the essential oils of four Piperaceae species from Choco-Colombia. Industrial Crops and Products, 138, 111463. https://doi.org/10.1016/j.indcrop.2019.06.026
• Jørgesen, P. M., & León-Yáñez, S. (1999). Catalogue of the Vascular Plants of Ecuador. Missouri Botanical Garden Press. St. Louis, MO: Missouri Botanical Garden Press.
• León-Yánez, S., Valencia, R., Pitmam, N., Endara, L., Ulloa Ulloa, C., & Navarrete, H. (2019). Libro Rojo de Plantas Endémicas del Ecuador. Publicaciones del Herbario QCA, Pontificia Universidad Católica del Ecuador. Retrieved 04/05 from https://bioweb.bio/floraweb/librorojo
• Mata, A. T., Proença, C., Ferreira, A. R., Serralheiro, M. L. M., Nogueira, J. M. F., & Araújo, M. E. M. (2007). Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices. Food Chemistry, 103(3), 778-786. https://doi.org/10.1016/j.foodchem.2006.09.017
• Missouri Botanical Garden. (2017, 02/24/2020). Angiosperm Phylogeny Website. University of Missouri. Retrieved 29/04/2020 from http://www.mobot.org/MOBOT/research/APweb/
• Molares, S., González, S. B., Ladio, A., & Agueda Castro, M. (2009). Etnobotánica, anatomía y caracterización físico-química del aceite esencial de Baccharis obovata Hook. et Arn. (Asteraceae: Astereae). Acta Botanica Brasilica, 23, 578-589. http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062009000200030&nrm=iso
• Naim, D. M., & Mahboob, S. (2020). Molecular identification of herbal species belonging to genus Piper within family Piperaceae from northern Peninsular Malaysia. Journal of King Saud University - Science, 32(2), 1417-1426. https://doi.org/10.1016/j.jksus.2019.11.036
• NCCLS. (2002). Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved Standard–Second Edition, NCCLS document M27-A2. Wayne, PA: NCCLS.
• NIST 05. (2005). Mass Spectral Library (NIST/EPA/NIH). Gaithersburg, MD National Institute of Standards and Technology.
• NIST. (2020). Libro del Web de Química del NIST, SRD 69. in Base de Datos de Referencia Estándar del NIST Número 69. U.S. Secretary of Commerce. Retrieved 19-05 from http://webbook.nist.gov
• Pineda M., R., Vizcaíno P., S., García P., C. M., Gil G., J. H., & Durango R., D. L. (2012). Chemical composition and antifungal activity of Piper auritum Kunth and Piper holtonii C. DC. against phytopathogenic fungi. Chilean journal of agricultural research, 72(4), 507-515. https://doi.org/10.4067/S0718-58392012000400008
• Ramírez, J., Cartuche, L., Morocho, V., Aguilar, S., & Malagon, O. (2013). Antifungal activity of raw extract and flavanons isolated from Piper ecuadorense from Ecuador [Article]. Brazilian Journal of Pharmacognosy, 23(2), 370-373. https://doi.org/10.1590/S0102-695X2013005000012
• Salehi, B., Zakaria, Z. A., Gyawali, R., Ibrahim, S. A., Rajkovic, J., Shinwari, Z. K., Khan, T., Sharifi-Rad, J., Ozleyen, A., Turkdonmez, E., Valussi, M., Tumer, T. B., Monzote Fidalgo, L., Martorell, M., & Setzer, W. N. (2019). Piper Species: A Comprehensive Review on Their Phytochemistry, Biological Activities and Applications. Molecules (Basel, Switzerland), 24(7), 1364. https://doi.org/10.3390/molecules24071364
• Santana, H. T., Trindade, F. T. T., Stabeli, R. G., Silva, A. A. E., Militão, J. S. T. L., & Facundo, V. A. (2015). Essential oils of leaves of Piper species display larvicidal activity against the dengue vector, Aedes aegypti (Diptera: Culicidae). Revista Brasileira de Plantas Medicinais, 17(1), 105-111. https://doi.org/10.1590/1983-084X/13_052
• Santos, F. A., Silva, R. M., Campos, A. R., de Araújo, R. P., Lima Júnior, R. C. P., & Rao, V. S. N. (2004). 1,8-cineole (eucalyptol), a monoterpene oxide attenuates the colonic damage in rats on acute TNBS-colitis. Food and Chemical Toxicology, 42(4), 579-584. https://doi.org/https://doi.org/10.1016/j.fct.2003.11.001
• Şimşek, M., & Duman, R. (2017). Investigation of Effect of 1,8-cineole on Antimicrobial Activity of Chlorhexidine Gluconate. Pharmacognosy research, 9(3), 234-237. https://doi.org/10.4103/0974-8490.210329
• Stefano, V. D., Pitonzo, R., & Schillaci, D. (2011). Antimicrobial and antiproliferative activity of Athamanta sicula L. (Apiaceae). Pharmacognosy magazine, 7(25), 31-34. https://doi.org/10.4103/0973-1296.75893
• Tene, V., Malagón, O., Finzi, P. V., Vidari, G., Armijos, C., & Zaragoza, T. (2007). An ethnobotanical survey of medicinal plants used in Loja and Zamora-Chinchipe, Ecuador. Journal of Ethnopharmacology, 111(1), 63-81. https://doi.org/10.1016/j.jep.2006.10.032
• Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L., & Hawkins Byrne, D. (2006). Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis, 19(6), 669-675. https://doi.org/10.1016/j.jfca.2006.01.003
• The Plant List. (2013). Piperaceae. Retrieved 04-07 from http://www.theplantlist.org/1.1/browse/A/Piperaceae/
• Tisserand, R., & Young, R. (2014). Essential Oil Safety: A Guide for Health Care Professionals (Second ed.). Churchill Livingstone/Elsevier. https://doi.org/10.1016/C2009-0-52351-3
• Tropicos. (2020). Piper lineatum Ruiz & Pav. Retrieved 07-07 from http://legacy.tropicos.org/Home.aspx
• Valarezo, E., Guamán, M. d. C., Paguay, M., & Meneses, M. A. (2019). Chemical Composition and Biological Activity of the Essential Oil from Gnaphalium elegans Kunth from Loja, Ecuador. Journal of Essential Oil Bearing Plants, 22(5), 1372-1378. https://doi.org/10.1080/0972060X.2019.1682684
• Van Vuuren, S., & Holl, D. (2017). Antimicrobial natural product research: A review from a South African perspective for the years 2009–2016. Journal of Ethnopharmacology, 208, 236-252. https://doi.org/10.1016/j.jep.2017.07.011
• Vargas, L., Velasco-Negueruela, A., Pérez-Alonso, M. J., Palá-Paúl, J., & Vallejo, M. C. G. (2004). Essential Oil Composition of the Leaves and Spikes of Piper carpunya Ruíz et Pavón (Piperaceae) from Peru. Journal of Essential Oil Research, 16(2), 122-123. https://doi.org/10.1080/10412905.2004.9698669
• Yuncker, T. G. (1972). The Piperaceae of Brazil; Piper - Group I, II, III, IV. Brazil: Instituto de Botanica.